The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation
We investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of <i>Lemna minor</i> plants to antibiotics. For our investigation, we used specific inhibitors of these two H<sub>2</su...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/151 |
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| author | Marcelo Pedrosa Gomes Rafael Shinji Akiyama Kitamura Raizza Zorman Marques Marcello Locatelli Barbato Marcel Zámocký |
| author_facet | Marcelo Pedrosa Gomes Rafael Shinji Akiyama Kitamura Raizza Zorman Marques Marcello Locatelli Barbato Marcel Zámocký |
| author_sort | Marcelo Pedrosa Gomes |
| collection | DOAJ |
| description | We investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of <i>Lemna minor</i> plants to antibiotics. For our investigation, we used specific inhibitors of these two H<sub>2</sub>O<sub>2</sub>-scavenging enzymes (<i>p</i>-aminophenol, 3-amino,1,2,4-triazole, and salicylic acid). APX activity was central for the tolerance of this aquatic plant to amoxicillin (AMX), whereas CAT activity was important for avoiding oxidative damage when exposed to ciprofloxacin (CIP). Both monitored enzymes had important roles in the tolerance of <i>Lemna minor</i> to erythromycin (ERY). The use of molecular kinetic approaches to detect and increase APX and/or CAT scavenging activities could enhance tolerance, and, therefore, improve the use of <i>L. minor</i> plants to reclaim antibiotics from water bodies. |
| first_indexed | 2024-03-10T01:59:32Z |
| format | Article |
| id | doaj.art-1c2526bbc6594584897f8cdae2456d24 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T01:59:32Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1c2526bbc6594584897f8cdae2456d242023-11-23T12:48:09ZengMDPI AGAntioxidants2076-39212022-01-0111115110.3390/antiox11010151The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for PhytoremediationMarcelo Pedrosa Gomes0Rafael Shinji Akiyama Kitamura1Raizza Zorman Marques2Marcello Locatelli Barbato3Marcel Zámocký4Laboratório de Fisiologia de Plantas sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Avenida Coronel Francisco H. dos Santos, 100, Centro Politécnico Jardim das Américas, C.P. 19031, Curitiba 81531-980, BrazilLaboratório de Fisiologia de Plantas sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Avenida Coronel Francisco H. dos Santos, 100, Centro Politécnico Jardim das Américas, C.P. 19031, Curitiba 81531-980, BrazilLaboratório de Fisiologia de Plantas sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Avenida Coronel Francisco H. dos Santos, 100, Centro Politécnico Jardim das Américas, C.P. 19031, Curitiba 81531-980, BrazilLaboratório de Fisiologia de Plantas sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Avenida Coronel Francisco H. dos Santos, 100, Centro Politécnico Jardim das Américas, C.P. 19031, Curitiba 81531-980, BrazilLaboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551 Bratislava, SlovakiaWe investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of <i>Lemna minor</i> plants to antibiotics. For our investigation, we used specific inhibitors of these two H<sub>2</sub>O<sub>2</sub>-scavenging enzymes (<i>p</i>-aminophenol, 3-amino,1,2,4-triazole, and salicylic acid). APX activity was central for the tolerance of this aquatic plant to amoxicillin (AMX), whereas CAT activity was important for avoiding oxidative damage when exposed to ciprofloxacin (CIP). Both monitored enzymes had important roles in the tolerance of <i>Lemna minor</i> to erythromycin (ERY). The use of molecular kinetic approaches to detect and increase APX and/or CAT scavenging activities could enhance tolerance, and, therefore, improve the use of <i>L. minor</i> plants to reclaim antibiotics from water bodies.https://www.mdpi.com/2076-3921/11/1/151peroxidasecatalaseamoxicillinciprofloxacinerythromycinperoxidase inhibitors |
| spellingShingle | Marcelo Pedrosa Gomes Rafael Shinji Akiyama Kitamura Raizza Zorman Marques Marcello Locatelli Barbato Marcel Zámocký The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation Antioxidants peroxidase catalase amoxicillin ciprofloxacin erythromycin peroxidase inhibitors |
| title | The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation |
| title_full | The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation |
| title_fullStr | The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation |
| title_full_unstemmed | The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation |
| title_short | The Role of H<sub>2</sub>O<sub>2</sub>-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of <i>Lemna minor</i> to Antibiotics: Implications for Phytoremediation |
| title_sort | role of h sub 2 sub o sub 2 sub scavenging enzymes ascorbate peroxidase and catalase in the tolerance of i lemna minor i to antibiotics implications for phytoremediation |
| topic | peroxidase catalase amoxicillin ciprofloxacin erythromycin peroxidase inhibitors |
| url | https://www.mdpi.com/2076-3921/11/1/151 |
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