Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus
Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenot...
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| Format: | Article |
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Elsevier
2014-12-01
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| Series: | Biomedical Journal |
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| Online Access: | http://www.biomedj.org/article.asp?issn=2319-4170;year=2014;volume=37;issue=6;spage=411;epage=414;aulast=Kali |
| _version_ | 1817991824888823808 |
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| author | Arunava Kali Selvaraj Stephen Sivaraman Umadevi |
| author_facet | Arunava Kali Selvaraj Stephen Sivaraman Umadevi |
| author_sort | Arunava Kali |
| collection | DOAJ |
| description | Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred and two clinical isolates of MRSA identified by the oxacillin disk diffusion were subjected to PCR for the mecA gene and by the cefoxitin disk diffusion test and culture on oxacillin screen agar, mannitol salt agar, and methicillin-resistant Staphylococcus aureus Agar (MeReSA) selective medium, for MRSA. Although all 102 isolates were resistant in oxacillin and cefoxitin disk diffusion, 92 (90.1%) isolates were positive for the mecA gene. The sensitivities of the mannitol salt agar, MeReSA agar, and oxacillin screen agar were 89.13, 97.82, and 98.91%, respectively. The oxacillin screen agar may be recommended for confirming methicillin resistance in the disk diffusion test in resource-poor settings, where molecular methods are not available. |
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| format | Article |
| id | doaj.art-1c440e96e64b482986906462039e5c5c |
| institution | Directory Open Access Journal |
| issn | 2319-4170 2320-2890 |
| language | English |
| last_indexed | 2024-04-14T01:18:25Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier |
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| series | Biomedical Journal |
| spelling | doaj.art-1c440e96e64b482986906462039e5c5c2022-12-22T02:20:45ZengElsevierBiomedical Journal2319-41702320-28902014-12-0137641141410.4103/2319-4170.132907Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureusArunava Kali0Selvaraj Stephen1Sivaraman Umadevi2Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry, IndiaDepartment of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry, IndiaDepartment of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry, IndiaAlthough conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred and two clinical isolates of MRSA identified by the oxacillin disk diffusion were subjected to PCR for the mecA gene and by the cefoxitin disk diffusion test and culture on oxacillin screen agar, mannitol salt agar, and methicillin-resistant Staphylococcus aureus Agar (MeReSA) selective medium, for MRSA. Although all 102 isolates were resistant in oxacillin and cefoxitin disk diffusion, 92 (90.1%) isolates were positive for the mecA gene. The sensitivities of the mannitol salt agar, MeReSA agar, and oxacillin screen agar were 89.13, 97.82, and 98.91%, respectively. The oxacillin screen agar may be recommended for confirming methicillin resistance in the disk diffusion test in resource-poor settings, where molecular methods are not available.http://www.biomedj.org/article.asp?issn=2319-4170;year=2014;volume=37;issue=6;spage=411;epage=414;aulast=KaliCefoxitin disk diffusionmethicillin-resistant Staphylococcus aureusmecA geneoxacillin disk diffusionoxacillin screen agarpolymerase chain reaction |
| spellingShingle | Arunava Kali Selvaraj Stephen Sivaraman Umadevi Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus Biomedical Journal Cefoxitin disk diffusion methicillin-resistant Staphylococcus aureus mecA gene oxacillin disk diffusion oxacillin screen agar polymerase chain reaction |
| title | Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus |
| title_full | Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus |
| title_fullStr | Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus |
| title_short | Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus |
| title_sort | laboratory evaluation of phenotypic detection methods of methicillin resistant staphylococcus aureus |
| topic | Cefoxitin disk diffusion methicillin-resistant Staphylococcus aureus mecA gene oxacillin disk diffusion oxacillin screen agar polymerase chain reaction |
| url | http://www.biomedj.org/article.asp?issn=2319-4170;year=2014;volume=37;issue=6;spage=411;epage=414;aulast=Kali |
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