Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran
Background: is an increasingly important cause of nosocomial infections worldwide. In addition to the intrinsic resistance of to many antibiotics, available treatment approaches with older antibiotics are significantly associated with an increase in multiresistant strains. The aim of this study wa...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2019-08-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000324.shtml |
| _version_ | 1811339865944489984 |
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| author | Valadan Tahbaz, Sahel Azimi, Leila Asadian, Mahla Lari, Abdolaziz Rastegar |
| author_facet | Valadan Tahbaz, Sahel Azimi, Leila Asadian, Mahla Lari, Abdolaziz Rastegar |
| author_sort | Valadan Tahbaz, Sahel |
| collection | DOAJ |
| description | Background: is an increasingly important cause of nosocomial infections worldwide. In addition to the intrinsic resistance of to many antibiotics, available treatment approaches with older antibiotics are significantly associated with an increase in multiresistant strains. The aim of this study was to evaluate the synergistic effect of tazobactam with meropenem and ciprofloxacin against carbapenems and drug resistant isolated from burn patients in a tertiary burn center in Tehran.Materials and methods: In this study, a total of 47 clinical isolates of were included from burn patients admitted to the Shahid Motahari Burns Hospital, Tehran, from June 2018 to August 2018. The disk diffusion method was used to determine resistance patterns. The synergistic effect of tazobactam with meropenem and ciprofloxacin was evaluated by determining the MIC. A PCR assay was performed to determine , and .Results: Antibiotic susceptibility testing revealed that all of the isolates were resistant to meropenem and ciprofloxacin. The MIC values decreased in the cases of combined use of ciprofloxacin and meropenem with tazobactam. The bla gene was the predominant carbapenemase gene (93.6%), followed by , which was detected in 48.9% of isolates. None of the isolates harbored the gene. Conclusions: Based on in-vitro antimicrobial susceptibility in the current study, the MIC of tazobactam combined with meropenem or ciprofloxacin have been shown to be variable. Furthermore, the data acquired from such conditions should be confirmed by reliable results from sufficiently controlled clinical trials. |
| first_indexed | 2024-04-13T18:32:56Z |
| format | Article |
| id | doaj.art-1c310f56ec5441078273ffd170b845cd |
| institution | Directory Open Access Journal |
| issn | 2196-5226 |
| language | deu |
| last_indexed | 2024-04-13T18:32:56Z |
| publishDate | 2019-08-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj.art-1c310f56ec5441078273ffd170b845cd2022-12-22T02:34:59ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262019-08-0114Doc0810.3205/dgkh000324Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in TehranValadan Tahbaz, Sahel0Azimi, Leila1Asadian, Mahla2Lari, Abdolaziz Rastegar3Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, IranPediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, IranDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Iran University of Medical Sciences, Tehran, IranBackground: is an increasingly important cause of nosocomial infections worldwide. In addition to the intrinsic resistance of to many antibiotics, available treatment approaches with older antibiotics are significantly associated with an increase in multiresistant strains. The aim of this study was to evaluate the synergistic effect of tazobactam with meropenem and ciprofloxacin against carbapenems and drug resistant isolated from burn patients in a tertiary burn center in Tehran.Materials and methods: In this study, a total of 47 clinical isolates of were included from burn patients admitted to the Shahid Motahari Burns Hospital, Tehran, from June 2018 to August 2018. The disk diffusion method was used to determine resistance patterns. The synergistic effect of tazobactam with meropenem and ciprofloxacin was evaluated by determining the MIC. A PCR assay was performed to determine , and .Results: Antibiotic susceptibility testing revealed that all of the isolates were resistant to meropenem and ciprofloxacin. The MIC values decreased in the cases of combined use of ciprofloxacin and meropenem with tazobactam. The bla gene was the predominant carbapenemase gene (93.6%), followed by , which was detected in 48.9% of isolates. None of the isolates harbored the gene. Conclusions: Based on in-vitro antimicrobial susceptibility in the current study, the MIC of tazobactam combined with meropenem or ciprofloxacin have been shown to be variable. Furthermore, the data acquired from such conditions should be confirmed by reliable results from sufficiently controlled clinical trials.http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000324.shtmlAcinetobacter baumanniitazobactamMICcarbapenem resistance |
| spellingShingle | Valadan Tahbaz, Sahel Azimi, Leila Asadian, Mahla Lari, Abdolaziz Rastegar Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran GMS Hygiene and Infection Control Acinetobacter baumannii tazobactam MIC carbapenem resistance |
| title | Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran |
| title_full | Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran |
| title_fullStr | Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran |
| title_full_unstemmed | Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran |
| title_short | Evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi-drug resistant Acinetobacter baumannii isolated from burn patients in Tehran |
| title_sort | evaluation of synergistic effect of tazobactam with meropenem and ciprofloxacin against multi drug resistant acinetobacter baumannii isolated from burn patients in tehran |
| topic | Acinetobacter baumannii tazobactam MIC carbapenem resistance |
| url | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000324.shtml |
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