Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle
Interferon-induced transmembrane protein 3 (IFITM3), a crucial effector of the host’s innate immune system, prohibits an extensive range of viruses. Previous studies have reported that single nucleotide polymorphisms (SNPs) of the <i>IFITM3</i> gene are associated with the expression lev...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2073-4425/12/11/1662 |
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| author | Yong-Chan Kim Min-Ju Jeong Byung-Hoon Jeong |
| author_facet | Yong-Chan Kim Min-Ju Jeong Byung-Hoon Jeong |
| author_sort | Yong-Chan Kim |
| collection | DOAJ |
| description | Interferon-induced transmembrane protein 3 (IFITM3), a crucial effector of the host’s innate immune system, prohibits an extensive range of viruses. Previous studies have reported that single nucleotide polymorphisms (SNPs) of the <i>IFITM3</i> gene are associated with the expression level and length of the IFITM3 protein and can impact susceptibility to infectious viruses and the severity of infection with these viruses. However, there have been no studies on polymorphisms of the bovine <i>IFITM3</i> gene. In the present study, we finely mapped the bovine <i>IFITM3</i> gene and annotated the identified polymorphisms. We investigated polymorphisms of the bovine <i>IFITM3</i> gene in 108 Hanwoo and 113 Holstein cattle using direct sequencing and analyzed genotype, allele, and haplotype frequencies and linkage disequilibrium (LD) between the <i>IFITM3</i> genes of the two cattle breeds. In addition, we analyzed transcription factor-binding sites and transcriptional capacity using PROMO and luciferase assays, respectively. Furthermore, we analyzed the effect of a nonsynonymous SNP of the <i>IFITM3</i> gene using PolyPhen-2, PANTHER, and PROVEAN. We identified 23 polymorphisms in the bovine <i>IFITM3</i> gene and found significantly different genotype, allele, and haplotype frequency distributions and LD scores between polymorphisms of the bovine <i>IFITM3</i> gene in Hanwoo and Holstein cattle. In addition, the ability to bind the transcription factor Nkx2-1 and transcriptional capacities were significantly different depending on the c.-193T > C allele. Furthermore, nonsynonymous SNP (F121L) was predicted to be benign. To the best of our knowledge, this is the first genetic study of bovine <i>IFITM3</i> polymorphisms. |
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| language | English |
| last_indexed | 2024-03-10T05:28:16Z |
| publishDate | 2021-10-01 |
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| series | Genes |
| spelling | doaj.art-1c46fb6827e74ebf9c36d4be6b7bf1802023-11-22T23:26:57ZengMDPI AGGenes2073-44252021-10-011211166210.3390/genes12111662Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein CattleYong-Chan Kim0Min-Ju Jeong1Byung-Hoon Jeong2Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, KoreaKorea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, KoreaKorea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, KoreaInterferon-induced transmembrane protein 3 (IFITM3), a crucial effector of the host’s innate immune system, prohibits an extensive range of viruses. Previous studies have reported that single nucleotide polymorphisms (SNPs) of the <i>IFITM3</i> gene are associated with the expression level and length of the IFITM3 protein and can impact susceptibility to infectious viruses and the severity of infection with these viruses. However, there have been no studies on polymorphisms of the bovine <i>IFITM3</i> gene. In the present study, we finely mapped the bovine <i>IFITM3</i> gene and annotated the identified polymorphisms. We investigated polymorphisms of the bovine <i>IFITM3</i> gene in 108 Hanwoo and 113 Holstein cattle using direct sequencing and analyzed genotype, allele, and haplotype frequencies and linkage disequilibrium (LD) between the <i>IFITM3</i> genes of the two cattle breeds. In addition, we analyzed transcription factor-binding sites and transcriptional capacity using PROMO and luciferase assays, respectively. Furthermore, we analyzed the effect of a nonsynonymous SNP of the <i>IFITM3</i> gene using PolyPhen-2, PANTHER, and PROVEAN. We identified 23 polymorphisms in the bovine <i>IFITM3</i> gene and found significantly different genotype, allele, and haplotype frequency distributions and LD scores between polymorphisms of the bovine <i>IFITM3</i> gene in Hanwoo and Holstein cattle. In addition, the ability to bind the transcription factor Nkx2-1 and transcriptional capacities were significantly different depending on the c.-193T > C allele. Furthermore, nonsynonymous SNP (F121L) was predicted to be benign. To the best of our knowledge, this is the first genetic study of bovine <i>IFITM3</i> polymorphisms.https://www.mdpi.com/2073-4425/12/11/1662cattleIFITM3FMDVIAVSNPpromoter |
| spellingShingle | Yong-Chan Kim Min-Ju Jeong Byung-Hoon Jeong Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle Genes cattle IFITM3 FMDV IAV SNP promoter |
| title | Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle |
| title_full | Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle |
| title_fullStr | Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle |
| title_full_unstemmed | Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle |
| title_short | Regulatory Single Nucleotide Polymorphism of the Bovine <i>IFITM3</i> Gene Induces Differential Transcriptional Capacities of Hanwoo and Holstein Cattle |
| title_sort | regulatory single nucleotide polymorphism of the bovine i ifitm3 i gene induces differential transcriptional capacities of hanwoo and holstein cattle |
| topic | cattle IFITM3 FMDV IAV SNP promoter |
| url | https://www.mdpi.com/2073-4425/12/11/1662 |
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