| Summary: | Bacterial spot (BS) of tomato (<i>S. lycopersicum</i>), caused by <i>Xanthomonas</i> spp., namely <i>X. euvesicatoria</i> (Xeu), is one of the major threats for the production of this crop worldwide. Developing new biocontrol solutions against this disease will allow disease management strategies to be less based on Cu compounds. Nanoclays, such as montmorillonite (NMT), have been under investigation for their antimicrobial activity, or as delivery tools/stabilizers for organic compounds, such as essential oils (EOs), that also possess antimicrobial activity against plant pathogens. This work aims to assess how the application of NMT alone or incorporating <i>S. montana</i> EO on Xeu-infected hosts (var. Oxheart) affects the shoots’ redox status and antioxidant defense mechanisms. In vitro shoots, grown on Murashige and Skoog medium, were divided in two groups, Xeu-infected and uninfected (control) shoots. Shoots of each group were then treated with NMT, <i>S. montana</i> EO, EO-NMT. Results show that the NMT was able to reduce Xeu bacterial amount, while reducing ROS production and keeping the transcript levels of the defense-related genes close to those of the control. When applied to uninfected shoots, the treatments triggered the production of ROS and upregulated the phenylpropanoid and hormone pathway, which suggest that they act as defense elicitors. Globally, the results indicate that NMT has the potential to integrate BS management strategies, due to its antimicrobial activity, and that EO and/or nanoclays could be successfully employed as new disease preventive strategies, since they enhance the healthy shoots’ defense, thus potentially limiting the pathogen establishment.
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