Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyph...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020317916 |
| _version_ | 1818514390871179264 |
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| author | Neeragunda Shivaraj Yathisha Plancot Barbara Bruno Gügi Kambalagere Yogendra Sudisha Jogaiah Driouich Azeddine Ramasandra Govind Sharatchandra |
| author_facet | Neeragunda Shivaraj Yathisha Plancot Barbara Bruno Gügi Kambalagere Yogendra Sudisha Jogaiah Driouich Azeddine Ramasandra Govind Sharatchandra |
| author_sort | Neeragunda Shivaraj Yathisha |
| collection | DOAJ |
| description | Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyphylla by evaluating its ability to recover the physiological, biochemical and morphological functions post desiccation. In order to understand the responses of Eragrostiella brachyphylla to desiccation and subsequent rehydration experiments were conducted in the hydrated state (HS), desiccated state (DS) and rehydrated state (RS). Scanning electron microscopy revealed significant changes between the three stages in the internal ultra-structures of leaves and stems. Compared to the other states, photosynthetic parameters such as chlorophyll a, chlorophyll b, total chlorophylland total carotenoid contents decreased significantly in the desiccated state. Superoxide radical (O2•−) content also increased, resulting in an oxidative burst during desiccation. Consequently, antioxidant enzymes such as catalase (CAT) superoxide dismutase (SOD) peroxidase (APX) and glutathione reductase (GR) activities were found to be significantly elevated in the desiccated state to avoid oxidative damage. Increased malondialdehyde (MDA) content and relative electrolyte leakage (REL) during desiccation provide evidence for membrane damage and loss of cell-wall integrity. During desiccation, the contents of osmolytes represented by sucrose and proline were found to increase to maintain cell structure integrity. After rehydration, all physiological, biochemical and morphological properties remain unchanged or slightly changed when compared to the hydrated state. Hence, we believe that these unique adaptations contribute to the remarkable desiccation-tolerance property of this plant. |
| first_indexed | 2024-12-11T00:15:05Z |
| format | Article |
| id | doaj.art-1c2def5eed474d32add579f367e9d57f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T00:15:05Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1c2def5eed474d32add579f367e9d57f2022-12-22T01:27:58ZengElsevierHeliyon2405-84402020-09-0169e04948Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responsesNeeragunda Shivaraj Yathisha0Plancot Barbara1Bruno Gügi2Kambalagere Yogendra3Sudisha Jogaiah4Driouich Azeddine5Ramasandra Govind Sharatchandra6Department of Studies and Research in Biotechnology and Microbiology, Tumkur University, Tumakuru, 57210, India; Department of Studies and Research in Environmental Science, Kuvempu University, Shankaraghatta, Shimoga, 577451, IndiaLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale, Normandie University, University of Rouen, 76000, Rouen, France; Fédération de Recherche “Normandie-Végétal”-FED 4277, 76000, Rouen, FranceLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale, Normandie University, University of Rouen, 76000, Rouen, France; Fédération de Recherche “Normandie-Végétal”-FED 4277, 76000, Rouen, FranceDepartment of Studies and Research in Environmental Science, Kuvempu University, Shankaraghatta, Shimoga, 577451, IndiaDepartment of Studies and Research in Biotechnology and Microbiology, Karnataka University, Dharwad, IndiaLaboratoire de Glycobiologie et Matrice Extracellulaire Végétale, Normandie University, University of Rouen, 76000, Rouen, France; Fédération de Recherche “Normandie-Végétal”-FED 4277, 76000, Rouen, FranceDepartment of Studies and Research in Biotechnology and Microbiology, Tumkur University, Tumakuru, 57210, India; Corresponding author.Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyphylla by evaluating its ability to recover the physiological, biochemical and morphological functions post desiccation. In order to understand the responses of Eragrostiella brachyphylla to desiccation and subsequent rehydration experiments were conducted in the hydrated state (HS), desiccated state (DS) and rehydrated state (RS). Scanning electron microscopy revealed significant changes between the three stages in the internal ultra-structures of leaves and stems. Compared to the other states, photosynthetic parameters such as chlorophyll a, chlorophyll b, total chlorophylland total carotenoid contents decreased significantly in the desiccated state. Superoxide radical (O2•−) content also increased, resulting in an oxidative burst during desiccation. Consequently, antioxidant enzymes such as catalase (CAT) superoxide dismutase (SOD) peroxidase (APX) and glutathione reductase (GR) activities were found to be significantly elevated in the desiccated state to avoid oxidative damage. Increased malondialdehyde (MDA) content and relative electrolyte leakage (REL) during desiccation provide evidence for membrane damage and loss of cell-wall integrity. During desiccation, the contents of osmolytes represented by sucrose and proline were found to increase to maintain cell structure integrity. After rehydration, all physiological, biochemical and morphological properties remain unchanged or slightly changed when compared to the hydrated state. Hence, we believe that these unique adaptations contribute to the remarkable desiccation-tolerance property of this plant.http://www.sciencedirect.com/science/article/pii/S2405844020317916Plant biologyDesiccation toleranceRWCUltrastructurePhotosynthesisAntioxidant enzymes |
| spellingShingle | Neeragunda Shivaraj Yathisha Plancot Barbara Bruno Gügi Kambalagere Yogendra Sudisha Jogaiah Driouich Azeddine Ramasandra Govind Sharatchandra Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses Heliyon Plant biology Desiccation tolerance RWC Ultrastructure Photosynthesis Antioxidant enzymes |
| title | Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses |
| title_full | Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses |
| title_fullStr | Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses |
| title_full_unstemmed | Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses |
| title_short | Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses |
| title_sort | vegetative desiccation tolerance in eragrostiella brachyphylla biochemical and physiological responses |
| topic | Plant biology Desiccation tolerance RWC Ultrastructure Photosynthesis Antioxidant enzymes |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020317916 |
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