SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE
The article presents research materials on the selection of in vitro drought tolerant somatic tea clones (Sc-4, Sc-15, Sc-27, Sc-33) obtained by induction of hemogenesis from callus tissue. Osmotic stress was modeled by adding polyethylene glycol (PEG-30.0 g/l) to the growth medium. Changes in physi...
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| Format: | Article |
| Language: | Russian |
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Maykop State Technological University
2020-11-01
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| Series: | Новые технологии |
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| Online Access: | https://newtechology.mkgtu.ru/jour/article/view/397 |
| _version_ | 1797516391093370880 |
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| author | M. V. Gvasaliya |
| author_facet | M. V. Gvasaliya |
| author_sort | M. V. Gvasaliya |
| collection | DOAJ |
| description | The article presents research materials on the selection of in vitro drought tolerant somatic tea clones (Sc-4, Sc-15, Sc-27, Sc-33) obtained by induction of hemogenesis from callus tissue. Osmotic stress was modeled by adding polyethylene glycol (PEG-30.0 g/l) to the growth medium. Changes in physiological parameters during water deficiency were studied by the relative electrical conductivity and stability of cell membranes using a ST300C conductometer.The addition of PEG to the nutrient medium caused an increase in electrical conductivity in all studied somatic tea clones, with the exception of Sc-27, in which this indicator was lower compared to the control one.The stability of cell membranes in Sc-27 was higher compared to the control one. These data indicate a low level of damage to plant tissue. PCR analysis showed that the dehydrin 2 (DHN2) gene, one of the main genetic markers of drought response of plants, was expressed significantly higher in somaclone Sc-27. For all the parameters studied the Sc-27 somatic tea clone can be preliminarily selected as a drought tolerant genotype and can be used in a further breeding program. |
| first_indexed | 2024-03-10T07:01:35Z |
| format | Article |
| id | doaj.art-1c07593116a74e3593c095c35ef9679c |
| institution | Directory Open Access Journal |
| issn | 2072-0920 2713-0029 |
| language | Russian |
| last_indexed | 2024-03-10T07:01:35Z |
| publishDate | 2020-11-01 |
| publisher | Maykop State Technological University |
| record_format | Article |
| series | Новые технологии |
| spelling | doaj.art-1c07593116a74e3593c095c35ef9679c2023-11-22T16:04:54ZrusMaykop State Technological UniversityНовые технологии2072-09202713-00292020-11-010311412110.24411/2072-0920-2020-10313386SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTUREM. V. Gvasaliya0Federal State Budgetary Institution of Science (Federal Research Center of the Subtropical Scientific Center of the Russian Academy of Sciences)The article presents research materials on the selection of in vitro drought tolerant somatic tea clones (Sc-4, Sc-15, Sc-27, Sc-33) obtained by induction of hemogenesis from callus tissue. Osmotic stress was modeled by adding polyethylene glycol (PEG-30.0 g/l) to the growth medium. Changes in physiological parameters during water deficiency were studied by the relative electrical conductivity and stability of cell membranes using a ST300C conductometer.The addition of PEG to the nutrient medium caused an increase in electrical conductivity in all studied somatic tea clones, with the exception of Sc-27, in which this indicator was lower compared to the control one.The stability of cell membranes in Sc-27 was higher compared to the control one. These data indicate a low level of damage to plant tissue. PCR analysis showed that the dehydrin 2 (DHN2) gene, one of the main genetic markers of drought response of plants, was expressed significantly higher in somaclone Sc-27. For all the parameters studied the Sc-27 somatic tea clone can be preliminarily selected as a drought tolerant genotype and can be used in a further breeding program.https://newtechology.mkgtu.ru/jour/article/view/397tea somaclones (camellia sinensis (l.) kuntze)osmotic stresspolyethylene glycol (peg)relative conductivitystability of cell membranesgene expressiondehydrin 2 gene (dhn2) |
| spellingShingle | M. V. Gvasaliya SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE Новые технологии tea somaclones (camellia sinensis (l.) kuntze) osmotic stress polyethylene glycol (peg) relative conductivity stability of cell membranes gene expression dehydrin 2 gene (dhn2) |
| title | SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE |
| title_full | SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE |
| title_fullStr | SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE |
| title_full_unstemmed | SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE |
| title_short | SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE |
| title_sort | selection for dry resistance of somatic clones of tea plants camellia sinensis l o kuntze in in vitro culture |
| topic | tea somaclones (camellia sinensis (l.) kuntze) osmotic stress polyethylene glycol (peg) relative conductivity stability of cell membranes gene expression dehydrin 2 gene (dhn2) |
| url | https://newtechology.mkgtu.ru/jour/article/view/397 |
| work_keys_str_mv | AT mvgvasaliya selectionfordryresistanceofsomaticclonesofteaplantscamelliasinensislokuntzeininvitroculture |