Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope
Apoptosis is an essential biological function required during embryogenesis, tissue homeostasis, organ development and immune system regulation. It is an active cell death pathway involved in a variety of pathological conditions. During this process cytoskeletal proteins appear damaged and undergo a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2015-09-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2539 |
| _version_ | 1818156067090071552 |
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| author | S. Salucci S. Burattini E. Falcieri P. Gobbi |
| author_facet | S. Salucci S. Burattini E. Falcieri P. Gobbi |
| author_sort | S. Salucci |
| collection | DOAJ |
| description | Apoptosis is an essential biological function required during embryogenesis, tissue homeostasis, organ development and immune system regulation. It is an active cell death pathway involved in a variety of pathological conditions. During this process cytoskeletal proteins appear damaged and undergo an enzymatic disassembling, leading to formation of apoptotic features. This study was designed to examine the three-dimensional chromatin behavior and cytoskeleton involvement, in particular actin re-modeling. HL-60 cells, exposed to hyperthermia, a known apoptotic trigger, were examined by means of a Field Emission in Lens Scanning Electron Microscope (FEISEM). Ultrastructural observations revealed in treated cells the presence of apoptotic patterns after hyperthermia trigger. In particular, three-dimensional apoptotic chromatin rearrangements appeared involving the translocation of filamentous actin from cytoplasm to the nucleus. FEISEM immunogold techniques showed actin labeling and its precise three-dimensional localization in the diffuse chromatin, well separated from the condensed one. The actin presence in dispersed chromatin inside the apoptotic nucleus can be considered an important feature, indispensable to permit the apoptotic machinery evolution. |
| first_indexed | 2024-12-11T14:52:24Z |
| format | Article |
| id | doaj.art-1c49942a78df4676ae5659278dad6e32 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T14:52:24Z |
| publishDate | 2015-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-1c49942a78df4676ae5659278dad6e322022-12-22T01:01:25ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062015-09-0159310.4081/ejh.2015.25391469Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron MicroscopeS. Salucci0S. Burattini1E. Falcieri2P. Gobbi3University of Urbino Carlo BoUniversity of Urbino Carlo BoUniversity of Urbino Carlo BoUniversity of Urbino Carlo BoApoptosis is an essential biological function required during embryogenesis, tissue homeostasis, organ development and immune system regulation. It is an active cell death pathway involved in a variety of pathological conditions. During this process cytoskeletal proteins appear damaged and undergo an enzymatic disassembling, leading to formation of apoptotic features. This study was designed to examine the three-dimensional chromatin behavior and cytoskeleton involvement, in particular actin re-modeling. HL-60 cells, exposed to hyperthermia, a known apoptotic trigger, were examined by means of a Field Emission in Lens Scanning Electron Microscope (FEISEM). Ultrastructural observations revealed in treated cells the presence of apoptotic patterns after hyperthermia trigger. In particular, three-dimensional apoptotic chromatin rearrangements appeared involving the translocation of filamentous actin from cytoplasm to the nucleus. FEISEM immunogold techniques showed actin labeling and its precise three-dimensional localization in the diffuse chromatin, well separated from the condensed one. The actin presence in dispersed chromatin inside the apoptotic nucleus can be considered an important feature, indispensable to permit the apoptotic machinery evolution.http://www.ejh.it/index.php/ejh/article/view/2539ChromatinF-actinimmunogoldTEMFEISEM. |
| spellingShingle | S. Salucci S. Burattini E. Falcieri P. Gobbi Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope European Journal of Histochemistry Chromatin F-actin immunogold TEM FEISEM. |
| title | Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope |
| title_full | Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope |
| title_fullStr | Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope |
| title_full_unstemmed | Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope |
| title_short | Three-dimensional apoptotic nuclear behavior analyzed by means of Field Emission in Lens Scanning Electron Microscope |
| title_sort | three dimensional apoptotic nuclear behavior analyzed by means of field emission in lens scanning electron microscope |
| topic | Chromatin F-actin immunogold TEM FEISEM. |
| url | http://www.ejh.it/index.php/ejh/article/view/2539 |
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