Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions
Nitric oxide (NO) is a signaling molecule that participates in plant adaptation to adverse environmental factors. This study aimed to clarify the role of NO in the regulation of vacuolar H<sup>+</sup>-ATPase (V-ATPase) in the roots of cucumber seedlings grown under control and Cd stress...
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MDPI AG
2023-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/15/2884 |
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| author | Magdalena Zboińska Anna Janeczko Katarzyna Kabała |
| author_facet | Magdalena Zboińska Anna Janeczko Katarzyna Kabała |
| author_sort | Magdalena Zboińska |
| collection | DOAJ |
| description | Nitric oxide (NO) is a signaling molecule that participates in plant adaptation to adverse environmental factors. This study aimed to clarify the role of NO in the regulation of vacuolar H<sup>+</sup>-ATPase (V-ATPase) in the roots of cucumber seedlings grown under control and Cd stress conditions. In addition, the relationship between NO and salicylic acid (SA), as well as their interrelations with hydrogen sulfide (H<sub>2</sub>S) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), have been verified. The effect of NO on V-ATPase was studied by analyzing two enzyme activities, the expression level of selected <i>VHA</i> genes and the protein level of selected VHA subunits in plants treated with a NO donor (sodium nitroprusside, SNP) and NO biosynthesis inhibitors (tungstate, WO<sub>4</sub><sup>2</sup><b><sup>−</sup></b> and N-nitro-L-arginine methyl ester, L-NAME). Our results indicate that NO functions as a positive regulator of V-ATPase and that this regulation depends on NO generated by nitrate reductase and NOS-like activity. It was found that the mechanism of NO action is not related to changes in the gene expression or protein level of the V-ATPase subunits. The results suggest that in cucumber roots, NO signaling interacts with the SA pathway and, to a lesser extent, with two other known V-ATPase regulators, H<sub>2</sub>O<sub>2</sub> and H<sub>2</sub>S. |
| first_indexed | 2024-03-11T00:18:07Z |
| format | Article |
| id | doaj.art-1c489192ac734c92adc0e2d98894021d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:18:07Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1c489192ac734c92adc0e2d98894021d2023-11-18T23:27:10ZengMDPI AGPlants2223-77472023-08-011215288410.3390/plants12152884Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress ConditionsMagdalena Zboińska0Anna Janeczko1Katarzyna Kabała2Department of Plant Molecular Physiology, Faculty of Biological Sciences, University of Wrocław, Kanonia 6/8, 50-328 Wrocław, PolandThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, PolandDepartment of Plant Molecular Physiology, Faculty of Biological Sciences, University of Wrocław, Kanonia 6/8, 50-328 Wrocław, PolandNitric oxide (NO) is a signaling molecule that participates in plant adaptation to adverse environmental factors. This study aimed to clarify the role of NO in the regulation of vacuolar H<sup>+</sup>-ATPase (V-ATPase) in the roots of cucumber seedlings grown under control and Cd stress conditions. In addition, the relationship between NO and salicylic acid (SA), as well as their interrelations with hydrogen sulfide (H<sub>2</sub>S) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), have been verified. The effect of NO on V-ATPase was studied by analyzing two enzyme activities, the expression level of selected <i>VHA</i> genes and the protein level of selected VHA subunits in plants treated with a NO donor (sodium nitroprusside, SNP) and NO biosynthesis inhibitors (tungstate, WO<sub>4</sub><sup>2</sup><b><sup>−</sup></b> and N-nitro-L-arginine methyl ester, L-NAME). Our results indicate that NO functions as a positive regulator of V-ATPase and that this regulation depends on NO generated by nitrate reductase and NOS-like activity. It was found that the mechanism of NO action is not related to changes in the gene expression or protein level of the V-ATPase subunits. The results suggest that in cucumber roots, NO signaling interacts with the SA pathway and, to a lesser extent, with two other known V-ATPase regulators, H<sub>2</sub>O<sub>2</sub> and H<sub>2</sub>S.https://www.mdpi.com/2223-7747/12/15/2884cadmiumhydrogen peroxidehydrogen sulfidenitric oxidenitrate reductasesalicylic acid |
| spellingShingle | Magdalena Zboińska Anna Janeczko Katarzyna Kabała Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions Plants cadmium hydrogen peroxide hydrogen sulfide nitric oxide nitrate reductase salicylic acid |
| title | Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions |
| title_full | Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions |
| title_fullStr | Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions |
| title_full_unstemmed | Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions |
| title_short | Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions |
| title_sort | involvement of no in v atpase regulation in cucumber roots under control and cadmium stress conditions |
| topic | cadmium hydrogen peroxide hydrogen sulfide nitric oxide nitrate reductase salicylic acid |
| url | https://www.mdpi.com/2223-7747/12/15/2884 |
| work_keys_str_mv | AT magdalenazboinska involvementofnoinvatpaseregulationincucumberrootsundercontrolandcadmiumstressconditions AT annajaneczko involvementofnoinvatpaseregulationincucumberrootsundercontrolandcadmiumstressconditions AT katarzynakabała involvementofnoinvatpaseregulationincucumberrootsundercontrolandcadmiumstressconditions |