Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk
The aim of this study was to compare the sensitivity to selected antibiotics in staphylococci isolated from goat (n = 60), sheep (n = 60) and cow’s milk (n = 120). The individual milk samples were inoculated onto Blood agar cultivated at 36 °C/24 h.The isolated species of staphylococci were identifi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mendel University Press
2011-01-01
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| Series: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| Subjects: | |
| Online Access: | https://acta.mendelu.cz/59/3/0209/ |
| _version_ | 1818413843094700032 |
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| author | Marcela Vyletělová Oto Hanuš Renáta Karpíšková Zora Šťástková |
| author_facet | Marcela Vyletělová Oto Hanuš Renáta Karpíšková Zora Šťástková |
| author_sort | Marcela Vyletělová |
| collection | DOAJ |
| description | The aim of this study was to compare the sensitivity to selected antibiotics in staphylococci isolated from goat (n = 60), sheep (n = 60) and cow’s milk (n = 120). The individual milk samples were inoculated onto Blood agar cultivated at 36 °C/24 h.The isolated species of staphylococci were identified using biochemical tests, namely STAPHYtest and identification program TNW pro 6.5. We examined the sensitivity of strains to the spectrum of antibiotics, as follows: vancomycin (VA), amoxicillin/clavulanic acid (AMC), penicillin (P), rifampicin (RD), oxacillin (OX), tetracycline (TE), erythromycin (E), chloramphenicol (C), clindamycin (DA), gentamicin (CN), ciprofloxacin (CIP), teicoplanin (TEC), cefoxitin (FOX) and novobiocin (NOV). Altogether, 97 staphylococcal isolates were obtained; 70 from cow’s milk, 11 from goat’s milk and 16 from sheep’ milk. Staphylococcus aureus was the most frequent species in milk of all animal origin tested, was detected in 54 (45%) cow’s milk, 10 (17%) goat’s and 15 (25%) sheep’ milk samples. S. lentus was identified only in goat’s and sheep’ milk whereas in cow’s milk there were representation of staphylococcal species as follows: S. haemolyticus (n = 7), S. chromogenes (n = 2), S. warneri (n = 2), S. xylosus (n = 2), S. epidermidis (n = 2) and unclassified staphylococci (n = 1). The results of S. aureus sensitivity are similar for all tested antibiotics and for all monitored milk: No resistance to vancomycin, rifampicin, chloramphenicol and teicoplanin was recorded in obtained S. aureus isolates whereas the resistance to ciprofloxacin was found out most often. |
| first_indexed | 2024-12-14T11:09:38Z |
| format | Article |
| id | doaj.art-1c1470b2656f4e4dbdb39d069f2ec80a |
| institution | Directory Open Access Journal |
| issn | 1211-8516 2464-8310 |
| language | English |
| last_indexed | 2024-12-14T11:09:38Z |
| publishDate | 2011-01-01 |
| publisher | Mendel University Press |
| record_format | Article |
| series | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| spelling | doaj.art-1c1470b2656f4e4dbdb39d069f2ec80a2022-12-21T23:04:21ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102011-01-0159320921410.11118/actaun201159030209Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milkMarcela Vyletělová0Oto Hanuš1Renáta Karpíšková2Zora Šťástková3Výzkumný ústav pro chov skotu a Agrovýzkum Rapotín, s.r.o., Výzkumníků 267, 788 13 Vikýřovice, Česká republikaVýzkumný ústav pro chov skotu, Výzkumníků 267, 788 13 Vikýřovice, Česká republikaVeterinární a farmaceutická univerzita Brno, Palackého 1-3, 612 42 Brno, Česká republikaVeterinární a farmaceutická univerzita Brno, Palackého 1-3, 612 42 Brno, Česká republikaThe aim of this study was to compare the sensitivity to selected antibiotics in staphylococci isolated from goat (n = 60), sheep (n = 60) and cow’s milk (n = 120). The individual milk samples were inoculated onto Blood agar cultivated at 36 °C/24 h.The isolated species of staphylococci were identified using biochemical tests, namely STAPHYtest and identification program TNW pro 6.5. We examined the sensitivity of strains to the spectrum of antibiotics, as follows: vancomycin (VA), amoxicillin/clavulanic acid (AMC), penicillin (P), rifampicin (RD), oxacillin (OX), tetracycline (TE), erythromycin (E), chloramphenicol (C), clindamycin (DA), gentamicin (CN), ciprofloxacin (CIP), teicoplanin (TEC), cefoxitin (FOX) and novobiocin (NOV). Altogether, 97 staphylococcal isolates were obtained; 70 from cow’s milk, 11 from goat’s milk and 16 from sheep’ milk. Staphylococcus aureus was the most frequent species in milk of all animal origin tested, was detected in 54 (45%) cow’s milk, 10 (17%) goat’s and 15 (25%) sheep’ milk samples. S. lentus was identified only in goat’s and sheep’ milk whereas in cow’s milk there were representation of staphylococcal species as follows: S. haemolyticus (n = 7), S. chromogenes (n = 2), S. warneri (n = 2), S. xylosus (n = 2), S. epidermidis (n = 2) and unclassified staphylococci (n = 1). The results of S. aureus sensitivity are similar for all tested antibiotics and for all monitored milk: No resistance to vancomycin, rifampicin, chloramphenicol and teicoplanin was recorded in obtained S. aureus isolates whereas the resistance to ciprofloxacin was found out most often.https://acta.mendelu.cz/59/3/0209/milk<i>Staphylococcus</i> spp.antibiotics |
| spellingShingle | Marcela Vyletělová Oto Hanuš Renáta Karpíšková Zora Šťástková Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis milk <i>Staphylococcus</i> spp. antibiotics |
| title | Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk |
| title_full | Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk |
| title_fullStr | Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk |
| title_full_unstemmed | Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk |
| title_short | Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk |
| title_sort | occurrence and antimicrobial sensitivity in staphylococci isolated from goat sheep and cow s milk |
| topic | milk <i>Staphylococcus</i> spp. antibiotics |
| url | https://acta.mendelu.cz/59/3/0209/ |
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