Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy
BackgroundGastrectomy is the most effective treatment to improve the clinical survival rate of patients with gastric cancer. However, the pathophysiological changes caused by gastrectomy have seriously affected the postoperative recovery.MethodsIn the present trial, Ataining (containing C. butyricum...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1076245/full |
| _version_ | 1797983669683486720 |
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| author | Wenjie Cao Cihua Zheng Xuan Xu Rui Jin Feng Huang Meng Shi Zhipeng He Yufeng Luo Lulin Liu Zhaoxia Liu Jing Wei Xiaorong Deng Tingtao Chen Tingtao Chen |
| author_facet | Wenjie Cao Cihua Zheng Xuan Xu Rui Jin Feng Huang Meng Shi Zhipeng He Yufeng Luo Lulin Liu Zhaoxia Liu Jing Wei Xiaorong Deng Tingtao Chen Tingtao Chen |
| author_sort | Wenjie Cao |
| collection | DOAJ |
| description | BackgroundGastrectomy is the most effective treatment to improve the clinical survival rate of patients with gastric cancer. However, the pathophysiological changes caused by gastrectomy have seriously affected the postoperative recovery.MethodsIn the present trial, Ataining (containing C. butyricum, CGMCC0313.1) was applied in patients after gastrectomy to investigate the effect of C. butyricum on the early postoperative recovery by monitoring the inflammatory immune response with blood indicators, detecting the gut microbiota with high-throughput sequencing, and analyzing the short-chain fatty acids (SCFAs) with targeted metabolomics. This study is registered with the number ChiCTR2000040915.ResultsOur outcomes revealed that C. butyricum had significantly reduced the number of Leucocyte (P < 0.001), the percentage of Neutrophil (P < 0.001), the expression of IL-1β (P < 0.01), IL-6 (P < 0.05), and TNF-α (P < 0.01), while markedly enhanced the immunity indexes (immunoglobulin and lymphocyte) (P < 0.05) and nutrition indexes (albumin and total protein) (P < 0.05). In addition, the use of the C. butyricum greatly enriched the relative abundance of beneficial bacteria Bacteroides, Faecalibacterium and Gemmiger, while the abundance of pathogenic Streptococcus, Desulfovibrio and Actinomyces were markedly decreased at genus level. We also observed significant up-regulation of SCFAs, including acetic acid, propionic acid, butyric acid and isobutyric acid, after C. butyricum administration in patients receiving gastrectomy.ConclusionTherefore, evidence supported that oral administration of C. butyricum after gastrectomy can reduce early postoperative inflammation, enhance immune ability, restore intestinal microbiota eubiosis, increase intestinal SCFAs, reduce the occurrence of postoperative complications, and ultimately promote the early recovery of the patient.Clinical trial registrationhttp://www.chictr.org.cn/, identifier (ChiCTR2000040915). |
| first_indexed | 2024-04-11T06:50:55Z |
| format | Article |
| id | doaj.art-1c2b7d5b891a468a9e9d002bde7bdb8b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T06:50:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-1c2b7d5b891a468a9e9d002bde7bdb8b2022-12-22T04:39:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.10762451076245Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomyWenjie Cao0Cihua Zheng1Xuan Xu2Rui Jin3Feng Huang4Meng Shi5Zhipeng He6Yufeng Luo7Lulin Liu8Zhaoxia Liu9Jing Wei10Xiaorong Deng11Tingtao Chen12Tingtao Chen13Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaHuanKui Academy, Nanchang University, Nanchang, ChinaQueen Mary College, Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaNational Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, ChinaBackgroundGastrectomy is the most effective treatment to improve the clinical survival rate of patients with gastric cancer. However, the pathophysiological changes caused by gastrectomy have seriously affected the postoperative recovery.MethodsIn the present trial, Ataining (containing C. butyricum, CGMCC0313.1) was applied in patients after gastrectomy to investigate the effect of C. butyricum on the early postoperative recovery by monitoring the inflammatory immune response with blood indicators, detecting the gut microbiota with high-throughput sequencing, and analyzing the short-chain fatty acids (SCFAs) with targeted metabolomics. This study is registered with the number ChiCTR2000040915.ResultsOur outcomes revealed that C. butyricum had significantly reduced the number of Leucocyte (P < 0.001), the percentage of Neutrophil (P < 0.001), the expression of IL-1β (P < 0.01), IL-6 (P < 0.05), and TNF-α (P < 0.01), while markedly enhanced the immunity indexes (immunoglobulin and lymphocyte) (P < 0.05) and nutrition indexes (albumin and total protein) (P < 0.05). In addition, the use of the C. butyricum greatly enriched the relative abundance of beneficial bacteria Bacteroides, Faecalibacterium and Gemmiger, while the abundance of pathogenic Streptococcus, Desulfovibrio and Actinomyces were markedly decreased at genus level. We also observed significant up-regulation of SCFAs, including acetic acid, propionic acid, butyric acid and isobutyric acid, after C. butyricum administration in patients receiving gastrectomy.ConclusionTherefore, evidence supported that oral administration of C. butyricum after gastrectomy can reduce early postoperative inflammation, enhance immune ability, restore intestinal microbiota eubiosis, increase intestinal SCFAs, reduce the occurrence of postoperative complications, and ultimately promote the early recovery of the patient.Clinical trial registrationhttp://www.chictr.org.cn/, identifier (ChiCTR2000040915).https://www.frontiersin.org/articles/10.3389/fimmu.2022.1076245/fullGastric cancerClostridium butyricumSCFAsgut microbiotagastrectomy |
| spellingShingle | Wenjie Cao Cihua Zheng Xuan Xu Rui Jin Feng Huang Meng Shi Zhipeng He Yufeng Luo Lulin Liu Zhaoxia Liu Jing Wei Xiaorong Deng Tingtao Chen Tingtao Chen Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy Frontiers in Immunology Gastric cancer Clostridium butyricum SCFAs gut microbiota gastrectomy |
| title | Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| title_full | Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| title_fullStr | Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| title_full_unstemmed | Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| title_short | Clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| title_sort | clostridium butyricum potentially improves inflammation and immunity through alteration of the microbiota and metabolism of gastric cancer patients after gastrectomy |
| topic | Gastric cancer Clostridium butyricum SCFAs gut microbiota gastrectomy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1076245/full |
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