Characterization of denaturation and renaturation of DNA for DNA hybridization
Objectives The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. Methods A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment...
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| Format: | Article |
| Language: | English |
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Korean Society of Environmental Health and Toxicology
2014-09-01
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| Series: | Environmental Health and Toxicology |
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| Online Access: | http://www.e-eht.org/upload/pdf/eht-29-e2014007.pdf |
| _version_ | 1818732917056077824 |
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| author | Xiaofang Wang Hyun Jeong Lim Ahjeong Son |
| author_facet | Xiaofang Wang Hyun Jeong Lim Ahjeong Son |
| author_sort | Xiaofang Wang |
| collection | DOAJ |
| description | Objectives The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. Methods A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was determined. DNA renaturation tendency was also investigated for the suggested denaturation method. Results Heating, beads mill, and sonication bath did not show any denaturation for 30 minutes. However probe sonication fully denatured DNA in 5 minutes. 1 mol/L sodium hydroxide (alkaline treatment) and 60% dimethyl sulfoxide (DMSO) treatment fully denatured DNA in 2-5 minutes. Conclusions Among all the physical methods applied, the direct probe sonication was the most effective way to denature the DNA fragments. Among chemical methods, 60% DMSO was the most adequate denaturation method since it does not cause full renaturation during DNA hybridization. |
| first_indexed | 2024-12-17T23:41:11Z |
| format | Article |
| id | doaj.art-1c1751c1114c4cbcba1bf837f0b7ab41 |
| institution | Directory Open Access Journal |
| issn | 2233-6567 |
| language | English |
| last_indexed | 2024-12-17T23:41:11Z |
| publishDate | 2014-09-01 |
| publisher | Korean Society of Environmental Health and Toxicology |
| record_format | Article |
| series | Environmental Health and Toxicology |
| spelling | doaj.art-1c1751c1114c4cbcba1bf837f0b7ab412022-12-21T21:28:26ZengKorean Society of Environmental Health and ToxicologyEnvironmental Health and Toxicology2233-65672014-09-012910.5620/eht.2014.29.e201400770Characterization of denaturation and renaturation of DNA for DNA hybridizationXiaofang Wang0Hyun Jeong Lim1Ahjeong Son2 Department of Civil Engineering, Auburn University, Auburn, AL, USA Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Korea Department of Environmental Science and Engineering, Ewha Womans University, Seoul, KoreaObjectives The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. Methods A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was determined. DNA renaturation tendency was also investigated for the suggested denaturation method. Results Heating, beads mill, and sonication bath did not show any denaturation for 30 minutes. However probe sonication fully denatured DNA in 5 minutes. 1 mol/L sodium hydroxide (alkaline treatment) and 60% dimethyl sulfoxide (DMSO) treatment fully denatured DNA in 2-5 minutes. Conclusions Among all the physical methods applied, the direct probe sonication was the most effective way to denature the DNA fragments. Among chemical methods, 60% DMSO was the most adequate denaturation method since it does not cause full renaturation during DNA hybridization.http://www.e-eht.org/upload/pdf/eht-29-e2014007.pdfBeads millDimethyl sulfoxideDNA denaturationDNA renaturationHeatingSonication |
| spellingShingle | Xiaofang Wang Hyun Jeong Lim Ahjeong Son Characterization of denaturation and renaturation of DNA for DNA hybridization Environmental Health and Toxicology Beads mill Dimethyl sulfoxide DNA denaturation DNA renaturation Heating Sonication |
| title | Characterization of denaturation and renaturation of DNA for DNA hybridization |
| title_full | Characterization of denaturation and renaturation of DNA for DNA hybridization |
| title_fullStr | Characterization of denaturation and renaturation of DNA for DNA hybridization |
| title_full_unstemmed | Characterization of denaturation and renaturation of DNA for DNA hybridization |
| title_short | Characterization of denaturation and renaturation of DNA for DNA hybridization |
| title_sort | characterization of denaturation and renaturation of dna for dna hybridization |
| topic | Beads mill Dimethyl sulfoxide DNA denaturation DNA renaturation Heating Sonication |
| url | http://www.e-eht.org/upload/pdf/eht-29-e2014007.pdf |
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