Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA
<p>Four mitochondrial DNA segments, ND1, ND6, cyt <em>b</em> and D-loop, were analysed by polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) in 14 tench (<em>Tinca tinca</em> L.) populations located in Europe and Asia; also data on five Italia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2012-01-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/2370 |
| _version_ | 1818206323058147328 |
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| author | Liliana Di Stasio Claudio Lisa Petra Kersten Laura Gasco Klaus Kohlmann Rossella Lo Presti |
| author_facet | Liliana Di Stasio Claudio Lisa Petra Kersten Laura Gasco Klaus Kohlmann Rossella Lo Presti |
| author_sort | Liliana Di Stasio |
| collection | DOAJ |
| description | <p>Four mitochondrial DNA segments, ND1, ND6, cyt <em>b</em> and D-loop, were analysed by polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) in 14 tench (<em>Tinca tinca</em> L.) populations located in Europe and Asia; also data on five Italian populations previously analysed for the same mtDNA segments were included in the study. All the considered segments were polymorphic and originated a total of 9 composite haplotypes, which were clustered into two haplogroups, A and B, possibly corresponding to the Western and Eastern phylogroups previously described in tench. Nine out of 19 populations showed polymorphism, with haplotype diversity ranging from 0.246 to 0.643 and nucleotide diversity from 0.009 to 0.078. Seventy-five percent of the pairwise comparisons were significant, indicating a high between-population variability. The Neighbour-Joining tree revealed the presence of three clusters, including <em>pure</em>populations, with only A or B haplogroup, and <em>mixed</em>populations, with both haplogroups. The possibility of identifying populations with different haplotypes has practical implications for both conservation and supportive stocking.</p> |
| first_indexed | 2024-12-12T04:11:12Z |
| format | Article |
| id | doaj.art-1c11b49621b64e2abbf5e0f7cbd8bb16 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-12T04:11:12Z |
| publishDate | 2012-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-1c11b49621b64e2abbf5e0f7cbd8bb162022-12-22T00:38:37ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2012-01-01111e19e1910.4081/ijas.2012.e19Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNALiliana Di StasioClaudio LisaPetra KerstenLaura GascoKlaus KohlmannRossella Lo Presti<p>Four mitochondrial DNA segments, ND1, ND6, cyt <em>b</em> and D-loop, were analysed by polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) in 14 tench (<em>Tinca tinca</em> L.) populations located in Europe and Asia; also data on five Italian populations previously analysed for the same mtDNA segments were included in the study. All the considered segments were polymorphic and originated a total of 9 composite haplotypes, which were clustered into two haplogroups, A and B, possibly corresponding to the Western and Eastern phylogroups previously described in tench. Nine out of 19 populations showed polymorphism, with haplotype diversity ranging from 0.246 to 0.643 and nucleotide diversity from 0.009 to 0.078. Seventy-five percent of the pairwise comparisons were significant, indicating a high between-population variability. The Neighbour-Joining tree revealed the presence of three clusters, including <em>pure</em>populations, with only A or B haplogroup, and <em>mixed</em>populations, with both haplogroups. The possibility of identifying populations with different haplotypes has practical implications for both conservation and supportive stocking.</p>http://www.aspajournal.it/index.php/ijas/article/view/2370Tinca tinca, Mitochondrial DNA, RFLP, Genetic variability |
| spellingShingle | Liliana Di Stasio Claudio Lisa Petra Kersten Laura Gasco Klaus Kohlmann Rossella Lo Presti Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA Italian Journal of Animal Science Tinca tinca, Mitochondrial DNA, RFLP, Genetic variability |
| title | Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA |
| title_full | Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA |
| title_fullStr | Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA |
| title_full_unstemmed | Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA |
| title_short | Genetic variability in tench (Tinca tinca L.) as revealed by PCR-RFLP analysis of mitochondrial DNA |
| title_sort | genetic variability in tench tinca tinca l as revealed by pcr rflp analysis of mitochondrial dna |
| topic | Tinca tinca, Mitochondrial DNA, RFLP, Genetic variability |
| url | http://www.aspajournal.it/index.php/ijas/article/view/2370 |
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