Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities
The root of <i>Chuanminshen violaceum</i> is used as an important edible and medicinal plant in China. However, its leaves are generally considered byproducts, and therefore do not have a use. Thus, the phenolic compounds in the methanolic extracts (CVLMs) and the chemical characteristic...
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MDPI AG
2019-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/8/266 |
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| author | Shang Lin Hong-Yi Li Zi-Ying Wang Xin Liu Yang Yang Zheng-Wen Cao Gang Du Li Zhao Qing Zhang Ding-Tao Wu Wen Qin |
| author_facet | Shang Lin Hong-Yi Li Zi-Ying Wang Xin Liu Yang Yang Zheng-Wen Cao Gang Du Li Zhao Qing Zhang Ding-Tao Wu Wen Qin |
| author_sort | Shang Lin |
| collection | DOAJ |
| description | The root of <i>Chuanminshen violaceum</i> is used as an important edible and medicinal plant in China. However, its leaves are generally considered byproducts, and therefore do not have a use. Thus, the phenolic compounds in the methanolic extracts (CVLMs) and the chemical characteristics of crude polysaccharides (CVLPs) from the leaves of <i>C. violaceum</i> and their in vitro antioxidant activities were explored. The results showed that chlorogenic acid and rutin were the major individual phenolic compounds in the leaves, which ranged from 1.22 ± 0.03 to 2.87 ± 0.04 mg/g DW, and from 2.25 ± 0.04 to 4.03 ± 0.05 mg/g DW, respectively. Meanwhile, the extraction yields of CVLPs from the leaves ranged from 4.73% to 5.41%. The CVLPs consisted of mannose, rhamnose, galacturonic acid, glucose, galactose, and arabinose, suggesting the existence of pectic polysaccharides. Furthermore, both CVLMs and CVLPs exhibited strong antioxidant activities. Chlorogenic acid and rutin were major contributors to the antioxidant activities of CVLMs, and the antioxidant activities of CVLPs were closely correlated to their α-1,4-D-galactosiduronic linkages. The results are beneficial for understanding the chemical properties and in vitro antioxidant activities of CVLMs and CVLPs. The leaves of <i>C. violaceum</i> have potential to be developed as natural antioxidants. |
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| issn | 2076-3921 |
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| last_indexed | 2024-03-12T20:09:23Z |
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| series | Antioxidants |
| spelling | doaj.art-1c2b60f331d94850b1248d832a2e56952023-08-02T01:47:17ZengMDPI AGAntioxidants2076-39212019-08-018826610.3390/antiox8080266antiox8080266Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant ActivitiesShang Lin0Hong-Yi Li1Zi-Ying Wang2Xin Liu3Yang Yang4Zheng-Wen Cao5Gang Du6Li Zhao7Qing Zhang8Ding-Tao Wu9Wen Qin10Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaSichuan Provincial Institute for Food and Drug Control, Chengdu 611730, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaThe root of <i>Chuanminshen violaceum</i> is used as an important edible and medicinal plant in China. However, its leaves are generally considered byproducts, and therefore do not have a use. Thus, the phenolic compounds in the methanolic extracts (CVLMs) and the chemical characteristics of crude polysaccharides (CVLPs) from the leaves of <i>C. violaceum</i> and their in vitro antioxidant activities were explored. The results showed that chlorogenic acid and rutin were the major individual phenolic compounds in the leaves, which ranged from 1.22 ± 0.03 to 2.87 ± 0.04 mg/g DW, and from 2.25 ± 0.04 to 4.03 ± 0.05 mg/g DW, respectively. Meanwhile, the extraction yields of CVLPs from the leaves ranged from 4.73% to 5.41%. The CVLPs consisted of mannose, rhamnose, galacturonic acid, glucose, galactose, and arabinose, suggesting the existence of pectic polysaccharides. Furthermore, both CVLMs and CVLPs exhibited strong antioxidant activities. Chlorogenic acid and rutin were major contributors to the antioxidant activities of CVLMs, and the antioxidant activities of CVLPs were closely correlated to their α-1,4-D-galactosiduronic linkages. The results are beneficial for understanding the chemical properties and in vitro antioxidant activities of CVLMs and CVLPs. The leaves of <i>C. violaceum</i> have potential to be developed as natural antioxidants.https://www.mdpi.com/2076-3921/8/8/266<i>Chuanminshen violaceum</i>phenolic compoundpolysaccharidechemical structureantioxidant activity |
| spellingShingle | Shang Lin Hong-Yi Li Zi-Ying Wang Xin Liu Yang Yang Zheng-Wen Cao Gang Du Li Zhao Qing Zhang Ding-Tao Wu Wen Qin Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities Antioxidants <i>Chuanminshen violaceum</i> phenolic compound polysaccharide chemical structure antioxidant activity |
| title | Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities |
| title_full | Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities |
| title_fullStr | Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities |
| title_full_unstemmed | Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities |
| title_short | Analysis of Methanolic Extracts and Crude Polysaccharides from the Leaves of <i>Chuanminshen violaceum</i> and Their Antioxidant Activities |
| title_sort | analysis of methanolic extracts and crude polysaccharides from the leaves of i chuanminshen violaceum i and their antioxidant activities |
| topic | <i>Chuanminshen violaceum</i> phenolic compound polysaccharide chemical structure antioxidant activity |
| url | https://www.mdpi.com/2076-3921/8/8/266 |
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