Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells
Abstract Although mitochondria are widely studied organelles, the recent interest in the role of mitochondrial small noncoding RNAs (sncRNAs), miRNAs, and more recently, piRNAs, is providing new functional perspectives in germ cell development and differentiation. piRNAs (PIWI-interacting RNAs) are...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14414-4 |
| _version_ | 1818545866376478720 |
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| author | Odei Barreñada Eduardo Larriba Daniel Fernández-Pérez Miguel Ángel Brieño-Enríquez Jesús del Mazo Martínez |
| author_facet | Odei Barreñada Eduardo Larriba Daniel Fernández-Pérez Miguel Ángel Brieño-Enríquez Jesús del Mazo Martínez |
| author_sort | Odei Barreñada |
| collection | DOAJ |
| description | Abstract Although mitochondria are widely studied organelles, the recent interest in the role of mitochondrial small noncoding RNAs (sncRNAs), miRNAs, and more recently, piRNAs, is providing new functional perspectives in germ cell development and differentiation. piRNAs (PIWI-interacting RNAs) are single-stranded sncRNAs of mostly about 20–35 nucleotides, generated from the processing of pre-piRNAs. We leverage next-generation sequencing data obtained from mouse primordial germ cells and somatic cells purified from early-differentiating embryonic ovaries and testis from 11.5 to 13.5 days postcoitum. Using bioinformatic tools, we elucidate (i) the origins of piRNAs as transcribed from mitochondrial DNA fragments inserted in the nucleus or from the mitochondrial genome; (ii) their levels of expression; and (iii) their potential roles, as well as their association with genomic regions encoding other sncRNAs (such as tRNAs and rRNAs) and the mitochondrial regulatory region (D-loop). Finally, our results suggest how nucleo-mitochondrial communication, both anterograde and retrograde signaling, may be mediated by mitochondria-associated piRNAs. |
| first_indexed | 2024-12-12T07:45:35Z |
| format | Article |
| id | doaj.art-1c2241e016354613b932d9f476181f80 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T07:45:35Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1c2241e016354613b932d9f476181f802022-12-22T00:32:36ZengNature PortfolioScientific Reports2045-23222022-06-0112111410.1038/s41598-022-14414-4Unraveling mitochondrial piRNAs in mouse embryonic gonadal cellsOdei Barreñada0Eduardo Larriba1Daniel Fernández-Pérez2Miguel Ángel Brieño-Enríquez3Jesús del Mazo Martínez4Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas C.I.B. (CSIC)Institute of Bioengineering, University “Miguel Hernández”Department of Experimental Oncology, IEO European Institute of Oncology–IRCCSMagee-Womens Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences. School of Medicine, University of PittsburghDepartment of Cellular and Molecular Biology, Centro de Investigaciones Biológicas Margarita Salas C.I.B. (CSIC)Abstract Although mitochondria are widely studied organelles, the recent interest in the role of mitochondrial small noncoding RNAs (sncRNAs), miRNAs, and more recently, piRNAs, is providing new functional perspectives in germ cell development and differentiation. piRNAs (PIWI-interacting RNAs) are single-stranded sncRNAs of mostly about 20–35 nucleotides, generated from the processing of pre-piRNAs. We leverage next-generation sequencing data obtained from mouse primordial germ cells and somatic cells purified from early-differentiating embryonic ovaries and testis from 11.5 to 13.5 days postcoitum. Using bioinformatic tools, we elucidate (i) the origins of piRNAs as transcribed from mitochondrial DNA fragments inserted in the nucleus or from the mitochondrial genome; (ii) their levels of expression; and (iii) their potential roles, as well as their association with genomic regions encoding other sncRNAs (such as tRNAs and rRNAs) and the mitochondrial regulatory region (D-loop). Finally, our results suggest how nucleo-mitochondrial communication, both anterograde and retrograde signaling, may be mediated by mitochondria-associated piRNAs.https://doi.org/10.1038/s41598-022-14414-4 |
| spellingShingle | Odei Barreñada Eduardo Larriba Daniel Fernández-Pérez Miguel Ángel Brieño-Enríquez Jesús del Mazo Martínez Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells Scientific Reports |
| title | Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells |
| title_full | Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells |
| title_fullStr | Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells |
| title_full_unstemmed | Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells |
| title_short | Unraveling mitochondrial piRNAs in mouse embryonic gonadal cells |
| title_sort | unraveling mitochondrial pirnas in mouse embryonic gonadal cells |
| url | https://doi.org/10.1038/s41598-022-14414-4 |
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