Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i>
Bacterial spot (BS) of tomato (<i>S. lycopersicum</i>), caused by <i>Xanthomonas</i> spp., namely <i>X. euvesicatoria</i> (Xeu), is one of the major threats for the production of this crop worldwide. Developing new biocontrol solutions against this disease will al...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Applied Nano |
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| Online Access: | https://www.mdpi.com/2673-3501/3/3/9 |
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| author | Paulo R. Oliveira-Pinto Nuno Mariz-Ponte Renato L. Gil Edite Cunha Célia G. Amorim Maria C. B. S. M. Montenegro Manuel Fernandes-Ferreira Rose M. O. F. Sousa Conceição Santos |
| author_facet | Paulo R. Oliveira-Pinto Nuno Mariz-Ponte Renato L. Gil Edite Cunha Célia G. Amorim Maria C. B. S. M. Montenegro Manuel Fernandes-Ferreira Rose M. O. F. Sousa Conceição Santos |
| author_sort | Paulo R. Oliveira-Pinto |
| collection | DOAJ |
| description | Bacterial spot (BS) of tomato (<i>S. lycopersicum</i>), caused by <i>Xanthomonas</i> spp., namely <i>X. euvesicatoria</i> (Xeu), is one of the major threats for the production of this crop worldwide. Developing new biocontrol solutions against this disease will allow disease management strategies to be less based on Cu compounds. Nanoclays, such as montmorillonite (NMT), have been under investigation for their antimicrobial activity, or as delivery tools/stabilizers for organic compounds, such as essential oils (EOs), that also possess antimicrobial activity against plant pathogens. This work aims to assess how the application of NMT alone or incorporating <i>S. montana</i> EO on Xeu-infected hosts (var. Oxheart) affects the shoots’ redox status and antioxidant defense mechanisms. In vitro shoots, grown on Murashige and Skoog medium, were divided in two groups, Xeu-infected and uninfected (control) shoots. Shoots of each group were then treated with NMT, <i>S. montana</i> EO, EO-NMT. Results show that the NMT was able to reduce Xeu bacterial amount, while reducing ROS production and keeping the transcript levels of the defense-related genes close to those of the control. When applied to uninfected shoots, the treatments triggered the production of ROS and upregulated the phenylpropanoid and hormone pathway, which suggest that they act as defense elicitors. Globally, the results indicate that NMT has the potential to integrate BS management strategies, due to its antimicrobial activity, and that EO and/or nanoclays could be successfully employed as new disease preventive strategies, since they enhance the healthy shoots’ defense, thus potentially limiting the pathogen establishment. |
| first_indexed | 2024-03-10T00:51:35Z |
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| id | doaj.art-1c030c94144741128910a3336458c5de |
| institution | Directory Open Access Journal |
| issn | 2673-3501 |
| language | English |
| last_indexed | 2024-03-10T00:51:35Z |
| publishDate | 2022-07-01 |
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| series | Applied Nano |
| spelling | doaj.art-1c030c94144741128910a3336458c5de2023-11-23T14:50:13ZengMDPI AGApplied Nano2673-35012022-07-013312614210.3390/applnano3030009Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i>Paulo R. Oliveira-Pinto0Nuno Mariz-Ponte1Renato L. Gil2Edite Cunha3Célia G. Amorim4Maria C. B. S. M. Montenegro5Manuel Fernandes-Ferreira6Rose M. O. F. Sousa7Conceição Santos8Department of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, PortugalDepartment of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalDepartment of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalDepartment of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalDepartment of Chemical Sciences, Faculty of Pharmacy, University of Porto (FFUP), Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, PortugalGreenUPorto & Inov4Agro, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto (FCUP), Rua Campo Alegre, 4169-007 Porto, PortugalBacterial spot (BS) of tomato (<i>S. lycopersicum</i>), caused by <i>Xanthomonas</i> spp., namely <i>X. euvesicatoria</i> (Xeu), is one of the major threats for the production of this crop worldwide. Developing new biocontrol solutions against this disease will allow disease management strategies to be less based on Cu compounds. Nanoclays, such as montmorillonite (NMT), have been under investigation for their antimicrobial activity, or as delivery tools/stabilizers for organic compounds, such as essential oils (EOs), that also possess antimicrobial activity against plant pathogens. This work aims to assess how the application of NMT alone or incorporating <i>S. montana</i> EO on Xeu-infected hosts (var. Oxheart) affects the shoots’ redox status and antioxidant defense mechanisms. In vitro shoots, grown on Murashige and Skoog medium, were divided in two groups, Xeu-infected and uninfected (control) shoots. Shoots of each group were then treated with NMT, <i>S. montana</i> EO, EO-NMT. Results show that the NMT was able to reduce Xeu bacterial amount, while reducing ROS production and keeping the transcript levels of the defense-related genes close to those of the control. When applied to uninfected shoots, the treatments triggered the production of ROS and upregulated the phenylpropanoid and hormone pathway, which suggest that they act as defense elicitors. Globally, the results indicate that NMT has the potential to integrate BS management strategies, due to its antimicrobial activity, and that EO and/or nanoclays could be successfully employed as new disease preventive strategies, since they enhance the healthy shoots’ defense, thus potentially limiting the pathogen establishment.https://www.mdpi.com/2673-3501/3/3/9<i>Satureja montana</i>nanoclay<i>Solanum lycopersicum</i>bacterial spotoxidative stressantimicrobial natural products |
| spellingShingle | Paulo R. Oliveira-Pinto Nuno Mariz-Ponte Renato L. Gil Edite Cunha Célia G. Amorim Maria C. B. S. M. Montenegro Manuel Fernandes-Ferreira Rose M. O. F. Sousa Conceição Santos Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> Applied Nano <i>Satureja montana</i> nanoclay <i>Solanum lycopersicum</i> bacterial spot oxidative stress antimicrobial natural products |
| title | Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> |
| title_full | Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> |
| title_fullStr | Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> |
| title_full_unstemmed | Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> |
| title_short | Montmorillonite Nanoclay and Formulation with <i>Satureja montana</i> Essential Oil as a Tool to Alleviate <i>Xanthomonas euvesicatoria</i> Load on <i>Solanum lycopersicum</i> |
| title_sort | montmorillonite nanoclay and formulation with i satureja montana i essential oil as a tool to alleviate i xanthomonas euvesicatoria i load on i solanum lycopersicum i |
| topic | <i>Satureja montana</i> nanoclay <i>Solanum lycopersicum</i> bacterial spot oxidative stress antimicrobial natural products |
| url | https://www.mdpi.com/2673-3501/3/3/9 |
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