Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host
This review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques. The advances in technology which now enables these light microscopy measurements are discussed with special emphasis on...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/2/223 |
| _version_ | 1797417056307511296 |
|---|---|
| author | Saveez Saffarian |
| author_facet | Saveez Saffarian |
| author_sort | Saveez Saffarian |
| collection | DOAJ |
| description | This review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques. The advances in technology which now enables these light microscopy measurements are discussed with special emphasis on live imaging approaches including Total Internal Reflection Fluorescence (TIRF), high-resolution light microscopy techniques including PALM and STORM and single molecule measurements, including Fluorescence Resonance Energy Transfer (FRET). The review concludes with a discussion on what new insights and understanding can be expected from these measurements. |
| first_indexed | 2024-03-09T06:13:24Z |
| format | Article |
| id | doaj.art-1c0f06e35d3c43ad95f807fe324b7580 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T06:13:24Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1c0f06e35d3c43ad95f807fe324b75802023-12-03T11:56:06ZengMDPI AGViruses1999-49152021-02-0113222310.3390/v13020223Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the HostSaveez Saffarian0Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USAThis review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques. The advances in technology which now enables these light microscopy measurements are discussed with special emphasis on live imaging approaches including Total Internal Reflection Fluorescence (TIRF), high-resolution light microscopy techniques including PALM and STORM and single molecule measurements, including Fluorescence Resonance Energy Transfer (FRET). The review concludes with a discussion on what new insights and understanding can be expected from these measurements.https://www.mdpi.com/1999-4915/13/2/223HIViPALMPALMSTORMSTEDTIRF |
| spellingShingle | Saveez Saffarian Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host Viruses HIV iPALM PALM STORM STED TIRF |
| title | Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host |
| title_full | Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host |
| title_fullStr | Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host |
| title_full_unstemmed | Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host |
| title_short | Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host |
| title_sort | application of advanced light microscopy to the study of hiv and its interactions with the host |
| topic | HIV iPALM PALM STORM STED TIRF |
| url | https://www.mdpi.com/1999-4915/13/2/223 |
| work_keys_str_mv | AT saveezsaffarian applicationofadvancedlightmicroscopytothestudyofhivanditsinteractionswiththehost |