Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties

The study and use of non-<i>Saccharomyces</i> yeasts to wine improvement and diversification has gained considerable relevance in recent years. The present work reports a pilot-scale winery assay of mixed fermentation with a commercial strain of <i>Metschnikowia pulcherrima</i&g...

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Main Authors: Filomena L. Duarte, Ricardo Egipto, M. Margarida Baleiras-Couto
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Fermentation
Subjects:
Online Access:https://www.mdpi.com/2311-5637/5/3/59
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author Filomena L. Duarte
Ricardo Egipto
M. Margarida Baleiras-Couto
author_facet Filomena L. Duarte
Ricardo Egipto
M. Margarida Baleiras-Couto
author_sort Filomena L. Duarte
collection DOAJ
description The study and use of non-<i>Saccharomyces</i> yeasts to wine improvement and diversification has gained considerable relevance in recent years. The present work reports a pilot-scale winery assay of mixed fermentation with a commercial strain of <i>Metschnikowia pulcherrima</i>, tested in five white and nine red grape varieties. Two modalities were assayed, one with the addition of <i>M. pulcherrima</i> at time zero and addition of <i>Saccharomyces cerevisiae</i> after 24 h, and a control using only <i>S. cerevisiae</i> at time zero. Fermentation was monitored by daily measurement of density and temperature. Wine physicochemical analysis was performed after winemaking and repeated after four years of aging. Variance and multivariate analysis were used to examine these data. Triangle and ranking tests were performed on the wines obtained, using an experienced sensory panel. Alcoholic fermentation proceeded smoothly until there was complete consumption of the sugars. <i>M. pulcherrima</i> in mixed fermentation, although mainly recommended for white wine, was also tested for red wines. These wines generally presented higher glycerol, reducing sugars and total dry matter, and lower alcohol content, in line with the current market trend. Significant sensory differences among modalities were only obtained for three varieties. Results emphasized that grape variety is a relevant factor in studies with non-<i>Saccharomyces</i> yeasts.
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spelling doaj.art-1c4063fbadba416c9881857ece10ada62022-12-22T03:08:00ZengMDPI AGFermentation2311-56372019-07-01535910.3390/fermentation5030059fermentation5030059Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape VarietiesFilomena L. Duarte0Ricardo Egipto1M. Margarida Baleiras-Couto2Instituto Nacional de Investigação Agrária e Veterinária, INIAV-Dois Portos, Quinta da Almoínha, 2565-191 Dois Portos, PortugalInstituto Nacional de Investigação Agrária e Veterinária, INIAV-Dois Portos, Quinta da Almoínha, 2565-191 Dois Portos, PortugalInstituto Nacional de Investigação Agrária e Veterinária, INIAV-Dois Portos, Quinta da Almoínha, 2565-191 Dois Portos, PortugalThe study and use of non-<i>Saccharomyces</i> yeasts to wine improvement and diversification has gained considerable relevance in recent years. The present work reports a pilot-scale winery assay of mixed fermentation with a commercial strain of <i>Metschnikowia pulcherrima</i>, tested in five white and nine red grape varieties. Two modalities were assayed, one with the addition of <i>M. pulcherrima</i> at time zero and addition of <i>Saccharomyces cerevisiae</i> after 24 h, and a control using only <i>S. cerevisiae</i> at time zero. Fermentation was monitored by daily measurement of density and temperature. Wine physicochemical analysis was performed after winemaking and repeated after four years of aging. Variance and multivariate analysis were used to examine these data. Triangle and ranking tests were performed on the wines obtained, using an experienced sensory panel. Alcoholic fermentation proceeded smoothly until there was complete consumption of the sugars. <i>M. pulcherrima</i> in mixed fermentation, although mainly recommended for white wine, was also tested for red wines. These wines generally presented higher glycerol, reducing sugars and total dry matter, and lower alcohol content, in line with the current market trend. Significant sensory differences among modalities were only obtained for three varieties. Results emphasized that grape variety is a relevant factor in studies with non-<i>Saccharomyces</i> yeasts.https://www.mdpi.com/2311-5637/5/3/59mixed fermentations<i>Metschnikowia pulcherrima</i>winegrape varietysensory analysis
spellingShingle Filomena L. Duarte
Ricardo Egipto
M. Margarida Baleiras-Couto
Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
Fermentation
mixed fermentations
<i>Metschnikowia pulcherrima</i>
wine
grape variety
sensory analysis
title Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
title_full Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
title_fullStr Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
title_full_unstemmed Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
title_short Mixed Fermentation with <i>Metschnikowia pulcherrima</i> Using Different Grape Varieties
title_sort mixed fermentation with i metschnikowia pulcherrima i using different grape varieties
topic mixed fermentations
<i>Metschnikowia pulcherrima</i>
wine
grape variety
sensory analysis
url https://www.mdpi.com/2311-5637/5/3/59
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AT ricardoegipto mixedfermentationwithimetschnikowiapulcherrimaiusingdifferentgrapevarieties
AT mmargaridabaleirascouto mixedfermentationwithimetschnikowiapulcherrimaiusingdifferentgrapevarieties