Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation
Objective: To analyze the changes in phenolic composition and antioxidant function of mulberry beverage before and after fermentation. Methods: Mulberry beverage was prepared by a secondary fermentation method using Pichia kudriavzevii and Weissella confusa. The scavenge rate and reducing power of p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2024-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040005 |
| _version_ | 1797357896779956224 |
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| author | Xiaoyun HAN Yuting TAO Jiaying ZHAN Junhong LI Baochang DU Jing CHEN Qingshen SUN |
| author_facet | Xiaoyun HAN Yuting TAO Jiaying ZHAN Junhong LI Baochang DU Jing CHEN Qingshen SUN |
| author_sort | Xiaoyun HAN |
| collection | DOAJ |
| description | Objective: To analyze the changes in phenolic composition and antioxidant function of mulberry beverage before and after fermentation. Methods: Mulberry beverage was prepared by a secondary fermentation method using Pichia kudriavzevii and Weissella confusa. The scavenge rate and reducing power of phenolic substances on DPPH, OH, ABTS+ free radicals before and after mulberry fermentation were compared. The differential metabolites in different samples were identified using non targeted metabolomics techniques. Then the correlation between changes in phenolic composition before and after fermentation and antioxidant performance was further explored. Results: The free radical scavenging rates of mulberry phenolic substances (1 mg/mL) on DPPH increased from 45.97%±2.23% before fermentation to 59.01%±3.59% after fermentation (P<0.05), while the scavenging rates on OH increased from 68.68%±1.23% to 78.55%±0.57% after fermentation (P<0.05). The reducing power of phenolic substances in fermented mulberry beverages was significantly increased (P<0.05). There was no significant difference in the ability of phenolic compounds to scavenge ABTS+ radicals before and after fermentation (P>0.05). A total of 46 differential metabolites were screened from mulberry phenolic substances before and after fermentation, including 3 anthocyanins, 10 phenolic acids, 18 flavonoids, and 15 other phenolic substances, of which 26 metabolites were up-regulated and another 20 metabolites were down-regulated, involving a total of 4 related metabolic pathways, including the biosynthesis of secondary metabolites in plants, phenylpropanoid biosynthesis, flavonoid biosynthesis, and anthocyanin biosynthesis. 58.70% of phenolic substances were positively correlated with DPPH and OH radical clearance rates. Conclusion: Both the changes in phenolic composition and increased content of mulberry after fermentation result in a significant upregulation of antioxidant capacity. |
| first_indexed | 2024-03-08T14:52:07Z |
| format | Article |
| id | doaj.art-1c4a4c716e4c42b4adca2b180b6484b8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-08T14:52:07Z |
| publishDate | 2024-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-1c4a4c716e4c42b4adca2b180b6484b82024-01-11T01:27:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-01-0145228028810.13386/j.issn1002-0306.20230400052023040005-2Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after FermentationXiaoyun HAN0Yuting TAO1Jiaying ZHAN2Junhong LI3Baochang DU4Jing CHEN5Qingshen SUN6School of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, ChinaSchool of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, ChinaSchool of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, ChinaSchool of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, ChinaGannan Forest Farm, Qiqihar 162100, ChinaHebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering,Qinhuangdao 066102, ChinaSchool of Life Sciences, Heilongjiang University, Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080, ChinaObjective: To analyze the changes in phenolic composition and antioxidant function of mulberry beverage before and after fermentation. Methods: Mulberry beverage was prepared by a secondary fermentation method using Pichia kudriavzevii and Weissella confusa. The scavenge rate and reducing power of phenolic substances on DPPH, OH, ABTS+ free radicals before and after mulberry fermentation were compared. The differential metabolites in different samples were identified using non targeted metabolomics techniques. Then the correlation between changes in phenolic composition before and after fermentation and antioxidant performance was further explored. Results: The free radical scavenging rates of mulberry phenolic substances (1 mg/mL) on DPPH increased from 45.97%±2.23% before fermentation to 59.01%±3.59% after fermentation (P<0.05), while the scavenging rates on OH increased from 68.68%±1.23% to 78.55%±0.57% after fermentation (P<0.05). The reducing power of phenolic substances in fermented mulberry beverages was significantly increased (P<0.05). There was no significant difference in the ability of phenolic compounds to scavenge ABTS+ radicals before and after fermentation (P>0.05). A total of 46 differential metabolites were screened from mulberry phenolic substances before and after fermentation, including 3 anthocyanins, 10 phenolic acids, 18 flavonoids, and 15 other phenolic substances, of which 26 metabolites were up-regulated and another 20 metabolites were down-regulated, involving a total of 4 related metabolic pathways, including the biosynthesis of secondary metabolites in plants, phenylpropanoid biosynthesis, flavonoid biosynthesis, and anthocyanin biosynthesis. 58.70% of phenolic substances were positively correlated with DPPH and OH radical clearance rates. Conclusion: Both the changes in phenolic composition and increased content of mulberry after fermentation result in a significant upregulation of antioxidant capacity.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040005mulberryfermentationpolyphenolsantioxidant activitynon-targeted metabolomics |
| spellingShingle | Xiaoyun HAN Yuting TAO Jiaying ZHAN Junhong LI Baochang DU Jing CHEN Qingshen SUN Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation Shipin gongye ke-ji mulberry fermentation polyphenols antioxidant activity non-targeted metabolomics |
| title | Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation |
| title_full | Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation |
| title_fullStr | Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation |
| title_full_unstemmed | Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation |
| title_short | Analysis of Phenolic Composition Changes and Antioxidant Activity of Mulberry before and after Fermentation |
| title_sort | analysis of phenolic composition changes and antioxidant activity of mulberry before and after fermentation |
| topic | mulberry fermentation polyphenols antioxidant activity non-targeted metabolomics |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040005 |
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