Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究)
从生物硝化池污水水样中分离了硝化细菌DNA，以其为模板，针对其16SrRNA基因及23SrRNA基因而设计合成了两对引物并分别进行了PCR扩增，扩增产物的2%琼脂糖凝胶电泳结果显示，硝化细菌DNA模板得到了特异性扩增.参照分子量标准（Marker）的电泳结果，被扩增的DNA条带大小分别约为400bp和730bp.实验结果表明PCR 技术可用于污水中硝化细菌的快速检测....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
1999-06-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.1999.26.2.83-86 |
| _version_ | 1797236553138831360 |
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| author | MINGZhen-huan(明镇寰) YUEChun-mei(岳春梅) XUBing-sen(徐炳森) (黄朴) |
| author_facet | MINGZhen-huan(明镇寰) YUEChun-mei(岳春梅) XUBing-sen(徐炳森) (黄朴) |
| author_sort | MINGZhen-huan(明镇寰) |
| collection | DOAJ |
| description | 从生物硝化池污水水样中分离了硝化细菌DNA，以其为模板，针对其16SrRNA基因及23SrRNA基因而设计合成了两对引物并分别进行了PCR扩增，扩增产物的2%琼脂糖凝胶电泳结果显示，硝化细菌DNA模板得到了特异性扩增.参照分子量标准（Marker）的电泳结果，被扩增的DNA条带大小分别约为400bp和730bp.实验结果表明PCR 技术可用于污水中硝化细菌的快速检测. |
| first_indexed | 2024-04-24T17:05:41Z |
| format | Article |
| id | doaj.art-1c11e82def9649e3990eaa05f62d2c63 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:05:41Z |
| publishDate | 1999-06-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-1c11e82def9649e3990eaa05f62d2c632024-03-29T01:58:16ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94971999-06-012628386zjup/1008-9497.1999.26.2.83-86Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究)MINGZhen-huan(明镇寰)0YUEChun-mei(岳春梅)1XUBing-sen(徐炳森)2(黄朴)3College of Life Science, Zhejiang University, Hangzhou 310012, China(浙江大学西溪校区生命科学学院，浙江 杭州 310012)College of Life Science, Zhejiang University, Hangzhou 310012, China(浙江大学西溪校区生命科学学院，浙江 杭州 310012)College of Life Science, Zhejiang University, Hangzhou 310012, China(浙江大学西溪校区生命科学学院，浙江 杭州 310012)College of Life Science, Zhejiang University, Hangzhou 310012, China(浙江大学西溪校区生命科学学院，浙江 杭州 310012)从生物硝化池污水水样中分离了硝化细菌DNA，以其为模板，针对其16SrRNA基因及23SrRNA基因而设计合成了两对引物并分别进行了PCR扩增，扩增产物的2%琼脂糖凝胶电泳结果显示，硝化细菌DNA模板得到了特异性扩增.参照分子量标准（Marker）的电泳结果，被扩增的DNA条带大小分别约为400bp和730bp.实验结果表明PCR 技术可用于污水中硝化细菌的快速检测.https://doi.org/zjup/1008-9497.1999.26.2.83-86硝化细菌污水多聚酶链式反应 |
| spellingShingle | MINGZhen-huan(明镇寰) YUEChun-mei(岳春梅) XUBing-sen(徐炳森) (黄朴) Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) Zhejiang Daxue xuebao. Lixue ban 硝化细菌 污水 多聚酶链式反应 |
| title | Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) |
| title_full | Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) |
| title_fullStr | Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) |
| title_full_unstemmed | Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) |
| title_short | Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究) |
| title_sort | detection of nitrobacteria populations in wastewater of biological nitrification stage by polymerase chain reaction analysis 用pcr技术检测生物硝化池污水中硝化细菌 nitrobacteria 的研究 |
| topic | 硝化细菌 污水 多聚酶链式反应 |
| url | https://doi.org/zjup/1008-9497.1999.26.2.83-86 |
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