Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains
Vermicomposting is a cost-effective biotechnology for the management of organic wastes that relies on the activity of earthworms and their associated microbiota. Here, the microbiotas of the earthworm Eisenia fetida fed with brewers’ spent grains (FBSG), cow manure (FCM) and a mix of brewers’ spent...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-03-01
|
| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19306976 |
| _version_ | 1818486473436954624 |
|---|---|
| author | Marilena Budroni Ilaria Mannazzu Severino Zara Sara Saba Antonio Pais Giacomo Zara |
| author_facet | Marilena Budroni Ilaria Mannazzu Severino Zara Sara Saba Antonio Pais Giacomo Zara |
| author_sort | Marilena Budroni |
| collection | DOAJ |
| description | Vermicomposting is a cost-effective biotechnology for the management of organic wastes that relies on the activity of earthworms and their associated microbiota. Here, the microbiotas of the earthworm Eisenia fetida fed with brewers’ spent grains (FBSG), cow manure (FCM) and a mix of brewers’ spent grains/cow manure (FMIX), were identified by high-throughput DNA sequencing (16S rRNA). Bacterial community variance was correlated with the pH and the organic carbon content of the rearing substrates. FBSG microbiota was enriched in Paenibacillaceae, Enterobacteriaceae, Chitinophagaceae and Comamonadaceae. In addition, FBSG microbiota had a predicted higher abundance of genes involved in cellulose degradation as well as in the nitrogen cycle and showed higher utilization of ammonia and nitrate. Results obtained will allow to optimize the vermicomposting of brewers’ spent grains and to evaluate the effect of vermicompost addition on nutrient dynamics in soil. Keywords: Brewers’ spent grains, Phylogeny-based metabolic interference, Canonical correspondence analysis, LEfSe, Biolog |
| first_indexed | 2024-12-10T16:23:29Z |
| format | Article |
| id | doaj.art-1c49b30ce0b8403f9daf1645aeae19b9 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-10T16:23:29Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-1c49b30ce0b8403f9daf1645aeae19b92022-12-22T01:41:44ZengElsevierBiotechnology Reports2215-017X2020-03-0125Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grainsMarilena Budroni0Ilaria Mannazzu1Severino Zara2Sara Saba3Antonio Pais4Giacomo Zara5Department of Agriculture, University of Sassari, 07100, Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100, Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100, Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100, Sassari, ItalyDepartment of Agriculture, University of Sassari, 07100, Sassari, ItalyCorresponding author.; Department of Agriculture, University of Sassari, 07100, Sassari, ItalyVermicomposting is a cost-effective biotechnology for the management of organic wastes that relies on the activity of earthworms and their associated microbiota. Here, the microbiotas of the earthworm Eisenia fetida fed with brewers’ spent grains (FBSG), cow manure (FCM) and a mix of brewers’ spent grains/cow manure (FMIX), were identified by high-throughput DNA sequencing (16S rRNA). Bacterial community variance was correlated with the pH and the organic carbon content of the rearing substrates. FBSG microbiota was enriched in Paenibacillaceae, Enterobacteriaceae, Chitinophagaceae and Comamonadaceae. In addition, FBSG microbiota had a predicted higher abundance of genes involved in cellulose degradation as well as in the nitrogen cycle and showed higher utilization of ammonia and nitrate. Results obtained will allow to optimize the vermicomposting of brewers’ spent grains and to evaluate the effect of vermicompost addition on nutrient dynamics in soil. Keywords: Brewers’ spent grains, Phylogeny-based metabolic interference, Canonical correspondence analysis, LEfSe, Biologhttp://www.sciencedirect.com/science/article/pii/S2215017X19306976 |
| spellingShingle | Marilena Budroni Ilaria Mannazzu Severino Zara Sara Saba Antonio Pais Giacomo Zara Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains Biotechnology Reports |
| title | Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains |
| title_full | Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains |
| title_fullStr | Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains |
| title_full_unstemmed | Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains |
| title_short | Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains |
| title_sort | composition and functional profiling of the microbiota in the casts of eisenia fetida during vermicomposting of brewers spent grains |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X19306976 |
| work_keys_str_mv | AT marilenabudroni compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains AT ilariamannazzu compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains AT severinozara compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains AT sarasaba compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains AT antoniopais compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains AT giacomozara compositionandfunctionalprofilingofthemicrobiotainthecastsofeiseniafetidaduringvermicompostingofbrewersspentgrains |