Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer
Abstract Cervical cancer is the fourth highest mortality cancer among women worldwide. Many researchers have discovered the major anticancer role of miR‐192‐5p. However, no study has revealed the effect of miR‐192‐5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | https://doi.org/10.1002/kjm2.12398 |
| _version_ | 1818681884095283200 |
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| author | Ruo‐Fan Dong Yong‐Ju Zhuang Yuan Wang Zhen‐Yu Zhang Xi‐Zhong Xu Yu‐Rong Mao Jin‐Jin Yu |
| author_facet | Ruo‐Fan Dong Yong‐Ju Zhuang Yuan Wang Zhen‐Yu Zhang Xi‐Zhong Xu Yu‐Rong Mao Jin‐Jin Yu |
| author_sort | Ruo‐Fan Dong |
| collection | DOAJ |
| description | Abstract Cervical cancer is the fourth highest mortality cancer among women worldwide. Many researchers have discovered the major anticancer role of miR‐192‐5p. However, no study has revealed the effect of miR‐192‐5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed to explore the role of miR‐192‐5p in proliferation, invasion of cervical cancer, and its regulatory mechanism. Firstly, the expression level of miR‐192‐5p was examined by real‐time quantitative polymerase chain reaction. Cell counting kit‐8 analysis was applied to detect the proliferation of transfected Caski and SiHa cells. Flow cytometry assay was applied to detect the apoptosis of transfected Caski and SiHa cells. Our result showed that miR‐192‐5p restrained cervical cancer cell proliferation and induced apoptosis. Then we employed wound healing and transwell assays to analyze the migration and invasion abilities of Caski and SiHa cells in vitro. The results showed that miR‐192‐5p had an inhibitory effect on cervical cancer migration and invasion. The results of in vivo experiment demonstrated that miR‐192‐5p also inhibited tumor development in nude mice. We further detected that the binding of transient receptor potential melastatin‐subfamily member 7 (TRPM7) to miR‐192‐5p using bioinformatic methods and dual‐luciferase reporter assay. Finally, we found that TRPM7 overexpression reversed the inhibitory effects of miR‐192‐5p on proliferation, migration, and invasion on cervical cancer cells. In conclusion, the findings of the present study revealed that miR‐192‐5p performs an inhibitory role in cervical cancer proliferation and invasion by targeting TRPM7. |
| first_indexed | 2024-12-17T10:10:02Z |
| format | Article |
| id | doaj.art-1c283678b10549a59e0d135a956727b4 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-17T10:10:02Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-1c283678b10549a59e0d135a956727b42022-12-21T21:53:02ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-08-0137869970810.1002/kjm2.12398Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancerRuo‐Fan Dong0Yong‐Ju Zhuang1Yuan Wang2Zhen‐Yu Zhang3Xi‐Zhong Xu4Yu‐Rong Mao5Jin‐Jin Yu6Department of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaDepartment of Gynecology Affiliated Hospital of Jiangnan University Wuxi ChinaAbstract Cervical cancer is the fourth highest mortality cancer among women worldwide. Many researchers have discovered the major anticancer role of miR‐192‐5p. However, no study has revealed the effect of miR‐192‐5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed to explore the role of miR‐192‐5p in proliferation, invasion of cervical cancer, and its regulatory mechanism. Firstly, the expression level of miR‐192‐5p was examined by real‐time quantitative polymerase chain reaction. Cell counting kit‐8 analysis was applied to detect the proliferation of transfected Caski and SiHa cells. Flow cytometry assay was applied to detect the apoptosis of transfected Caski and SiHa cells. Our result showed that miR‐192‐5p restrained cervical cancer cell proliferation and induced apoptosis. Then we employed wound healing and transwell assays to analyze the migration and invasion abilities of Caski and SiHa cells in vitro. The results showed that miR‐192‐5p had an inhibitory effect on cervical cancer migration and invasion. The results of in vivo experiment demonstrated that miR‐192‐5p also inhibited tumor development in nude mice. We further detected that the binding of transient receptor potential melastatin‐subfamily member 7 (TRPM7) to miR‐192‐5p using bioinformatic methods and dual‐luciferase reporter assay. Finally, we found that TRPM7 overexpression reversed the inhibitory effects of miR‐192‐5p on proliferation, migration, and invasion on cervical cancer cells. In conclusion, the findings of the present study revealed that miR‐192‐5p performs an inhibitory role in cervical cancer proliferation and invasion by targeting TRPM7.https://doi.org/10.1002/kjm2.12398cervical cancerinvasionmiR‐192‐5pproliferationTRPM7 |
| spellingShingle | Ruo‐Fan Dong Yong‐Ju Zhuang Yuan Wang Zhen‐Yu Zhang Xi‐Zhong Xu Yu‐Rong Mao Jin‐Jin Yu Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer Kaohsiung Journal of Medical Sciences cervical cancer invasion miR‐192‐5p proliferation TRPM7 |
| title | Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer |
| title_full | Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer |
| title_fullStr | Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer |
| title_full_unstemmed | Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer |
| title_short | Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer |
| title_sort | tumor suppressor mir 192 5p targets trpm7 and inhibits proliferation and invasion in cervical cancer |
| topic | cervical cancer invasion miR‐192‐5p proliferation TRPM7 |
| url | https://doi.org/10.1002/kjm2.12398 |
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