Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture
Abstract Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-02-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.972 |
| _version_ | 1818304658819514368 |
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| author | Lázaro López Diana Calderón Paúl Cardenas María B. Prado Carlos Valle Gabriel Trueba |
| author_facet | Lázaro López Diana Calderón Paúl Cardenas María B. Prado Carlos Valle Gabriel Trueba |
| author_sort | Lázaro López |
| collection | DOAJ |
| description | Abstract Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they lose some traits required to thrive in the original niche. In this study, a strain of Lactobacillus reuteri was isolated from mouse duodenum and subjected to 150 serial passes in milk to simulate the industrial propagation of probiotic bacteria. The strains adapted to milk outperformed their ancestor when grown in milk; we also showed evidence of reduced intestinal colonization of milk‐adapted strains. Whole‐genome sequencing showed that bacterial adaptation to milk selects mutants with altered metabolic functions. |
| first_indexed | 2024-12-13T06:14:12Z |
| format | Article |
| id | doaj.art-1c2e39e3bced44daa2b332442d651a93 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-13T06:14:12Z |
| publishDate | 2020-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-1c2e39e3bced44daa2b332442d651a932022-12-21T23:57:00ZengWileyMicrobiologyOpen2045-88272020-02-0192n/an/a10.1002/mbo3.972Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufactureLázaro López0Diana Calderón1Paúl Cardenas2María B. Prado3Carlos Valle4Gabriel Trueba5Instituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorInstituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorInstituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorInstituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorInstituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorInstituto de Microbiología Colegio de Ciencias Biológicas y Ambientales Universidad San Francisco de Quito Quito EcuadorAbstract Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they lose some traits required to thrive in the original niche. In this study, a strain of Lactobacillus reuteri was isolated from mouse duodenum and subjected to 150 serial passes in milk to simulate the industrial propagation of probiotic bacteria. The strains adapted to milk outperformed their ancestor when grown in milk; we also showed evidence of reduced intestinal colonization of milk‐adapted strains. Whole‐genome sequencing showed that bacterial adaptation to milk selects mutants with altered metabolic functions.https://doi.org/10.1002/mbo3.972antagonistic pleiotropyexperimental evolutionLactobacillus reuteriprobiotics |
| spellingShingle | Lázaro López Diana Calderón Paúl Cardenas María B. Prado Carlos Valle Gabriel Trueba Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture MicrobiologyOpen antagonistic pleiotropy experimental evolution Lactobacillus reuteri probiotics |
| title | Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture |
| title_full | Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture |
| title_fullStr | Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture |
| title_full_unstemmed | Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture |
| title_short | Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture |
| title_sort | evolutionary changes of an intestinal lactobacillus reuteri during probiotic manufacture |
| topic | antagonistic pleiotropy experimental evolution Lactobacillus reuteri probiotics |
| url | https://doi.org/10.1002/mbo3.972 |
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