Effect of exercise on chemically-induced colitis in adiponectin deficient mice
<p>Abstract</p> <p>Background</p> <p>Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how e...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-08-01
|
| Series: | Journal of Inflammation |
| Subjects: | |
| Online Access: | http://www.journal-inflammation.com/content/9/1/30 |
| _version_ | 1811248921843859456 |
|---|---|
| author | Saxena Arpit Fletcher Emma Larsen Bianca Baliga Manjeshwar Durstine J Fayad Raja |
| author_facet | Saxena Arpit Fletcher Emma Larsen Bianca Baliga Manjeshwar Durstine J Fayad Raja |
| author_sort | Saxena Arpit |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis.</p> <p>Methods</p> <p>Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water.</p> <p>Results</p> <p>The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED.</p> <p>Conclusions</p> <p>Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis.</p> |
| first_indexed | 2024-04-12T15:36:21Z |
| format | Article |
| id | doaj.art-1c04813552fa4611a0c3641a3eb2e786 |
| institution | Directory Open Access Journal |
| issn | 1476-9255 |
| language | English |
| last_indexed | 2024-04-12T15:36:21Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Inflammation |
| spelling | doaj.art-1c04813552fa4611a0c3641a3eb2e7862022-12-22T03:26:56ZengBMCJournal of Inflammation1476-92552012-08-01913010.1186/1476-9255-9-30Effect of exercise on chemically-induced colitis in adiponectin deficient miceSaxena ArpitFletcher EmmaLarsen BiancaBaliga ManjeshwarDurstine JFayad Raja<p>Abstract</p> <p>Background</p> <p>Inflammatory bowel diseases are associated with increased adiponectin (APN) levels, which may exert pro-inflammatory effects in these individuals. Since habitual exercise may increase APN, the aim of this study was to determine how exercise training affects mice with acute colitis.</p> <p>Methods</p> <p>Male adiponectin knock out (APNKO) and wild type (WT) mice (C57BL/6) were randomly assigned to 4 different groups: 1) Sedentary (SED); 2) Exercise trained (ET); 3) Sedentary with dextran sodium sulfate (DSS) treatment (SED + DSS); and 4) Exercise trained with DSS (ET + DSS). Exercise-trained mice ran at 18 m/min for 60 min, 5d/wk for 4 weeks. Subsequently, the ET + DSS and the SED + DSS mice received 2% DSS in their drinking water for 5 days (d), followed by 5d of regular water.</p> <p>Results</p> <p>The clinical symptoms of acute colitis (diarrhea, stool haemoccult, and weight loss) were unaffected by exercise and there was no difference between the APNKO and WT mice (p > 0.05) except on day 39. However, the clinical symptoms of the DSS-treated APNKO mice were worse than WT mice treated with DSS and had increased susceptibility to intestinal inflammation due to increased local STAT3 activation, higher IL-6, TNF-α, IL-1β and IL-10 levels, and as a result had increased intestinal epithelial cell proliferation (p < 0.05). Exercise training significantly decreased pro-inflammatory cytokines including IL-6, TNF-α and IL-1β (p < 0.05) in the DSS + EX APNKO mice but had no effect on epithelial cell proliferation. Exercise was also found to significantly decrease the phosphorylation expression of STAT3 in both WT and APNKO mice in DSS + EX group when compared to DSS + SED.</p> <p>Conclusions</p> <p>Exercise training may contribute in alleviating the symptoms of acute colitis and APN deficiency may exacerbate the intestinal inflammation in DSS-induced colitis.</p>http://www.journal-inflammation.com/content/9/1/30AdipokinesCytokinesInflammationEpithelial cell proliferationIntestine |
| spellingShingle | Saxena Arpit Fletcher Emma Larsen Bianca Baliga Manjeshwar Durstine J Fayad Raja Effect of exercise on chemically-induced colitis in adiponectin deficient mice Journal of Inflammation Adipokines Cytokines Inflammation Epithelial cell proliferation Intestine |
| title | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
| title_full | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
| title_fullStr | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
| title_full_unstemmed | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
| title_short | Effect of exercise on chemically-induced colitis in adiponectin deficient mice |
| title_sort | effect of exercise on chemically induced colitis in adiponectin deficient mice |
| topic | Adipokines Cytokines Inflammation Epithelial cell proliferation Intestine |
| url | http://www.journal-inflammation.com/content/9/1/30 |
| work_keys_str_mv | AT saxenaarpit effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice AT fletcheremma effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice AT larsenbianca effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice AT baligamanjeshwar effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice AT durstinej effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice AT fayadraja effectofexerciseonchemicallyinducedcolitisinadiponectindeficientmice |