The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity
Nutmeg (<i>Myristica fragrans</i>) essential oil has antimicrobial, antiseptic, antiparasitic, anti-inflammatory, and antioxidant properties. We have recently demonstrated that hydrodistillation of nutmeg essential oil by applying magnesium aluminometasilicate as an excipient significant...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/9/1/37 |
| _version_ | 1818380239394308096 |
|---|---|
| author | Inga Matulyte Aiste Jekabsone Lina Jankauskaite Paulina Zavistanaviciute Vytaute Sakiene Elena Bartkiene Modestas Ruzauskas Dalia M. Kopustinskiene Antonello Santini Jurga Bernatoniene |
| author_facet | Inga Matulyte Aiste Jekabsone Lina Jankauskaite Paulina Zavistanaviciute Vytaute Sakiene Elena Bartkiene Modestas Ruzauskas Dalia M. Kopustinskiene Antonello Santini Jurga Bernatoniene |
| author_sort | Inga Matulyte |
| collection | DOAJ |
| description | Nutmeg (<i>Myristica fragrans</i>) essential oil has antimicrobial, antiseptic, antiparasitic, anti-inflammatory, and antioxidant properties. We have recently demonstrated that hydrodistillation of nutmeg essential oil by applying magnesium aluminometasilicate as an excipient significantly increases both the content and amount of bioactive substances in the oil and hydrolats. In this study, we aimed to compare the antioxidant, antimicrobial, and anti-inflammatory activity of hydrolats and essential oil obtained by hydrodistillation in the presence and absence of magnesium aluminometasilicate as an excipient. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method revealed that magnesium aluminometasilicate did not significantly improved antioxidant activity of both essential oil and hydrolat. Antibacterial efficiency was evaluated by monitoring growth of 15 bacterial strains treated by a range of dilutions of the essential oil and the hydrolats. Essential oil with an excipient completely inhibited the growth of <i>E. faecalis</i>, <i>S. mutans</i> (referent), and <i>P. multocida</i>, whereas the pure oil was only efficient against the latter strain. Finally, the anti-inflammatory properties of the substances were assessed in a fibroblast cell culture treated with viral dsRNR mimetic Poly I:C. The essential oil with an excipient protected cells against Poly I:C-induced necrosis more efficiently compared to pure essential oil. Also, both the oil and the hydrolats with aluminometasilicate were more efficient in preventing IL-6 release in the presence of Poly I:C. Our results show that the use of magnesium aluminometasilicate as an excipient might change and in some cases improve the biological activities of nutmeg essential oil and hydrolats. |
| first_indexed | 2024-12-14T02:15:31Z |
| format | Article |
| id | doaj.art-1c26b9e66807461296e78a7a928e4c61 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-14T02:15:31Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-1c26b9e66807461296e78a7a928e4c612022-12-21T23:20:38ZengMDPI AGFoods2304-81582020-01-01913710.3390/foods9010037foods9010037The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory ActivityInga Matulyte0Aiste Jekabsone1Lina Jankauskaite2Paulina Zavistanaviciute3Vytaute Sakiene4Elena Bartkiene5Modestas Ruzauskas6Dalia M. Kopustinskiene7Antonello Santini8Jurga Bernatoniene9Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Medical Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Medical Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Food Safety and Quality, Lithuanian University of Health Sciences, LT-47181 Kaunas, LithuaniaDepartment of Food Safety and Quality, Lithuanian University of Health Sciences, LT-47181 Kaunas, LithuaniaDepartment of Food Safety and Quality, Lithuanian University of Health Sciences, LT-47181 Kaunas, LithuaniaInstitute of Microbiology and Virology, Lithuanian University of Health Sciences, LT-47181 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Medical Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Napoli, ItalyDepartment of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaNutmeg (<i>Myristica fragrans</i>) essential oil has antimicrobial, antiseptic, antiparasitic, anti-inflammatory, and antioxidant properties. We have recently demonstrated that hydrodistillation of nutmeg essential oil by applying magnesium aluminometasilicate as an excipient significantly increases both the content and amount of bioactive substances in the oil and hydrolats. In this study, we aimed to compare the antioxidant, antimicrobial, and anti-inflammatory activity of hydrolats and essential oil obtained by hydrodistillation in the presence and absence of magnesium aluminometasilicate as an excipient. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method revealed that magnesium aluminometasilicate did not significantly improved antioxidant activity of both essential oil and hydrolat. Antibacterial efficiency was evaluated by monitoring growth of 15 bacterial strains treated by a range of dilutions of the essential oil and the hydrolats. Essential oil with an excipient completely inhibited the growth of <i>E. faecalis</i>, <i>S. mutans</i> (referent), and <i>P. multocida</i>, whereas the pure oil was only efficient against the latter strain. Finally, the anti-inflammatory properties of the substances were assessed in a fibroblast cell culture treated with viral dsRNR mimetic Poly I:C. The essential oil with an excipient protected cells against Poly I:C-induced necrosis more efficiently compared to pure essential oil. Also, both the oil and the hydrolats with aluminometasilicate were more efficient in preventing IL-6 release in the presence of Poly I:C. Our results show that the use of magnesium aluminometasilicate as an excipient might change and in some cases improve the biological activities of nutmeg essential oil and hydrolats.https://www.mdpi.com/2304-8158/9/1/37nutmegessential oilantioxidant activityantibacterial activitypoly i:c-induced inflammationfibroblastsmagnesium aluminometasilicate |
| spellingShingle | Inga Matulyte Aiste Jekabsone Lina Jankauskaite Paulina Zavistanaviciute Vytaute Sakiene Elena Bartkiene Modestas Ruzauskas Dalia M. Kopustinskiene Antonello Santini Jurga Bernatoniene The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity Foods nutmeg essential oil antioxidant activity antibacterial activity poly i:c-induced inflammation fibroblasts magnesium aluminometasilicate |
| title | The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity |
| title_full | The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity |
| title_fullStr | The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity |
| title_full_unstemmed | The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity |
| title_short | The Essential Oil and Hydrolats from <i>Myristica fragrans</i> Seeds with Magnesium Aluminometasilicate as Excipient: Antioxidant, Antibacterial, and Anti-inflammatory Activity |
| title_sort | essential oil and hydrolats from i myristica fragrans i seeds with magnesium aluminometasilicate as excipient antioxidant antibacterial and anti inflammatory activity |
| topic | nutmeg essential oil antioxidant activity antibacterial activity poly i:c-induced inflammation fibroblasts magnesium aluminometasilicate |
| url | https://www.mdpi.com/2304-8158/9/1/37 |
| work_keys_str_mv | AT ingamatulyte theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT aistejekabsone theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT linajankauskaite theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT paulinazavistanaviciute theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT vytautesakiene theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT elenabartkiene theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT modestasruzauskas theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT daliamkopustinskiene theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT antonellosantini theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT jurgabernatoniene theessentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT ingamatulyte essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT aistejekabsone essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT linajankauskaite essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT paulinazavistanaviciute essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT vytautesakiene essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT elenabartkiene essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT modestasruzauskas essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT daliamkopustinskiene essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT antonellosantini essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity AT jurgabernatoniene essentialoilandhydrolatsfromimyristicafragransiseedswithmagnesiumaluminometasilicateasexcipientantioxidantantibacterialandantiinflammatoryactivity |