Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC50) were detected using the CCK-8 method, and the le...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1117364/full |
| _version_ | 1811170922892099584 |
|---|---|
| author | Duo Feng Duo Feng Duo Feng Shi-qi Zhou Shi-qi Zhou Ya-xi Zhou Ya-xi Zhou Yong-jun Jiang Qiao-di Sun Qiao-di Sun Wei Song Wei Song Qian-qian Cui Qian-qian Cui Wen-jie Yan Wen-jie Yan Jing Wang |
| author_facet | Duo Feng Duo Feng Duo Feng Shi-qi Zhou Shi-qi Zhou Ya-xi Zhou Ya-xi Zhou Yong-jun Jiang Qiao-di Sun Qiao-di Sun Wei Song Wei Song Qian-qian Cui Qian-qian Cui Wen-jie Yan Wen-jie Yan Jing Wang |
| author_sort | Duo Feng |
| collection | DOAJ |
| description | To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC50) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC50 level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed. |
| first_indexed | 2024-04-10T17:05:52Z |
| format | Article |
| id | doaj.art-1c009389a4804c95af471952e823d3e0 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-10T17:05:52Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-1c009389a4804c95af471952e823d3e02023-02-06T04:59:10ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-02-011010.3389/fnut.2023.11173641117364Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cellsDuo Feng0Duo Feng1Duo Feng2Shi-qi Zhou3Shi-qi Zhou4Ya-xi Zhou5Ya-xi Zhou6Yong-jun Jiang7Qiao-di Sun8Qiao-di Sun9Wei Song10Wei Song11Qian-qian Cui12Qian-qian Cui13Wen-jie Yan14Wen-jie Yan15Jing Wang16College of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaInner Mongolia Sankou Biotechnology Co., Ltd., Ordos City, Inner Mongolia, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing, ChinaTo study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC50) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC50 level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed.https://www.frontiersin.org/articles/10.3389/fnut.2023.1117364/fullCistanche deserticolatotal glycosidesHepG2 cellsmitochondrial respirationglycolytic pressure |
| spellingShingle | Duo Feng Duo Feng Duo Feng Shi-qi Zhou Shi-qi Zhou Ya-xi Zhou Ya-xi Zhou Yong-jun Jiang Qiao-di Sun Qiao-di Sun Wei Song Wei Song Qian-qian Cui Qian-qian Cui Wen-jie Yan Wen-jie Yan Jing Wang Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells Frontiers in Nutrition Cistanche deserticola total glycosides HepG2 cells mitochondrial respiration glycolytic pressure |
| title | Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells |
| title_full | Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells |
| title_fullStr | Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells |
| title_full_unstemmed | Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells |
| title_short | Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells |
| title_sort | effect of total glycosides of cistanche deserticola on the energy metabolism of human hepg2 cells |
| topic | Cistanche deserticola total glycosides HepG2 cells mitochondrial respiration glycolytic pressure |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1117364/full |
| work_keys_str_mv | AT duofeng effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT duofeng effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT duofeng effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT shiqizhou effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT shiqizhou effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT yaxizhou effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT yaxizhou effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT yongjunjiang effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT qiaodisun effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT qiaodisun effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT weisong effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT weisong effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT qianqiancui effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT qianqiancui effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT wenjieyan effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT wenjieyan effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells AT jingwang effectoftotalglycosidesofcistanchedeserticolaontheenergymetabolismofhumanhepg2cells |