Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthri...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/7/1677 |
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| author | Norlaily Ahmad Burcu Colak De-Wen Zhang Martin John Gibbs Michael Watkinson C. Remzi Becer Julien E. Gautrot Steffi Krause |
| author_facet | Norlaily Ahmad Burcu Colak De-Wen Zhang Martin John Gibbs Michael Watkinson C. Remzi Becer Julien E. Gautrot Steffi Krause |
| author_sort | Norlaily Ahmad |
| collection | DOAJ |
| description | Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM. |
| first_indexed | 2024-04-11T12:52:32Z |
| format | Article |
| id | doaj.art-1c006f8c772d41c59ad068198f9f8a65 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:52:32Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-1c006f8c772d41c59ad068198f9f8a652022-12-22T04:23:10ZengMDPI AGSensors1424-82202019-04-01197167710.3390/s19071677s19071677Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of CollagenaseNorlaily Ahmad0Burcu Colak1De-Wen Zhang2Martin John Gibbs3Michael Watkinson4C. Remzi Becer5Julien E. Gautrot6Steffi Krause7School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UKSchool of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UKInstitute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, ChinaSchool of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UKThe Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire ST5 5BG, UKDepartment of Chemistry, University of Warwick, Coventry CV4 7AL, UKSchool of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UKSchool of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UKPeptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.https://www.mdpi.com/1424-8220/19/7/1677protease biosensorcollagenasepoly(ethylene glycol) norborneneQCMhydrogel degradationpeptide cross-linksclick chemistry |
| spellingShingle | Norlaily Ahmad Burcu Colak De-Wen Zhang Martin John Gibbs Michael Watkinson C. Remzi Becer Julien E. Gautrot Steffi Krause Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase Sensors protease biosensor collagenase poly(ethylene glycol) norbornene QCM hydrogel degradation peptide cross-links click chemistry |
| title | Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase |
| title_full | Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase |
| title_fullStr | Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase |
| title_full_unstemmed | Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase |
| title_short | Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase |
| title_sort | peptide cross linked poly ethylene glycol hydrogel films as biosensor coatings for the detection of collagenase |
| topic | protease biosensor collagenase poly(ethylene glycol) norbornene QCM hydrogel degradation peptide cross-links click chemistry |
| url | https://www.mdpi.com/1424-8220/19/7/1677 |
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