Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus
The effects of temperature, pH, osmolarity and aeration on the growth and differentiation of a trypanosome ofthe subgenus Schizotrypanum isolatedfrom the bat Phyllostomus hastatus were studied. In general, the growth characteristics ofthe flagellate were similar to those of Trypanosoma (Schizotrypan...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1993-12-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821993000400005 |
| _version_ | 1818230002026545152 |
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| author | Sônia I. Hamanaka Artur da S. Pinto |
| author_facet | Sônia I. Hamanaka Artur da S. Pinto |
| author_sort | Sônia I. Hamanaka |
| collection | DOAJ |
| description | The effects of temperature, pH, osmolarity and aeration on the growth and differentiation of a trypanosome ofthe subgenus Schizotrypanum isolatedfrom the bat Phyllostomus hastatus were studied. In general, the growth characteristics ofthe flagellate were similar to those of Trypanosoma (Schizotrypanum) cruzi. However, the parasite did not growth at 33 or 37C. Increase in the osmolarity and aeration promoted growth at 33C. Significant metacyclogenesis was detected only in the growth condition where maximal growth occured (28C, pH 7.3, 380m0s/kg, in tissue cullure flasks), at the end ofthe exponential growth phase. The begining of the metacyclogenesis process was coincident with most glucose utilization and lowest pH. During metacyclogenesis both culture medium pH and osmolarity increased steadly.<br>Foram estudados os efeitos da temperatura, do pH, da osmolaridade e da areaçâo sobre o crescimento e a diferenciação de um tripanosoma do subgenêro Schizotrypanum, isolado do morcego Phyllostomus hastatus. Em geral, as características do crescimento do flagelado foram semelhantes àquelas do Trypanosoma (Schizotrypanum) cruzi. Entretanto, o parasita não de desenvolveu a 33 ou 37°C. O aumento na osmolaridade e areaçâo estimulou o crescimento a 33°C. Metaciclogênese sifhificativa foi detectada somente na condição de crescimento, onde ocorreu desenvolvimento máximo (28°C, pH 7.3, 320m0/kg H20, em frascos de cultura de tecido), no final da fase do crescimento exponencial. O início do processo de metaciclogênese coincidiu com maior utilização de glucose e menor pH. Durante a metaciclogênese, o pHdo meio de cultura e a osmolaridade aumentaram constantemente. |
| first_indexed | 2024-12-12T10:27:34Z |
| format | Article |
| id | doaj.art-1c08d6f448b34a00a31a36378e036e36 |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T10:27:34Z |
| publishDate | 1993-12-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-1c08d6f448b34a00a31a36378e036e362022-12-22T00:27:25ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98491993-12-01264225230Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatusSônia I. HamanakaArtur da S. PintoThe effects of temperature, pH, osmolarity and aeration on the growth and differentiation of a trypanosome ofthe subgenus Schizotrypanum isolatedfrom the bat Phyllostomus hastatus were studied. In general, the growth characteristics ofthe flagellate were similar to those of Trypanosoma (Schizotrypanum) cruzi. However, the parasite did not growth at 33 or 37C. Increase in the osmolarity and aeration promoted growth at 33C. Significant metacyclogenesis was detected only in the growth condition where maximal growth occured (28C, pH 7.3, 380m0s/kg, in tissue cullure flasks), at the end ofthe exponential growth phase. The begining of the metacyclogenesis process was coincident with most glucose utilization and lowest pH. During metacyclogenesis both culture medium pH and osmolarity increased steadly.<br>Foram estudados os efeitos da temperatura, do pH, da osmolaridade e da areaçâo sobre o crescimento e a diferenciação de um tripanosoma do subgenêro Schizotrypanum, isolado do morcego Phyllostomus hastatus. Em geral, as características do crescimento do flagelado foram semelhantes àquelas do Trypanosoma (Schizotrypanum) cruzi. Entretanto, o parasita não de desenvolveu a 33 ou 37°C. O aumento na osmolaridade e areaçâo estimulou o crescimento a 33°C. Metaciclogênese sifhificativa foi detectada somente na condição de crescimento, onde ocorreu desenvolvimento máximo (28°C, pH 7.3, 320m0/kg H20, em frascos de cultura de tecido), no final da fase do crescimento exponencial. O início do processo de metaciclogênese coincidiu com maior utilização de glucose e menor pH. Durante a metaciclogênese, o pHdo meio de cultura e a osmolaridade aumentaram constantemente.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821993000400005Schizotrypanum do morcego Phillostomus hastatusDesenvolvimentoDiferenciaçãoSchizotrypanum from Phyllostomus hastatusGrowthDifferentiation |
| spellingShingle | Sônia I. Hamanaka Artur da S. Pinto Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus Revista da Sociedade Brasileira de Medicina Tropical Schizotrypanum do morcego Phillostomus hastatus Desenvolvimento Diferenciação Schizotrypanum from Phyllostomus hastatus Growth Differentiation |
| title | Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus |
| title_full | Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus |
| title_fullStr | Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus |
| title_full_unstemmed | Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus |
| title_short | Growth and differentiation on a trypanosome of the subgenus Schizotrypanum from the bat Phyllostomus hastatus |
| title_sort | growth and differentiation on a trypanosome of the subgenus schizotrypanum from the bat phyllostomus hastatus |
| topic | Schizotrypanum do morcego Phillostomus hastatus Desenvolvimento Diferenciação Schizotrypanum from Phyllostomus hastatus Growth Differentiation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821993000400005 |
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