Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia
Abstract Background Chestnut seeds are important kinds of edible nuts rich in starch and protein. The characteristics and nutrient contents of chestnut have been found to show obvious metaxenia effects in previous studies. To improve the understanding of the effect of metaxenia on chestnut starch an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07923-5 |
| _version_ | 1818646507258118144 |
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| author | Shengxing Li Zhuogong Shi Qiurong Zhu Liang Tao Wenhui Liang Zhiheng Zhao |
| author_facet | Shengxing Li Zhuogong Shi Qiurong Zhu Liang Tao Wenhui Liang Zhiheng Zhao |
| author_sort | Shengxing Li |
| collection | DOAJ |
| description | Abstract Background Chestnut seeds are important kinds of edible nuts rich in starch and protein. The characteristics and nutrient contents of chestnut have been found to show obvious metaxenia effects in previous studies. To improve the understanding of the effect of metaxenia on chestnut starch and sucrose metabolism, this study used three varieties of chestnut, ‘Yongfeng 1’, ‘YongRen Zao’ and ‘Yimen 1’, as male parents to pollinate the female parent, ‘Yongfeng 1’, and investigated the mechanisms of starch and sucrose metabolism in three starch accumulation stages (70 (S1), 82 (S2), and 94 (S3) days after pollination, DAP) in chestnut seed kernels. Result Most carbohydrate metabolism genes were highly expressed in YFF (self-pollinated ‘Yongfeng 1’) in stage S2 and in YFR (‘Yongfeng 1’ × ‘Yongren Zao’) and YFM (‘Yongfeng 1’ × ‘Yimen 1’) in stage S3. In stage S3, hub genes encoding HSF_DNA-binding, ACT, Pkinase, and LIM proteins and four transcription factors were highly expressed, with YFF showing the highest expression, followed by YFR and YFM. In addition, transcriptome analysis of the kernels at 70, 82 and 94 DAP showed that the starch granule-bound starch synthase (EC 2.4.1.242) and ADP-glucose pyrophosphorylase (EC 2.7 .7.27) genes were actively expressed at 94 DAF. Chestnut seeds regulate the accumulation of soluble sugars, reducing sugars and starch by controlling glycosyl transferase and hydrolysis activity during development. Conclusion These results and resources have important guiding significance for further research on starch and sucrose metabolism and other types of metabolism related to chestnut metaxenia. |
| first_indexed | 2024-12-17T00:47:44Z |
| format | Article |
| id | doaj.art-1c0dbc9950244ff9b8b83862ff672f33 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T00:47:44Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-1c0dbc9950244ff9b8b83862ff672f332022-12-21T22:09:51ZengBMCBMC Genomics1471-21642021-08-0122111410.1186/s12864-021-07923-5Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut MetaxeniaShengxing Li0Zhuogong Shi1Qiurong Zhu2Liang Tao3Wenhui Liang4Zhiheng Zhao5Guanxi forestry research instituteGuanxi forestry research instituteGuanxi forestry research instituteGuanxi forestry research instituteGuanxi forestry research instituteGuanxi forestry research instituteAbstract Background Chestnut seeds are important kinds of edible nuts rich in starch and protein. The characteristics and nutrient contents of chestnut have been found to show obvious metaxenia effects in previous studies. To improve the understanding of the effect of metaxenia on chestnut starch and sucrose metabolism, this study used three varieties of chestnut, ‘Yongfeng 1’, ‘YongRen Zao’ and ‘Yimen 1’, as male parents to pollinate the female parent, ‘Yongfeng 1’, and investigated the mechanisms of starch and sucrose metabolism in three starch accumulation stages (70 (S1), 82 (S2), and 94 (S3) days after pollination, DAP) in chestnut seed kernels. Result Most carbohydrate metabolism genes were highly expressed in YFF (self-pollinated ‘Yongfeng 1’) in stage S2 and in YFR (‘Yongfeng 1’ × ‘Yongren Zao’) and YFM (‘Yongfeng 1’ × ‘Yimen 1’) in stage S3. In stage S3, hub genes encoding HSF_DNA-binding, ACT, Pkinase, and LIM proteins and four transcription factors were highly expressed, with YFF showing the highest expression, followed by YFR and YFM. In addition, transcriptome analysis of the kernels at 70, 82 and 94 DAP showed that the starch granule-bound starch synthase (EC 2.4.1.242) and ADP-glucose pyrophosphorylase (EC 2.7 .7.27) genes were actively expressed at 94 DAF. Chestnut seeds regulate the accumulation of soluble sugars, reducing sugars and starch by controlling glycosyl transferase and hydrolysis activity during development. Conclusion These results and resources have important guiding significance for further research on starch and sucrose metabolism and other types of metabolism related to chestnut metaxenia.https://doi.org/10.1186/s12864-021-07923-5ChestnutMetaxeniaDifferential gene expressionSeed developmentStarch synthesis |
| spellingShingle | Shengxing Li Zhuogong Shi Qiurong Zhu Liang Tao Wenhui Liang Zhiheng Zhao Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia BMC Genomics Chestnut Metaxenia Differential gene expression Seed development Starch synthesis |
| title | Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia |
| title_full | Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia |
| title_fullStr | Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia |
| title_full_unstemmed | Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia |
| title_short | Transcriptome sequencing and differential expression analysis of seed starch accumulation in Chinese chestnut Metaxenia |
| title_sort | transcriptome sequencing and differential expression analysis of seed starch accumulation in chinese chestnut metaxenia |
| topic | Chestnut Metaxenia Differential gene expression Seed development Starch synthesis |
| url | https://doi.org/10.1186/s12864-021-07923-5 |
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