A soybean GmDREB3 gene contributes to drought tolerance in wheat
Abstract DREB transcription factor can significantly improve the plant resistance to drought, high salt, low temperature, high temperature, and other abiotic stress. A previous study showed that DREB transcription factor gene GmDREB3 from soybean can improve drought resistance in tobacco, however, w...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-11-01
|
| Series: | Food and Energy Security |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fes3.426 |
| _version_ | 1798019057940692992 |
|---|---|
| author | Xingxuan Bai Yongbin Zhou Md Ashraful Islam Weibin Zhang Lei Ning Bingqi Ling Yanxia Wang Zhao‐Shi Xu Daizhen Sun Ming Chen |
| author_facet | Xingxuan Bai Yongbin Zhou Md Ashraful Islam Weibin Zhang Lei Ning Bingqi Ling Yanxia Wang Zhao‐Shi Xu Daizhen Sun Ming Chen |
| author_sort | Xingxuan Bai |
| collection | DOAJ |
| description | Abstract DREB transcription factor can significantly improve the plant resistance to drought, high salt, low temperature, high temperature, and other abiotic stress. A previous study showed that DREB transcription factor gene GmDREB3 from soybean can improve drought resistance in tobacco, however, whether it can improve drought resistance in wheat needs to be further investigated. In the present study, GmDREB3 was transferred to a wheat variety, Shi366 and two positive lines of the T5 generation were obtained by additive culture and molecular identification. GmDREB3‐OE (overexpression) lines conferred drought tolerance characteristics, including higher survival rates, lower malondialdehyde (MDA) content, lower relative conductivity, higher chlorophyll content, and higher proline content than Shi366 wheat plants under drought stress. The SPAD, Fv/Fm, panicle number, and grain yield of GmDREB3‐OE lines were increased but the transpiration rate was decreased, compared to the Shi366 plants in the field condition under drought stress. Significant differences in the stress indices were observed between the GmDREB3–OE lines and Shi366 wheat plants, which suggested that the GmDREB3 significantly improved wheat tolerance to drought stress. In addition, RT‐qPCR analysis indicated that GmDREB3 could enhance the expression of drought‐related genes in wheat. Therefore, GmDREB3 significantly increased drought tolerance in wheat plants along with improved physiological and agronomic traits and grain yield. |
| first_indexed | 2024-04-11T16:33:22Z |
| format | Article |
| id | doaj.art-1c4b89285713483fb6871e5aa4177419 |
| institution | Directory Open Access Journal |
| issn | 2048-3694 |
| language | English |
| last_indexed | 2024-04-11T16:33:22Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food and Energy Security |
| spelling | doaj.art-1c4b89285713483fb6871e5aa41774192022-12-22T04:13:54ZengWileyFood and Energy Security2048-36942022-11-01114n/an/a10.1002/fes3.426A soybean GmDREB3 gene contributes to drought tolerance in wheatXingxuan Bai0Yongbin Zhou1Md Ashraful Islam2Weibin Zhang3Lei Ning4Bingqi Ling5Yanxia Wang6Zhao‐Shi Xu7Daizhen Sun8Ming Chen9College of Agriculture Shanxi Agricultural University Taigu Shanxi ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing ChinaCollege of Agriculture Shanxi Agricultural University Taigu Shanxi ChinaMaize Research Institute Shanxi Agricultural University Xinzhou ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing ChinaShijiazhuang Academy of Agricultural and Forestry Sciences Research Center of Wheat Engineering Technology of Hebei Shijiazhuang Hebei ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing ChinaCollege of Agriculture Shanxi Agricultural University Taigu Shanxi ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing ChinaAbstract DREB transcription factor can significantly improve the plant resistance to drought, high salt, low temperature, high temperature, and other abiotic stress. A previous study showed that DREB transcription factor gene GmDREB3 from soybean can improve drought resistance in tobacco, however, whether it can improve drought resistance in wheat needs to be further investigated. In the present study, GmDREB3 was transferred to a wheat variety, Shi366 and two positive lines of the T5 generation were obtained by additive culture and molecular identification. GmDREB3‐OE (overexpression) lines conferred drought tolerance characteristics, including higher survival rates, lower malondialdehyde (MDA) content, lower relative conductivity, higher chlorophyll content, and higher proline content than Shi366 wheat plants under drought stress. The SPAD, Fv/Fm, panicle number, and grain yield of GmDREB3‐OE lines were increased but the transpiration rate was decreased, compared to the Shi366 plants in the field condition under drought stress. Significant differences in the stress indices were observed between the GmDREB3–OE lines and Shi366 wheat plants, which suggested that the GmDREB3 significantly improved wheat tolerance to drought stress. In addition, RT‐qPCR analysis indicated that GmDREB3 could enhance the expression of drought‐related genes in wheat. Therefore, GmDREB3 significantly increased drought tolerance in wheat plants along with improved physiological and agronomic traits and grain yield.https://doi.org/10.1002/fes3.426agronomic traitsdrought tolerancephysiological traitstransgenic wheat |
| spellingShingle | Xingxuan Bai Yongbin Zhou Md Ashraful Islam Weibin Zhang Lei Ning Bingqi Ling Yanxia Wang Zhao‐Shi Xu Daizhen Sun Ming Chen A soybean GmDREB3 gene contributes to drought tolerance in wheat Food and Energy Security agronomic traits drought tolerance physiological traits transgenic wheat |
| title | A soybean GmDREB3 gene contributes to drought tolerance in wheat |
| title_full | A soybean GmDREB3 gene contributes to drought tolerance in wheat |
| title_fullStr | A soybean GmDREB3 gene contributes to drought tolerance in wheat |
| title_full_unstemmed | A soybean GmDREB3 gene contributes to drought tolerance in wheat |
| title_short | A soybean GmDREB3 gene contributes to drought tolerance in wheat |
| title_sort | soybean gmdreb3 gene contributes to drought tolerance in wheat |
| topic | agronomic traits drought tolerance physiological traits transgenic wheat |
| url | https://doi.org/10.1002/fes3.426 |
| work_keys_str_mv | AT xingxuanbai asoybeangmdreb3genecontributestodroughttoleranceinwheat AT yongbinzhou asoybeangmdreb3genecontributestodroughttoleranceinwheat AT mdashrafulislam asoybeangmdreb3genecontributestodroughttoleranceinwheat AT weibinzhang asoybeangmdreb3genecontributestodroughttoleranceinwheat AT leining asoybeangmdreb3genecontributestodroughttoleranceinwheat AT bingqiling asoybeangmdreb3genecontributestodroughttoleranceinwheat AT yanxiawang asoybeangmdreb3genecontributestodroughttoleranceinwheat AT zhaoshixu asoybeangmdreb3genecontributestodroughttoleranceinwheat AT daizhensun asoybeangmdreb3genecontributestodroughttoleranceinwheat AT mingchen asoybeangmdreb3genecontributestodroughttoleranceinwheat AT xingxuanbai soybeangmdreb3genecontributestodroughttoleranceinwheat AT yongbinzhou soybeangmdreb3genecontributestodroughttoleranceinwheat AT mdashrafulislam soybeangmdreb3genecontributestodroughttoleranceinwheat AT weibinzhang soybeangmdreb3genecontributestodroughttoleranceinwheat AT leining soybeangmdreb3genecontributestodroughttoleranceinwheat AT bingqiling soybeangmdreb3genecontributestodroughttoleranceinwheat AT yanxiawang soybeangmdreb3genecontributestodroughttoleranceinwheat AT zhaoshixu soybeangmdreb3genecontributestodroughttoleranceinwheat AT daizhensun soybeangmdreb3genecontributestodroughttoleranceinwheat AT mingchen soybeangmdreb3genecontributestodroughttoleranceinwheat |