Mating disrupts morning anticipation in Drosophila melanogaster females.
After mating, the physiology of Drosophila females undergo several important changes, some of which are reflected in their rest-activity cycles. To explore the hypothesis that mating modifies the temporal organization of locomotor activity patterns, we recorded fly activity by a video tracking metho...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-12-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010258 |
| _version_ | 1797782601247752192 |
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| author | Sabrina Riva Juan Ignacio Ispizua María Trinidad Breide Sofía Polcowñuk José Ricardo Lobera María Fernanda Ceriani Sebastian Risau-Gusman Diana Lorena Franco |
| author_facet | Sabrina Riva Juan Ignacio Ispizua María Trinidad Breide Sofía Polcowñuk José Ricardo Lobera María Fernanda Ceriani Sebastian Risau-Gusman Diana Lorena Franco |
| author_sort | Sabrina Riva |
| collection | DOAJ |
| description | After mating, the physiology of Drosophila females undergo several important changes, some of which are reflected in their rest-activity cycles. To explore the hypothesis that mating modifies the temporal organization of locomotor activity patterns, we recorded fly activity by a video tracking method. Monitoring rest-activity patterns under light/dark (LD) cycles indicated that mated females lose their ability to anticipate the night-day transition, in stark contrast to males and virgins. This postmating response is mediated by the activation of the sex peptide receptor (SPR) mainly on pickpocket (ppk) expressing neurons, since reducing expression of this receptor in these neurons restores the ability to anticipate the LD transition in mated females. Furthermore, we provide evidence of connectivity between ppk+ neurons and the pigment-dispersing factor (PDF)-positive ventral lateral neurons (sLNv), which play a central role in the temporal organization of daily activity. Since PDF has been associated to the generation of the morning activity peak, we hypothesized that the mating signal could modulate PDF levels. Indeed, we confirm that mated females have reduced PDF levels at the dorsal protocerebrum; moreover, SPR downregulation in ppk+ neurons mimics PDF levels observed in males. In sum, our results are consistent with a model whereby mating-triggered signals reach clock neurons in the fly central nervous system to modulate the temporal organization of circadian behavior according to the needs of the new status. |
| first_indexed | 2024-03-13T00:13:15Z |
| format | Article |
| id | doaj.art-1c335177397246b6a0ac6bea3b33b393 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-03-13T00:13:15Z |
| publishDate | 2022-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-1c335177397246b6a0ac6bea3b33b3932023-07-12T05:31:40ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-12-011812e101025810.1371/journal.pgen.1010258Mating disrupts morning anticipation in Drosophila melanogaster females.Sabrina RivaJuan Ignacio IspizuaMaría Trinidad BreideSofía PolcowñukJosé Ricardo LoberaMaría Fernanda CerianiSebastian Risau-GusmanDiana Lorena FrancoAfter mating, the physiology of Drosophila females undergo several important changes, some of which are reflected in their rest-activity cycles. To explore the hypothesis that mating modifies the temporal organization of locomotor activity patterns, we recorded fly activity by a video tracking method. Monitoring rest-activity patterns under light/dark (LD) cycles indicated that mated females lose their ability to anticipate the night-day transition, in stark contrast to males and virgins. This postmating response is mediated by the activation of the sex peptide receptor (SPR) mainly on pickpocket (ppk) expressing neurons, since reducing expression of this receptor in these neurons restores the ability to anticipate the LD transition in mated females. Furthermore, we provide evidence of connectivity between ppk+ neurons and the pigment-dispersing factor (PDF)-positive ventral lateral neurons (sLNv), which play a central role in the temporal organization of daily activity. Since PDF has been associated to the generation of the morning activity peak, we hypothesized that the mating signal could modulate PDF levels. Indeed, we confirm that mated females have reduced PDF levels at the dorsal protocerebrum; moreover, SPR downregulation in ppk+ neurons mimics PDF levels observed in males. In sum, our results are consistent with a model whereby mating-triggered signals reach clock neurons in the fly central nervous system to modulate the temporal organization of circadian behavior according to the needs of the new status.https://doi.org/10.1371/journal.pgen.1010258 |
| spellingShingle | Sabrina Riva Juan Ignacio Ispizua María Trinidad Breide Sofía Polcowñuk José Ricardo Lobera María Fernanda Ceriani Sebastian Risau-Gusman Diana Lorena Franco Mating disrupts morning anticipation in Drosophila melanogaster females. PLoS Genetics |
| title | Mating disrupts morning anticipation in Drosophila melanogaster females. |
| title_full | Mating disrupts morning anticipation in Drosophila melanogaster females. |
| title_fullStr | Mating disrupts morning anticipation in Drosophila melanogaster females. |
| title_full_unstemmed | Mating disrupts morning anticipation in Drosophila melanogaster females. |
| title_short | Mating disrupts morning anticipation in Drosophila melanogaster females. |
| title_sort | mating disrupts morning anticipation in drosophila melanogaster females |
| url | https://doi.org/10.1371/journal.pgen.1010258 |
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