Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials
Profitable biomass conversion processes are highly dependent on the use of efficient enzymes for lignocellulose degradation. Among the cellulose degrading enzymes, beta-glucosidases are essential for efficient hydrolysis of cellulosic biomass as they relieve the inhibition of the cellobiohydrolases...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-09-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2218-273X/3/3/612 |
| _version_ | 1811325396643217408 |
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| author | Peter S. Lübeck Birgitte K. Ahring Mette Lübeck Annette Sørensen |
| author_facet | Peter S. Lübeck Birgitte K. Ahring Mette Lübeck Annette Sørensen |
| author_sort | Peter S. Lübeck |
| collection | DOAJ |
| description | Profitable biomass conversion processes are highly dependent on the use of efficient enzymes for lignocellulose degradation. Among the cellulose degrading enzymes, beta-glucosidases are essential for efficient hydrolysis of cellulosic biomass as they relieve the inhibition of the cellobiohydrolases and endoglucanases by reducing cellobiose accumulation. In this review, we discuss the important role beta-glucosidases play in complex biomass hydrolysis and how they create a bottleneck in industrial use of lignocellulosic materials. An efficient beta-glucosidase facilitates hydrolysis at specified process conditions, and key points to consider in this respect are hydrolysis rate, inhibitors, and stability. Product inhibition impairing yields, thermal inactivation of enzymes, and the high cost of enzyme production are the main obstacles to commercial cellulose hydrolysis. Therefore, this sets the stage in the search for better alternatives to the currently available enzyme preparations either by improving known or screening for new beta-glucosidases. |
| first_indexed | 2024-04-13T14:33:30Z |
| format | Article |
| id | doaj.art-1c41d9697be34deeb588485b77bbaa1a |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-13T14:33:30Z |
| publishDate | 2013-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1c41d9697be34deeb588485b77bbaa1a2022-12-22T02:43:07ZengMDPI AGBiomolecules2218-273X2013-09-013361263110.3390/biom3030612Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic MaterialsPeter S. LübeckBirgitte K. AhringMette LübeckAnnette SørensenProfitable biomass conversion processes are highly dependent on the use of efficient enzymes for lignocellulose degradation. Among the cellulose degrading enzymes, beta-glucosidases are essential for efficient hydrolysis of cellulosic biomass as they relieve the inhibition of the cellobiohydrolases and endoglucanases by reducing cellobiose accumulation. In this review, we discuss the important role beta-glucosidases play in complex biomass hydrolysis and how they create a bottleneck in industrial use of lignocellulosic materials. An efficient beta-glucosidase facilitates hydrolysis at specified process conditions, and key points to consider in this respect are hydrolysis rate, inhibitors, and stability. Product inhibition impairing yields, thermal inactivation of enzymes, and the high cost of enzyme production are the main obstacles to commercial cellulose hydrolysis. Therefore, this sets the stage in the search for better alternatives to the currently available enzyme preparations either by improving known or screening for new beta-glucosidases.http://www.mdpi.com/2218-273X/3/3/612beta-glucosidasecellulasebiomasshydrolysisbiofuels |
| spellingShingle | Peter S. Lübeck Birgitte K. Ahring Mette Lübeck Annette Sørensen Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials Biomolecules beta-glucosidase cellulase biomass hydrolysis biofuels |
| title | Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials |
| title_full | Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials |
| title_fullStr | Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials |
| title_full_unstemmed | Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials |
| title_short | Fungal Beta-Glucosidases: A Bottleneck in Industrial Use of Lignocellulosic Materials |
| title_sort | fungal beta glucosidases a bottleneck in industrial use of lignocellulosic materials |
| topic | beta-glucosidase cellulase biomass hydrolysis biofuels |
| url | http://www.mdpi.com/2218-273X/3/3/612 |
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