Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>

Proanthocyanidins are natural glycosidase inhibitors with excellent antioxidant activity. This study aims to search for a new source of proanthocyanidins for the prevention and treatment of type 2 diabetes with higher content and better activity and get their structure elucidated. First, the total p...

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Main Authors: Xin Li, Jingling Liu, Qinxiang Chang, Ziyun Zhou, Ruilian Han, Zongsuo Liang
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/9/2417
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author Xin Li
Jingling Liu
Qinxiang Chang
Ziyun Zhou
Ruilian Han
Zongsuo Liang
author_facet Xin Li
Jingling Liu
Qinxiang Chang
Ziyun Zhou
Ruilian Han
Zongsuo Liang
author_sort Xin Li
collection DOAJ
description Proanthocyanidins are natural glycosidase inhibitors with excellent antioxidant activity. This study aims to search for a new source of proanthocyanidins for the prevention and treatment of type 2 diabetes with higher content and better activity and get their structure elucidated. First, the total proanthocyanidins contents (TOPCs), antioxidant activity, antidiabetic activity of seven common Polygonaceae plants were analyzed and compared. Then proanthocyanidins from the rhizome of <i>Fagopyrum dibotrys</i> were purified, and the detailed structure was comprehensively analyzed by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), <sup>13</sup>C nuclear magnetic resonance spectroscopy (<sup>13</sup>C NMR), reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS), and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The rhizome of <i>F. dibotrys</i> showed the highest TOPCs, the strongest antioxidant, and antidiabetic activities; the TOPCs, antioxidant and antidiabetic activities were all very significantly positively correlated. Proanthocyanidins purified from the rhizome of <i>F. dibotrys</i> showed better antidiabetic activity than grape seed proanthocyanidins (GsPs). Seventy-two proanthocyanidins from trimer to undecamer with a mean degree of polymerization (mDP) of about 5.02 ± 0.21 were identified with catechin and epicatechin as the dominant monomers. Conclusion: Proanthocyanidins are the main antioxidant and antidiabetic active substances of <i>F. dibotrys</i> and are expected to be developed into potential antioxidant and hypoglycemic products.
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spelling doaj.art-1c338a670498415ea80297f0e03feb2b2023-11-21T16:30:48ZengMDPI AGMolecules1420-30492021-04-01269241710.3390/molecules26092417Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>Xin Li0Jingling Liu1Qinxiang Chang2Ziyun Zhou3Ruilian Han4Zongsuo Liang5College of Life Sciences, Northwest A & F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest A & F University, Yangling 712100, ChinaInstitute of Landscape, Taiyuan University, Taiyuan 030032, ChinaCollege of Life Sciences, Northwest A & F University, Yangling 712100, ChinaZhejiang Provincial Key Laboratory of Plant Secondary Metabolism Regulation, College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaCollege of Life Sciences, Northwest A & F University, Yangling 712100, ChinaProanthocyanidins are natural glycosidase inhibitors with excellent antioxidant activity. This study aims to search for a new source of proanthocyanidins for the prevention and treatment of type 2 diabetes with higher content and better activity and get their structure elucidated. First, the total proanthocyanidins contents (TOPCs), antioxidant activity, antidiabetic activity of seven common Polygonaceae plants were analyzed and compared. Then proanthocyanidins from the rhizome of <i>Fagopyrum dibotrys</i> were purified, and the detailed structure was comprehensively analyzed by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), <sup>13</sup>C nuclear magnetic resonance spectroscopy (<sup>13</sup>C NMR), reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS), and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The rhizome of <i>F. dibotrys</i> showed the highest TOPCs, the strongest antioxidant, and antidiabetic activities; the TOPCs, antioxidant and antidiabetic activities were all very significantly positively correlated. Proanthocyanidins purified from the rhizome of <i>F. dibotrys</i> showed better antidiabetic activity than grape seed proanthocyanidins (GsPs). Seventy-two proanthocyanidins from trimer to undecamer with a mean degree of polymerization (mDP) of about 5.02 ± 0.21 were identified with catechin and epicatechin as the dominant monomers. Conclusion: Proanthocyanidins are the main antioxidant and antidiabetic active substances of <i>F. dibotrys</i> and are expected to be developed into potential antioxidant and hypoglycemic products.https://www.mdpi.com/1420-3049/26/9/2417<i>Fagopyrum dibotrys</i>proanthocyanidinsantioxidantantidiabetesstructural analysis
spellingShingle Xin Li
Jingling Liu
Qinxiang Chang
Ziyun Zhou
Ruilian Han
Zongsuo Liang
Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
Molecules
<i>Fagopyrum dibotrys</i>
proanthocyanidins
antioxidant
antidiabetes
structural analysis
title Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
title_full Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
title_fullStr Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
title_full_unstemmed Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
title_short Antioxidant and Antidiabetic Activity of Proanthocyanidins from <i>Fagopyrum dibotrys</i>
title_sort antioxidant and antidiabetic activity of proanthocyanidins from i fagopyrum dibotrys i
topic <i>Fagopyrum dibotrys</i>
proanthocyanidins
antioxidant
antidiabetes
structural analysis
url https://www.mdpi.com/1420-3049/26/9/2417
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AT ziyunzhou antioxidantandantidiabeticactivityofproanthocyanidinsfromifagopyrumdibotrysi
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