Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis
Streptococcus suis (S. suis), a major swine pathogen, is also a severe threat to human health. Infection with highly virulent strains of S. suis can cause human Streptococcal toxic-shock-like syndrome (STSLS), which is associated with high serum pro-inflammatory cytokine levels and a high mortality...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00079/full |
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| author | Chao eYang Jianqing eZhao Lan eLin Shan ePan Lei eFu Li eHan Meilin eJin Rui eZhou Anding eZhang |
| author_facet | Chao eYang Jianqing eZhao Lan eLin Shan ePan Lei eFu Li eHan Meilin eJin Rui eZhou Anding eZhang |
| author_sort | Chao eYang |
| collection | DOAJ |
| description | Streptococcus suis (S. suis), a major swine pathogen, is also a severe threat to human health. Infection with highly virulent strains of S. suis can cause human Streptococcal toxic-shock-like syndrome (STSLS), which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. Our previous study indicated that highly virulent S. suis infection could activate the TREM-1 signaling pathway, which promotes host clearance of S. suis during early infection. However, it remained to be elicited whether TREM-1 signaling could be a target against STSLS in the presence of antibiotic. In the present study, mice were infected with a highly virulent S. suis strain and then treated with rTREM-1 (the recombinant extracellular domain of TREM-1) to block TREM-1 signaling, antibiotics, both rTREM-1 and antibiotics, or PBS. The survival rates, clinical signs, serum IL-1β and TNF-α levels, and serum bacterial loads were evaluated. Treatment with rTREM-1 could aggravate the outcome of infection as described previously. Although the conventional treatment with antibiotics contributed to effective S. suis clearance, it did not improve survival significantly. In comparison, due to the reduction of the exaggerated pro-inflammatory response, treatment combined with rTREM-1 and antibiotics not only led to efficient bacterial clearance but also alleviated inflammation. In conclusion, TREM-1 signaling contributed to severe inflammatory response and benefited S. suis clearance. Therefore, blocking TREM-1 signaling could still be a target for the treatment of STSLS in the presence of antibiotics. |
| first_indexed | 2024-12-23T20:32:50Z |
| format | Article |
| id | doaj.art-1c3d6f2e32a142c48de8fb8eefdce444 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-23T20:32:50Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-1c3d6f2e32a142c48de8fb8eefdce4442022-12-21T17:32:10ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882015-11-01510.3389/fcimb.2015.00079167683Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suisChao eYang0Jianqing eZhao1Lan eLin2Shan ePan3Lei eFu4Li eHan5Meilin eJin6Rui eZhou7Anding eZhang8Huazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityStreptococcus suis (S. suis), a major swine pathogen, is also a severe threat to human health. Infection with highly virulent strains of S. suis can cause human Streptococcal toxic-shock-like syndrome (STSLS), which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. Our previous study indicated that highly virulent S. suis infection could activate the TREM-1 signaling pathway, which promotes host clearance of S. suis during early infection. However, it remained to be elicited whether TREM-1 signaling could be a target against STSLS in the presence of antibiotic. In the present study, mice were infected with a highly virulent S. suis strain and then treated with rTREM-1 (the recombinant extracellular domain of TREM-1) to block TREM-1 signaling, antibiotics, both rTREM-1 and antibiotics, or PBS. The survival rates, clinical signs, serum IL-1β and TNF-α levels, and serum bacterial loads were evaluated. Treatment with rTREM-1 could aggravate the outcome of infection as described previously. Although the conventional treatment with antibiotics contributed to effective S. suis clearance, it did not improve survival significantly. In comparison, due to the reduction of the exaggerated pro-inflammatory response, treatment combined with rTREM-1 and antibiotics not only led to efficient bacterial clearance but also alleviated inflammation. In conclusion, TREM-1 signaling contributed to severe inflammatory response and benefited S. suis clearance. Therefore, blocking TREM-1 signaling could still be a target for the treatment of STSLS in the presence of antibiotics.http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00079/fullAnti-Inflammatory Agentstreatment targetsTriggering receptor expressed on myeloid cells-1 (TREM-1)Streptococcus suis (S. suis)Streptococcal toxic-shock-like syndrome (STSLS) |
| spellingShingle | Chao eYang Jianqing eZhao Lan eLin Shan ePan Lei eFu Li eHan Meilin eJin Rui eZhou Anding eZhang Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis Frontiers in Cellular and Infection Microbiology Anti-Inflammatory Agents treatment targets Triggering receptor expressed on myeloid cells-1 (TREM-1) Streptococcus suis (S. suis) Streptococcal toxic-shock-like syndrome (STSLS) |
| title | Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis |
| title_full | Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis |
| title_fullStr | Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis |
| title_full_unstemmed | Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis |
| title_short | Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS) caused by Streptococcus suis |
| title_sort | targeting trem 1 signaling in the presence of antibiotics is effective against streptococcal toxic shock like syndrome stsls caused by streptococcus suis |
| topic | Anti-Inflammatory Agents treatment targets Triggering receptor expressed on myeloid cells-1 (TREM-1) Streptococcus suis (S. suis) Streptococcal toxic-shock-like syndrome (STSLS) |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00079/full |
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