Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L.
The aim of the present study was to obtain, characterize, and evaluate the antioxidant potential of some extracts obtained from the bark of <i>Betula alba</i> var. <i>pendula</i> Roth., the root of <i>Glycyrrhiza glabra</i> L., and the green herb of the <i>A...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/13/2510 |
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| author | Adelina Ghica Veronica Drumea Alina Moroșan Dan Eduard Mihaiescu Liliana Costea Emanuela Alice Luță Dragos Paul Mihai Dalila Teodora Balaci Ancuța Cătălina Fița Octavian Tudorel Olaru Rica Boscencu Cerasela Elena Gîrd |
| author_facet | Adelina Ghica Veronica Drumea Alina Moroșan Dan Eduard Mihaiescu Liliana Costea Emanuela Alice Luță Dragos Paul Mihai Dalila Teodora Balaci Ancuța Cătălina Fița Octavian Tudorel Olaru Rica Boscencu Cerasela Elena Gîrd |
| author_sort | Adelina Ghica |
| collection | DOAJ |
| description | The aim of the present study was to obtain, characterize, and evaluate the antioxidant potential of some extracts obtained from the bark of <i>Betula alba</i> var. <i>pendula</i> Roth., the root of <i>Glycyrrhiza glabra</i> L., and the green herb of the <i>Avena sativa</i>. The results revealed that the lowest IC50 value, determined by all three methods, was obtained for <i>Betulae extractum</i> (BE) (73.6 µg/mL—DPPH method, 11.2 µg/mL—ABTS method, and 58.7 µg/mL—FRAP method), followed by <i>Liquiritiae extractum</i> (LE) (805.6 µg/mL, 92.1 µg/mL, and 722 µg/mL) and <i>Avenae extractum</i> (1.13 mg/mL—DPPH method, 99.7 µg/mL—ABTS method, and 135.1 µg/mL—FRAP method). These results correlate with total polyphenols content (expressed in g tannic acid/100 g dry extract), with BE having more polyphenols than LE and AE (47.96 ± 9.7083 for BE, compared with 9.31 ± 0.9913 for LE and 40.55 ± 6.3715 for AE). The total flavonoid content (expressed as g rutoside/100 g dry extract) is similar for BE and LE (3.75 ± 0.3140 and 3.44 ± 0.3037) and smaller for AE (1.95 ± 0.0526). Therefore, <i>Betulae extractum</i> has the strongest antioxidant action, with an IC50 value very close to the standard used as a reference (ascorbic acid—16.5 μg/mL solution). The FT-ICR-MS analysis confirmed the presence of the major compounds in all three extracts. The antioxidant properties of the studied extracts were further supported by molecular docking experiments that revealed the potential of the analyzed phytochemicals to act as both noncovalent and covalent activators of the Nrf2 signaling pathway, with promising benefits in treating various skin disorders. |
| first_indexed | 2024-03-11T01:31:02Z |
| format | Article |
| id | doaj.art-1c21169de2784dcba03df1134ef20a23 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T01:31:02Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-1c21169de2784dcba03df1134ef20a232023-11-18T17:18:29ZengMDPI AGPlants2223-77472023-06-011213251010.3390/plants12132510Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L.Adelina Ghica0Veronica Drumea1Alina Moroșan2Dan Eduard Mihaiescu3Liliana Costea4Emanuela Alice Luță5Dragos Paul Mihai6Dalila Teodora Balaci7Ancuța Cătălina Fița8Octavian Tudorel Olaru9Rica Boscencu10Cerasela Elena Gîrd11Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaBiotehnos SA, Gorunului Street No. 3-5, 075100 Otopeni, RomaniaDepartment of Organic Chemistry “Costin Nenițescu”, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, 011061 Bucharest, RomaniaDepartment of Organic Chemistry “Costin Nenițescu”, Faculty of Applied Chemistry and Materials Science, University POLITEHNICA of Bucharest, 011061 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaFaculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, RomaniaThe aim of the present study was to obtain, characterize, and evaluate the antioxidant potential of some extracts obtained from the bark of <i>Betula alba</i> var. <i>pendula</i> Roth., the root of <i>Glycyrrhiza glabra</i> L., and the green herb of the <i>Avena sativa</i>. The results revealed that the lowest IC50 value, determined by all three methods, was obtained for <i>Betulae extractum</i> (BE) (73.6 µg/mL—DPPH method, 11.2 µg/mL—ABTS method, and 58.7 µg/mL—FRAP method), followed by <i>Liquiritiae extractum</i> (LE) (805.6 µg/mL, 92.1 µg/mL, and 722 µg/mL) and <i>Avenae extractum</i> (1.13 mg/mL—DPPH method, 99.7 µg/mL—ABTS method, and 135.1 µg/mL—FRAP method). These results correlate with total polyphenols content (expressed in g tannic acid/100 g dry extract), with BE having more polyphenols than LE and AE (47.96 ± 9.7083 for BE, compared with 9.31 ± 0.9913 for LE and 40.55 ± 6.3715 for AE). The total flavonoid content (expressed as g rutoside/100 g dry extract) is similar for BE and LE (3.75 ± 0.3140 and 3.44 ± 0.3037) and smaller for AE (1.95 ± 0.0526). Therefore, <i>Betulae extractum</i> has the strongest antioxidant action, with an IC50 value very close to the standard used as a reference (ascorbic acid—16.5 μg/mL solution). The FT-ICR-MS analysis confirmed the presence of the major compounds in all three extracts. The antioxidant properties of the studied extracts were further supported by molecular docking experiments that revealed the potential of the analyzed phytochemicals to act as both noncovalent and covalent activators of the Nrf2 signaling pathway, with promising benefits in treating various skin disorders.https://www.mdpi.com/2223-7747/12/13/2510phytochemical screeningradical scavenging activityFT-ICR-MSmolecular formulamolecular dockingKeap1-Nrf2 pathway |
| spellingShingle | Adelina Ghica Veronica Drumea Alina Moroșan Dan Eduard Mihaiescu Liliana Costea Emanuela Alice Luță Dragos Paul Mihai Dalila Teodora Balaci Ancuța Cătălina Fița Octavian Tudorel Olaru Rica Boscencu Cerasela Elena Gîrd Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. Plants phytochemical screening radical scavenging activity FT-ICR-MS molecular formula molecular docking Keap1-Nrf2 pathway |
| title | Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. |
| title_full | Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. |
| title_fullStr | Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. |
| title_full_unstemmed | Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. |
| title_short | Phytochemical Screening and Antioxidant Potential of Selected Extracts from <i>Betula alba</i> var. <i>pendula</i> Roth., <i>Glycyrrhiza glabra</i> L., and <i>Avena sativa</i> L. |
| title_sort | phytochemical screening and antioxidant potential of selected extracts from i betula alba i var i pendula i roth i glycyrrhiza glabra i l and i avena sativa i l |
| topic | phytochemical screening radical scavenging activity FT-ICR-MS molecular formula molecular docking Keap1-Nrf2 pathway |
| url | https://www.mdpi.com/2223-7747/12/13/2510 |
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