Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium
Bagasse contain high cellulose which potentially to be used to raw material for producing cellulase enzyme using fungi Phanerochaete chrysosporium. This fungus has ability to produce cellulase enzymes from substrates which contain cellulose and also produce enzymes that can degrade lignin content so...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Politeknik Negeri Malang
2017-10-01
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| Series: | Jurnal Teknik Kimia dan Lingkungan |
| Subjects: | |
| Online Access: | http://jurnal.polinema.ac.id/index.php/jtkl/article/view/1708 |
| _version_ | 1797831033484214272 |
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| author | Sri Rulianah Zakijah Irfin Mufid Prayitno |
| author_facet | Sri Rulianah Zakijah Irfin Mufid Prayitno |
| author_sort | Sri Rulianah |
| collection | DOAJ |
| description | Bagasse contain high cellulose which potentially to be used to raw material for producing cellulase enzyme using fungi Phanerochaete chrysosporium. This fungus has ability to produce cellulase enzymes from substrates which contain cellulose and also produce enzymes that can degrade lignin content so it didn’t need the delignification process. The objective of this study was to convert cellulose in bagasse to be crude cellulase enzymes by using Phanerochaete chrysosporium and determine the effect of substrate concentration and fermentation time to the enzyme activity. This research was conducted by drying and reducing the bagasse particle size, rejuvenating mold Phanerocheate chrysoporium, making inoculum in liquid medium, fermenting bagasse in accordance with the variable, with media NLM (nitrogen limited media) using Phanerocheate chrysoporium. Fermentation results were filtered, and it was analyzed the activity of crude cellulase. The variable in this study was the time of fermentation 9, 11, 13, 15, and 17 days and substrate concentration: 5, 6, and 7 % b/v. Crude cellulose was filtered and was analyzed the enzyme activity by DNS (dinitro salicylic acid) reagent, using UV-Vis spectrophotometer. The best result of this study was the crude cellulase with highest activity 91,304 U/mL for 7 % substrate concentration with fermentation time 17 days. |
| first_indexed | 2024-04-09T13:46:37Z |
| format | Article |
| id | doaj.art-1c4e010497164e6c8be8fdf7518af470 |
| institution | Directory Open Access Journal |
| issn | 2579-8537 2579-9746 |
| language | English |
| last_indexed | 2024-04-09T13:46:37Z |
| publishDate | 2017-10-01 |
| publisher | Politeknik Negeri Malang |
| record_format | Article |
| series | Jurnal Teknik Kimia dan Lingkungan |
| spelling | doaj.art-1c4e010497164e6c8be8fdf7518af4702023-05-09T03:24:30ZengPoliteknik Negeri MalangJurnal Teknik Kimia dan Lingkungan2579-85372579-97462017-10-0111172710.33795/jtkl.v1i1.241713Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporiumSri Rulianah0Zakijah Irfin1Mufid2Prayitno3Jurusan Teknik Kimia, Politeknik Negeri Malang, Jl. Soekarno-Hatta No.9, Malang, IndonesiaJurusan Teknik Kimia, Politeknik Negeri Malang, Jl. Soekarno-Hatta No.9, Malang, IndonesiaJurusan Teknik Kimia, Politeknik Negeri Malang, Jl. Soekarno-Hatta No.9, Malang, IndonesiaJurusan Teknik Kimia, Politeknik Negeri Malang, Jl. Soekarno-Hatta No.9, Malang, IndonesiaBagasse contain high cellulose which potentially to be used to raw material for producing cellulase enzyme using fungi Phanerochaete chrysosporium. This fungus has ability to produce cellulase enzymes from substrates which contain cellulose and also produce enzymes that can degrade lignin content so it didn’t need the delignification process. The objective of this study was to convert cellulose in bagasse to be crude cellulase enzymes by using Phanerochaete chrysosporium and determine the effect of substrate concentration and fermentation time to the enzyme activity. This research was conducted by drying and reducing the bagasse particle size, rejuvenating mold Phanerocheate chrysoporium, making inoculum in liquid medium, fermenting bagasse in accordance with the variable, with media NLM (nitrogen limited media) using Phanerocheate chrysoporium. Fermentation results were filtered, and it was analyzed the activity of crude cellulase. The variable in this study was the time of fermentation 9, 11, 13, 15, and 17 days and substrate concentration: 5, 6, and 7 % b/v. Crude cellulose was filtered and was analyzed the enzyme activity by DNS (dinitro salicylic acid) reagent, using UV-Vis spectrophotometer. The best result of this study was the crude cellulase with highest activity 91,304 U/mL for 7 % substrate concentration with fermentation time 17 days.http://jurnal.polinema.ac.id/index.php/jtkl/article/view/1708bagassecrude cellulasefermentationphanerochaete chrysosporium. |
| spellingShingle | Sri Rulianah Zakijah Irfin Mufid Prayitno Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium Jurnal Teknik Kimia dan Lingkungan bagasse crude cellulase fermentation phanerochaete chrysosporium. |
| title | Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium |
| title_full | Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium |
| title_fullStr | Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium |
| title_full_unstemmed | Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium |
| title_short | Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium |
| title_sort | produksi crude selulase dari bahan baku ampas tebu menggunakan kapang phanerochaete chrysosporium |
| topic | bagasse crude cellulase fermentation phanerochaete chrysosporium. |
| url | http://jurnal.polinema.ac.id/index.php/jtkl/article/view/1708 |
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