Ablation of microglia does not alter circadian rhythm of locomotor activity
Abstract Microglia, as macrophages in the brain, are responsible for immune responses and synaptic remodeling. Although the function of microglia is regulated by circadian rhythms, it is still unclear whether microglia are involved in the generation and light entrainment of circadian rhythms of beha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-023-01021-1 |
| _version_ | 1797849815221010432 |
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| author | Futaba Matsui Sho T. Yamaguchi Riho Kobayashi Shiho Ito Sakimi Nagashima Zhiwen Zhou Hiroaki Norimoto |
| author_facet | Futaba Matsui Sho T. Yamaguchi Riho Kobayashi Shiho Ito Sakimi Nagashima Zhiwen Zhou Hiroaki Norimoto |
| author_sort | Futaba Matsui |
| collection | DOAJ |
| description | Abstract Microglia, as macrophages in the brain, are responsible for immune responses and synaptic remodeling. Although the function of microglia is regulated by circadian rhythms, it is still unclear whether microglia are involved in the generation and light entrainment of circadian rhythms of behavior. Here, we report that microglial depletion does not alter behavioral circadian rhythms. We depleted ~ 95% of microglia in the mouse brain by PLX3397, a CSF1R inhibitor, and analyzed the effect on the spontaneous behaviors of mice. We found that neither the free-running period under constant darkness nor light entrainment under jet-lag circumstances were influenced by the ablation of microglia. Our results demonstrate that the circadian rhythms of locomotor activity, an important output of the circadian clock in the brain, are likely a phenomenon not produced by microglia. |
| first_indexed | 2024-04-09T18:50:16Z |
| format | Article |
| id | doaj.art-1c062324a0e649d79fe8540a08919737 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-04-09T18:50:16Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-1c062324a0e649d79fe8540a089197372023-04-09T11:30:04ZengBMCMolecular Brain1756-66062023-04-011611410.1186/s13041-023-01021-1Ablation of microglia does not alter circadian rhythm of locomotor activityFutaba Matsui0Sho T. Yamaguchi1Riho Kobayashi2Shiho Ito3Sakimi Nagashima4Zhiwen Zhou5Hiroaki Norimoto6Graduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityGraduate School of Medicine, Hokkaido UniversityAbstract Microglia, as macrophages in the brain, are responsible for immune responses and synaptic remodeling. Although the function of microglia is regulated by circadian rhythms, it is still unclear whether microglia are involved in the generation and light entrainment of circadian rhythms of behavior. Here, we report that microglial depletion does not alter behavioral circadian rhythms. We depleted ~ 95% of microglia in the mouse brain by PLX3397, a CSF1R inhibitor, and analyzed the effect on the spontaneous behaviors of mice. We found that neither the free-running period under constant darkness nor light entrainment under jet-lag circumstances were influenced by the ablation of microglia. Our results demonstrate that the circadian rhythms of locomotor activity, an important output of the circadian clock in the brain, are likely a phenomenon not produced by microglia.https://doi.org/10.1186/s13041-023-01021-1MicrogliaCircadian rhythmSuprachiasmatic nucleus |
| spellingShingle | Futaba Matsui Sho T. Yamaguchi Riho Kobayashi Shiho Ito Sakimi Nagashima Zhiwen Zhou Hiroaki Norimoto Ablation of microglia does not alter circadian rhythm of locomotor activity Molecular Brain Microglia Circadian rhythm Suprachiasmatic nucleus |
| title | Ablation of microglia does not alter circadian rhythm of locomotor activity |
| title_full | Ablation of microglia does not alter circadian rhythm of locomotor activity |
| title_fullStr | Ablation of microglia does not alter circadian rhythm of locomotor activity |
| title_full_unstemmed | Ablation of microglia does not alter circadian rhythm of locomotor activity |
| title_short | Ablation of microglia does not alter circadian rhythm of locomotor activity |
| title_sort | ablation of microglia does not alter circadian rhythm of locomotor activity |
| topic | Microglia Circadian rhythm Suprachiasmatic nucleus |
| url | https://doi.org/10.1186/s13041-023-01021-1 |
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