Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells
Abstract Purpose Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2023-01-01
|
| Series: | Discover Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s12672-023-00617-2 |
| _version_ | 1811175793120772096 |
|---|---|
| author | Motoki Ono Tsutomu Miyamoto Chiho Fuseya Ryoichi Asaka Hirofumi Ando Yasuhiro Tanaka Manaka Shinagawa Yusuke Yokokawa Hodaka Takeuchi Akiko Horiuchi Tanri Shiozawa |
| author_facet | Motoki Ono Tsutomu Miyamoto Chiho Fuseya Ryoichi Asaka Hirofumi Ando Yasuhiro Tanaka Manaka Shinagawa Yusuke Yokokawa Hodaka Takeuchi Akiko Horiuchi Tanri Shiozawa |
| author_sort | Motoki Ono |
| collection | DOAJ |
| description | Abstract Purpose Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated the anti-tumor effect of 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC), a major chemical compound of Wasabi, against various EMC cell lines in vitro and in vivo. Methods The effect of 6-MITC on cell viability was measured by the WST-1 assay in EMC and HUVEC cells. The impact of 6-MITC oral administration in nude mice was measured to assess the growth of the EMC xenograft and natural killer (NK) cell activity in the spleen. Results The addition of 6-MITC suppressed the proliferation of EMC cells (Ishikawa, HEC265, HEC108, KLE, and HEC1B) dose-dependently, but not HUVEC cells. 6-MITC (5 µM) enhanced the cisplatin sensitivity of EMC cells. 6-MITC induced apoptosis in a dose-dependent fashion in EMC cells other than HEC1B cells and was associated with increased expression of cleaved-caspase3 and decreased expression of BCL2. Oral administration of 6-MITC (2 and 4 µmol/kg) to Ishikawa and HEC1B xenografting mice resulted in a reduced tumor volume compared with the control (P < 0.05, 4 µmol/kg). Immunohistochemical staining of resected tumors revealed increased expression of Ki-67 and reduced cleaved-caspase3. Furthermore, 6-MITC treatment enhanced NK cell activity, especially when administered before tumor xenografting. Conclusion These results indicate that 6-MITC has a marked anti-tumor effect against EMC cells and a novel effect to enhance NK cell activity. These effects suggest the therapeutic potential of 6-MITC. |
| first_indexed | 2024-04-10T19:41:38Z |
| format | Article |
| id | doaj.art-1c218b32f2b841c784579fb358abd77e |
| institution | Directory Open Access Journal |
| issn | 2730-6011 |
| language | English |
| last_indexed | 2024-04-10T19:41:38Z |
| publishDate | 2023-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj.art-1c218b32f2b841c784579fb358abd77e2023-01-29T12:15:54ZengSpringerDiscover Oncology2730-60112023-01-0114111210.1007/s12672-023-00617-2Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cellsMotoki Ono0Tsutomu Miyamoto1Chiho Fuseya2Ryoichi Asaka3Hirofumi Ando4Yasuhiro Tanaka5Manaka Shinagawa6Yusuke Yokokawa7Hodaka Takeuchi8Akiko Horiuchi9Tanri Shiozawa10Department of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineHoriuchi Ladies ClinicDepartment of Obstetrics and Gynecology, Shinshu University School of MedicineAbstract Purpose Wasabi is a traditional plant seasoning with an anti-septic function. Recent studies revealed several functions of Wasabi, such as anti-inflammation; however, the anti-tumor effect against endometrial carcinoma (EMC) cells has not been examined. In the present study, we investigated the anti-tumor effect of 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC), a major chemical compound of Wasabi, against various EMC cell lines in vitro and in vivo. Methods The effect of 6-MITC on cell viability was measured by the WST-1 assay in EMC and HUVEC cells. The impact of 6-MITC oral administration in nude mice was measured to assess the growth of the EMC xenograft and natural killer (NK) cell activity in the spleen. Results The addition of 6-MITC suppressed the proliferation of EMC cells (Ishikawa, HEC265, HEC108, KLE, and HEC1B) dose-dependently, but not HUVEC cells. 6-MITC (5 µM) enhanced the cisplatin sensitivity of EMC cells. 6-MITC induced apoptosis in a dose-dependent fashion in EMC cells other than HEC1B cells and was associated with increased expression of cleaved-caspase3 and decreased expression of BCL2. Oral administration of 6-MITC (2 and 4 µmol/kg) to Ishikawa and HEC1B xenografting mice resulted in a reduced tumor volume compared with the control (P < 0.05, 4 µmol/kg). Immunohistochemical staining of resected tumors revealed increased expression of Ki-67 and reduced cleaved-caspase3. Furthermore, 6-MITC treatment enhanced NK cell activity, especially when administered before tumor xenografting. Conclusion These results indicate that 6-MITC has a marked anti-tumor effect against EMC cells and a novel effect to enhance NK cell activity. These effects suggest the therapeutic potential of 6-MITC.https://doi.org/10.1007/s12672-023-00617-26-(Methylsulfinyl) hexyl isothiocyanateWasabiEndometrial carcinomaAnti-tumor effectNatural killer cell activityApoptosis |
| spellingShingle | Motoki Ono Tsutomu Miyamoto Chiho Fuseya Ryoichi Asaka Hirofumi Ando Yasuhiro Tanaka Manaka Shinagawa Yusuke Yokokawa Hodaka Takeuchi Akiko Horiuchi Tanri Shiozawa Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells Discover Oncology 6-(Methylsulfinyl) hexyl isothiocyanate Wasabi Endometrial carcinoma Anti-tumor effect Natural killer cell activity Apoptosis |
| title | Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells |
| title_full | Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells |
| title_fullStr | Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells |
| title_full_unstemmed | Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells |
| title_short | Anti-tumor effect of Wasabi component, 6-(methylsulfinyl) hexyl isothiocyanate, against endometrial carcinoma cells |
| title_sort | anti tumor effect of wasabi component 6 methylsulfinyl hexyl isothiocyanate against endometrial carcinoma cells |
| topic | 6-(Methylsulfinyl) hexyl isothiocyanate Wasabi Endometrial carcinoma Anti-tumor effect Natural killer cell activity Apoptosis |
| url | https://doi.org/10.1007/s12672-023-00617-2 |
| work_keys_str_mv | AT motokiono antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT tsutomumiyamoto antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT chihofuseya antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT ryoichiasaka antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT hirofumiando antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT yasuhirotanaka antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT manakashinagawa antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT yusukeyokokawa antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT hodakatakeuchi antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT akikohoriuchi antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells AT tanrishiozawa antitumoreffectofwasabicomponent6methylsulfinylhexylisothiocyanateagainstendometrialcarcinomacells |