Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia
Follicular atresia occurs in every stage of ovarian development, which is relevant to female fertility. In the past decade, increasing studies have confirmed that miRNAs, a class of short non-coding RNAs, play an important role in follicular atresia by post-transcription regulation of their target g...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1338411/full |
| _version_ | 1797384927966134272 |
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| author | Jingge Liu Caibo Ning Jinbi Zhang Shiyong Xu Jiege Wu Chenyu Tao Fanhua Ma Qing Chen Zengxiang Pan |
| author_facet | Jingge Liu Caibo Ning Jinbi Zhang Shiyong Xu Jiege Wu Chenyu Tao Fanhua Ma Qing Chen Zengxiang Pan |
| author_sort | Jingge Liu |
| collection | DOAJ |
| description | Follicular atresia occurs in every stage of ovarian development, which is relevant to female fertility. In the past decade, increasing studies have confirmed that miRNAs, a class of short non-coding RNAs, play an important role in follicular atresia by post-transcription regulation of their target genes. However, the function of miRNAs on follicular atresia initiation is unknown. In the present study, high-throughput small RNA sequencing was performed to analyze differential miRNA expression profiles between healthy (HF) follicles and early atretic (EAF) follicles. A total of 237 conserved miRNA were detected, and the miR-143 is the highest expressed in follicles. Meanwhile, we also found wide sequence variations (isomiRs) in porcine ovarian miRNA, including in 5′un-translation region, core seed sequences and 3′untranslation region. Furthermore, we identified 22 differentially expressed miRNAs in EAF groups compared to HF group, of which 3 miRNAs were upregulated, as well as 19 miRNAs were downregulated, and then the RT-PCR was performed to validate these profiles. The target genes of these differentially expressed miRNAs were predicted by using miRwalk, miRDB, and Targetscan database, respectively. Moreover, the gene ontology and KEGG pathway enrichment established that the regulating functions and signaling pathways of these miRNAs contribute to follicular atresia initiation and cell fate. In conclusion, this study provides new insights into the changes of miRNAs in early atretic follicles to demonstrate their molecular regulation in ovarian follicular atretic initiation. |
| first_indexed | 2024-03-08T21:46:45Z |
| format | Article |
| id | doaj.art-1c36a32d18294cac938751fe015ad626 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-08T21:46:45Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-1c36a32d18294cac938751fe015ad6262023-12-20T08:06:41ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-12-011410.3389/fgene.2023.13384111338411Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresiaJingge Liu0Caibo Ning1Jinbi Zhang2Shiyong Xu3Jiege Wu4Chenyu Tao5Fanhua Ma6Qing Chen7Zengxiang Pan8College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, ChinaCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, ChinaFollicular atresia occurs in every stage of ovarian development, which is relevant to female fertility. In the past decade, increasing studies have confirmed that miRNAs, a class of short non-coding RNAs, play an important role in follicular atresia by post-transcription regulation of their target genes. However, the function of miRNAs on follicular atresia initiation is unknown. In the present study, high-throughput small RNA sequencing was performed to analyze differential miRNA expression profiles between healthy (HF) follicles and early atretic (EAF) follicles. A total of 237 conserved miRNA were detected, and the miR-143 is the highest expressed in follicles. Meanwhile, we also found wide sequence variations (isomiRs) in porcine ovarian miRNA, including in 5′un-translation region, core seed sequences and 3′untranslation region. Furthermore, we identified 22 differentially expressed miRNAs in EAF groups compared to HF group, of which 3 miRNAs were upregulated, as well as 19 miRNAs were downregulated, and then the RT-PCR was performed to validate these profiles. The target genes of these differentially expressed miRNAs were predicted by using miRwalk, miRDB, and Targetscan database, respectively. Moreover, the gene ontology and KEGG pathway enrichment established that the regulating functions and signaling pathways of these miRNAs contribute to follicular atresia initiation and cell fate. In conclusion, this study provides new insights into the changes of miRNAs in early atretic follicles to demonstrate their molecular regulation in ovarian follicular atretic initiation.https://www.frontiersin.org/articles/10.3389/fgene.2023.1338411/fullmiRNAspig ovaryfollicle atresia initiationfunction analysissequencing |
| spellingShingle | Jingge Liu Caibo Ning Jinbi Zhang Shiyong Xu Jiege Wu Chenyu Tao Fanhua Ma Qing Chen Zengxiang Pan Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia Frontiers in Genetics miRNAs pig ovary follicle atresia initiation function analysis sequencing |
| title | Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia |
| title_full | Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia |
| title_fullStr | Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia |
| title_full_unstemmed | Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia |
| title_short | Comparative miRNA expression profile analysis of porcine ovarian follicles: new insights into the initiation mechanism of follicular atresia |
| title_sort | comparative mirna expression profile analysis of porcine ovarian follicles new insights into the initiation mechanism of follicular atresia |
| topic | miRNAs pig ovary follicle atresia initiation function analysis sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1338411/full |
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