Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump
In the present study genotypic resistance of bacterial strain to first- and second-line anti-tuberculosis drugs was determined by Line probe assay. Toxic effect of tellurite on the growth of Mycobacterium tuberculosis (MTB) was determined by growing cells in different concentrations of tellurite (1...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723000915 |
| _version_ | 1797844310108930048 |
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| author | Saba Kabir Hasan Ejaz Syed Zajif Hussain Muhammad Asif Rasheed Kashaf Junaid Abdul Rehman |
| author_facet | Saba Kabir Hasan Ejaz Syed Zajif Hussain Muhammad Asif Rasheed Kashaf Junaid Abdul Rehman |
| author_sort | Saba Kabir |
| collection | DOAJ |
| description | In the present study genotypic resistance of bacterial strain to first- and second-line anti-tuberculosis drugs was determined by Line probe assay. Toxic effect of tellurite on the growth of Mycobacterium tuberculosis (MTB) was determined by growing cells in different concentrations of tellurite (1 to 5 mM). Morphological effects of tellurite and uptake of metal in bacterial cells were confirmed by Scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis. Change in fold expression of the efflux pump gene was measured by RT-PCR. Mycobacterial strain was characterized as XDR-TB based on the genotypic resistance to rifampicin and isoniazid, along with resistance to fluoroquinolones and second line injectable. XDR-TB showed black colonies in tellurite presence and growth was delayed (2–3 weeks) when compared with the control. The reduced cell size, metal accumulation and the characteristic tellurite peaks appeared in metal-treated cells. MTB showed MIC value of 1 mM and had high susceptibility for higher concentrations (2–5 mM). However, no significant metal inhibitory effect on the mmpL7 efflux system was determined. Tellurite shows significant growth reduction potential against XDR-TB strain. However, the exact mechanism of action needs to be elucidated with further research. |
| first_indexed | 2024-04-09T17:20:13Z |
| format | Article |
| id | doaj.art-1c49d0a55c3f41aeb7553b17b86968a6 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-09T17:20:13Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-1c49d0a55c3f41aeb7553b17b86968a62023-04-19T04:21:56ZengElsevierJournal of King Saud University: Science1018-36472023-05-01354102629Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pumpSaba Kabir0Hasan Ejaz1Syed Zajif Hussain2Muhammad Asif Rasheed3Kashaf Junaid4Abdul Rehman5Department of Microbiology, Faculty of Life Sciences, University of the Central Punjab, Lahore PakistanDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf 72388, Saudi ArabiaDepartment of Chemistry, SBA School of Sciences and Engineering, University of Management Sciences, Lahore PakistanCOMSATS University Islamabad, Sahiwal campus, Sahiwal PakistanDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Al Jouf 72388, Saudi ArabiaInstitute of Microbiology and Molecular Genetics, University of the Punjab, Quaid Azam Campus, Lahore 54590, Pakistan; Correspondence author at: Institute of Microbiology & Molecular Genetics University of the Punjab, New Campus, Lahore 54590, Pakistan.In the present study genotypic resistance of bacterial strain to first- and second-line anti-tuberculosis drugs was determined by Line probe assay. Toxic effect of tellurite on the growth of Mycobacterium tuberculosis (MTB) was determined by growing cells in different concentrations of tellurite (1 to 5 mM). Morphological effects of tellurite and uptake of metal in bacterial cells were confirmed by Scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis. Change in fold expression of the efflux pump gene was measured by RT-PCR. Mycobacterial strain was characterized as XDR-TB based on the genotypic resistance to rifampicin and isoniazid, along with resistance to fluoroquinolones and second line injectable. XDR-TB showed black colonies in tellurite presence and growth was delayed (2–3 weeks) when compared with the control. The reduced cell size, metal accumulation and the characteristic tellurite peaks appeared in metal-treated cells. MTB showed MIC value of 1 mM and had high susceptibility for higher concentrations (2–5 mM). However, no significant metal inhibitory effect on the mmpL7 efflux system was determined. Tellurite shows significant growth reduction potential against XDR-TB strain. However, the exact mechanism of action needs to be elucidated with further research.http://www.sciencedirect.com/science/article/pii/S1018364723000915TelluriteXDR-TBmmpL7 geneDrug efflux pump |
| spellingShingle | Saba Kabir Hasan Ejaz Syed Zajif Hussain Muhammad Asif Rasheed Kashaf Junaid Abdul Rehman Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump Journal of King Saud University: Science Tellurite XDR-TB mmpL7 gene Drug efflux pump |
| title | Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| title_full | Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| title_fullStr | Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| title_full_unstemmed | Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| title_short | Effect of tellurite on growth of extensively drug resistant (XDR) Mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| title_sort | effect of tellurite on growth of extensively drug resistant xdr mycobacterium tuberculosis and action on mycobacterial drug efflux pump |
| topic | Tellurite XDR-TB mmpL7 gene Drug efflux pump |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723000915 |
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