The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
The epigenetic landscape of a cell frequently changes in response to fluctuations in nutrient levels, but the mechanistic link is not well understood. In fission yeast, the JmjC domain protein Epe1 is critical for maintaining the heterochromatin landscape. While loss of Epe1 results in heterochromat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-02-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010049 |
| _version_ | 1811246218290921472 |
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| author | Kehan Bao Chun-Min Shan Xiao Chen Gulzhan Raiymbek Jeremy G Monroe Yimeng Fang Takenori Toda Kristin S Koutmou Kaushik Ragunathan Chao Lu Luke E Berchowitz Songtao Jia |
| author_facet | Kehan Bao Chun-Min Shan Xiao Chen Gulzhan Raiymbek Jeremy G Monroe Yimeng Fang Takenori Toda Kristin S Koutmou Kaushik Ragunathan Chao Lu Luke E Berchowitz Songtao Jia |
| author_sort | Kehan Bao |
| collection | DOAJ |
| description | The epigenetic landscape of a cell frequently changes in response to fluctuations in nutrient levels, but the mechanistic link is not well understood. In fission yeast, the JmjC domain protein Epe1 is critical for maintaining the heterochromatin landscape. While loss of Epe1 results in heterochromatin expansion, overexpression of Epe1 leads to defective heterochromatin. Through a genetic screen, we found that mutations in genes of the cAMP signaling pathway suppress the heterochromatin defects associated with Epe1 overexpression. We further demonstrated that the activation of Pka1, the downstream effector of cAMP signaling, is required for the efficient translation of epe1+ mRNA to maintain Epe1 overexpression. Moreover, inactivation of the cAMP-signaling pathway, either through genetic mutations or glucose deprivation, leads to the reduction of endogenous Epe1 and corresponding heterochromatin changes. These results reveal the mechanism by which the cAMP signaling pathway regulates heterochromatin landscape in fission yeast. |
| first_indexed | 2024-04-12T14:50:25Z |
| format | Article |
| id | doaj.art-1c262db5a8464ee0a433fff8500f564e |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T14:50:25Z |
| publishDate | 2022-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-1c262db5a8464ee0a433fff8500f564e2022-12-22T03:28:28ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-02-01182e101004910.1371/journal.pgen.1010049The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.Kehan BaoChun-Min ShanXiao ChenGulzhan RaiymbekJeremy G MonroeYimeng FangTakenori TodaKristin S KoutmouKaushik RagunathanChao LuLuke E BerchowitzSongtao JiaThe epigenetic landscape of a cell frequently changes in response to fluctuations in nutrient levels, but the mechanistic link is not well understood. In fission yeast, the JmjC domain protein Epe1 is critical for maintaining the heterochromatin landscape. While loss of Epe1 results in heterochromatin expansion, overexpression of Epe1 leads to defective heterochromatin. Through a genetic screen, we found that mutations in genes of the cAMP signaling pathway suppress the heterochromatin defects associated with Epe1 overexpression. We further demonstrated that the activation of Pka1, the downstream effector of cAMP signaling, is required for the efficient translation of epe1+ mRNA to maintain Epe1 overexpression. Moreover, inactivation of the cAMP-signaling pathway, either through genetic mutations or glucose deprivation, leads to the reduction of endogenous Epe1 and corresponding heterochromatin changes. These results reveal the mechanism by which the cAMP signaling pathway regulates heterochromatin landscape in fission yeast.https://doi.org/10.1371/journal.pgen.1010049 |
| spellingShingle | Kehan Bao Chun-Min Shan Xiao Chen Gulzhan Raiymbek Jeremy G Monroe Yimeng Fang Takenori Toda Kristin S Koutmou Kaushik Ragunathan Chao Lu Luke E Berchowitz Songtao Jia The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. PLoS Genetics |
| title | The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. |
| title_full | The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. |
| title_fullStr | The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. |
| title_full_unstemmed | The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. |
| title_short | The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly. |
| title_sort | camp signaling pathway regulates epe1 protein levels and heterochromatin assembly |
| url | https://doi.org/10.1371/journal.pgen.1010049 |
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