| Summary: | Herein, the Co<sub>3</sub>O<sub>4</sub>/NiCo<sub>2</sub>O<sub>4</sub> nanocomposite has been prepared as a novel electrochemical sensor to accurately detect hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and glucose. ZIF-67 is a metal-organic framework (MOF) with Co as the center metal ion. Co<sub>3</sub>O<sub>4</sub> can be obtained by calcination of ZIF-67 at 700 °C, which can retain the structure of ZIF-67. The hollow Co<sub>3</sub>O<sub>4</sub> nanocrystal was synthesized based on a calcination process of ZIF-67. This open structure can promote the whole Co<sub>3</sub>O<sub>4</sub>/NiCo<sub>2</sub>O<sub>4</sub> nanocomposite larger accessible surface area and reactive sites. Co<sub>3</sub>O<sub>4</sub> has good electrocatalytic performance, which has been applied in many fields. Moreover, H<sub>2</sub>O<sub>2</sub> and dopamine sensing tests indicate that the as-prepared non-enzymatic electrochemical biosensor has good detection properties. The testing results indicate the as-prepared biosensor has a wide detection range, low detection limit, high selectivity, and long-term stability. These testing results suggest the potential application in food security, biomedicine, environmental detection, and pharmaceutical analysis.
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