Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves.
Context: Sage (Salvia officinalis) is an ancient valuable plant used in the treatment of variant health issues. Aims: To evaluate the depigmentation activity of S. officinalis leaf chloroformic (SOCF) and ethanolic (SOMF) extracts via its efficacy to inhibit tyrosinase enzyme using in vitro model...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2022-07-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol10/jppres22.1343_10.4.605.pdf |
| _version_ | 1811312589107363840 |
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| author | Lana Y.M. Juee |
| author_facet | Lana Y.M. Juee |
| author_sort | Lana Y.M. Juee |
| collection | DOAJ |
| description | Context: Sage (Salvia officinalis) is an ancient valuable plant used in the treatment of variant health issues.
Aims: To evaluate the depigmentation activity of S. officinalis leaf chloroformic (SOCF) and ethanolic (SOMF) extracts via its efficacy to inhibit tyrosinase enzyme using in vitro model and bioassay-guided identification and quantification of the main active constituents.
Methods: Plant extracts efficacy as a depigmentation agent has been studied via mushroom tyrosinase inhibition using in vitro model at two concentrations (100 and 200 µg/mL). Extracts were analyzed for phenolic compounds that could be responsible for the biological activity using LC-MS/MS analysis.
Results: Significant potency at a high concentration of 200 µg/mL for the methanolic extract were recorded (p≤0.05). The LC-MS/MS analysis of S. officinalis leaf extracts revealed the presence of eight and fourteen analytes of origin of thirty-seven analytes in both SOCF and SOMF, respectively. Analytes’ quantification recorded the highest amount for rosmarinic acid (46 016 µg/g) in SOMF and the lowest was hesperidin (0.6 µg/g) in SOCF.
Conclusions: S. officinalis extracts recorded significant tyrosinase inhibition potency could control the melanin synthesis process and exhibit beneficiary effect in hyperpigmentation issues. |
| first_indexed | 2024-04-13T10:39:55Z |
| format | Article |
| id | doaj.art-1c3c9969da06471b86cd2128d6e955c9 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-13T10:39:55Z |
| publishDate | 2022-07-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-1c3c9969da06471b86cd2128d6e955c92022-12-22T02:49:57ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502022-07-01104605615 Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves.Lana Y.M. Juee0Department of Pharmacognosy, Pharmacy College, Hawler Medical University, Erbil, Kurdistan Region, Iraq.Context: Sage (Salvia officinalis) is an ancient valuable plant used in the treatment of variant health issues. Aims: To evaluate the depigmentation activity of S. officinalis leaf chloroformic (SOCF) and ethanolic (SOMF) extracts via its efficacy to inhibit tyrosinase enzyme using in vitro model and bioassay-guided identification and quantification of the main active constituents. Methods: Plant extracts efficacy as a depigmentation agent has been studied via mushroom tyrosinase inhibition using in vitro model at two concentrations (100 and 200 µg/mL). Extracts were analyzed for phenolic compounds that could be responsible for the biological activity using LC-MS/MS analysis. Results: Significant potency at a high concentration of 200 µg/mL for the methanolic extract were recorded (p≤0.05). The LC-MS/MS analysis of S. officinalis leaf extracts revealed the presence of eight and fourteen analytes of origin of thirty-seven analytes in both SOCF and SOMF, respectively. Analytes’ quantification recorded the highest amount for rosmarinic acid (46 016 µg/g) in SOMF and the lowest was hesperidin (0.6 µg/g) in SOCF. Conclusions: S. officinalis extracts recorded significant tyrosinase inhibition potency could control the melanin synthesis process and exhibit beneficiary effect in hyperpigmentation issues.https://jppres.com/jppres/pdf/vol10/jppres22.1343_10.4.605.pdfhesperidinhyperpigmentationlc-ms/ms spectroscopyrosmarinic acidsagetyrosinase inhibitors |
| spellingShingle | Lana Y.M. Juee Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. Journal of Pharmacy & Pharmacognosy Research hesperidin hyperpigmentation lc-ms/ms spectroscopy rosmarinic acid sage tyrosinase inhibitors |
| title | Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. |
| title_full | Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. |
| title_fullStr | Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. |
| title_full_unstemmed | Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. |
| title_short | Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. |
| title_sort | phytochemical characterization and mushroom tyrosinase inhibition of different extracts from salvia officinalis l leaves |
| topic | hesperidin hyperpigmentation lc-ms/ms spectroscopy rosmarinic acid sage tyrosinase inhibitors |
| url | https://jppres.com/jppres/pdf/vol10/jppres22.1343_10.4.605.pdf |
| work_keys_str_mv | AT lanaymjuee phytochemicalcharacterizationandmushroomtyrosinaseinhibitionofdifferentextractsfromsalviaofficinalislleaves |