Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants
In this study, we characterized the physical form of allergenic Cry j 1 in the urban atmosphere. Through an immunofluorescence antibody method, we showed that allergenic Cry j 1 exists as fine particles (≤1.1 μm). To determine Cry j 1 concentrations and its particle size distribution, we used the EL...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2012-03-01
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| Series: | Asian Journal of Atmospheric Environment |
| Subjects: | |
| Online Access: | http://asianjae.org/_common/do.php?a=full&b=11&bidx=1573&aidx=19856 |
| _version_ | 1797719836722200576 |
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| author | Qingyue Wang Jun Morita Xiumin Gong Shinichi Nakamura Miho Suzuki Senlin Lu Kazuhiko Sekiguchi Takuya Nakajima Daisuke Nakajima Makoto Miwa |
| author_facet | Qingyue Wang Jun Morita Xiumin Gong Shinichi Nakamura Miho Suzuki Senlin Lu Kazuhiko Sekiguchi Takuya Nakajima Daisuke Nakajima Makoto Miwa |
| author_sort | Qingyue Wang |
| collection | DOAJ |
| description | In this study, we characterized the physical form of allergenic Cry j 1 in the urban atmosphere. Through an immunofluorescence antibody method, we showed that allergenic Cry j 1 exists as fine particles (≤1.1 μm). To determine Cry j 1 concentrations and its particle size distribution, we used the ELISA method to confirm that most Cry j 1 exists as fine particles in the urban atmosphere and is found at high concentrations on fine day next to rainy day. Furthermore, we evaluated Cry j 1 denaturation by using the Biacore J system based on the surface plasmon resonence (SPR) principle and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We showed that the dissociation constant (KD) of Cry j 1 that has been exposed to urban polluted air is lower (1.76 ×10-14 M) than that of Cry j 1 (1.32×10-9-3.37×10-9 M) of original pollen grains that has not been exposed to air pollutants. Cry j 1 turns into low molecular weight proteins by reacting with various acidic solutions. In sum, we showed that allergenic Cry j 1 exists as fine particles that can deposit in the lower respiratory tract. This finding clarifies the relationship between Japanese cedar pollinosis and air pollutants. |
| first_indexed | 2024-03-12T09:10:35Z |
| format | Article |
| id | doaj.art-1c4fa86325ce45efa3b8edd19f4d8879 |
| institution | Directory Open Access Journal |
| issn | 1976-6912 2287-1160 |
| language | English |
| last_indexed | 2024-03-12T09:10:35Z |
| publishDate | 2012-03-01 |
| publisher | Springer |
| record_format | Article |
| series | Asian Journal of Atmospheric Environment |
| spelling | doaj.art-1c4fa86325ce45efa3b8edd19f4d88792023-09-02T15:00:19ZengSpringerAsian Journal of Atmospheric Environment1976-69122287-11602012-03-0161334010.5572/ajae.2012.6.1.033Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air PollutantsQingyue Wang0Jun Morita 1Xiumin Gong 2Shinichi Nakamura 3Miho Suzuki 4Senlin Lu5Kazuhiko Sekiguchi6Takuya Nakajima7Daisuke Nakajima8Makoto Miwa9Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, ChinaGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanGraduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JapanResarch Center for Environmental Risk, National institute for Environmental Studies, 16-2 Onogawa, Tsukuba City, Ibaraki 305-8506, JapanCenter for Environmental Science in Saitama, 914 Kamitanadare, Kisaimachi, Kitasaitama-gun, Saitama 347-0115, JapanIn this study, we characterized the physical form of allergenic Cry j 1 in the urban atmosphere. Through an immunofluorescence antibody method, we showed that allergenic Cry j 1 exists as fine particles (≤1.1 μm). To determine Cry j 1 concentrations and its particle size distribution, we used the ELISA method to confirm that most Cry j 1 exists as fine particles in the urban atmosphere and is found at high concentrations on fine day next to rainy day. Furthermore, we evaluated Cry j 1 denaturation by using the Biacore J system based on the surface plasmon resonence (SPR) principle and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We showed that the dissociation constant (KD) of Cry j 1 that has been exposed to urban polluted air is lower (1.76 ×10-14 M) than that of Cry j 1 (1.32×10-9-3.37×10-9 M) of original pollen grains that has not been exposed to air pollutants. Cry j 1 turns into low molecular weight proteins by reacting with various acidic solutions. In sum, we showed that allergenic Cry j 1 exists as fine particles that can deposit in the lower respiratory tract. This finding clarifies the relationship between Japanese cedar pollinosis and air pollutants.http://asianjae.org/_common/do.php?a=full&b=11&bidx=1573&aidx=19856japanese cedar pollinosisallergenic cry j 1surface plasmon resonance (spr)sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page)air pollutants |
| spellingShingle | Qingyue Wang Jun Morita Xiumin Gong Shinichi Nakamura Miho Suzuki Senlin Lu Kazuhiko Sekiguchi Takuya Nakajima Daisuke Nakajima Makoto Miwa Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants Asian Journal of Atmospheric Environment japanese cedar pollinosis allergenic cry j 1 surface plasmon resonance (spr) sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) air pollutants |
| title | Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants |
| title_full | Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants |
| title_fullStr | Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants |
| title_full_unstemmed | Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants |
| title_short | Characterization of the Physical Form of Allergenic Cry j 1 in the Urban Atmosphere and Determination of Cry j 1 Denaturation by Air Pollutants |
| title_sort | characterization of the physical form of allergenic cry j 1 in the urban atmosphere and determination of cry j 1 denaturation by air pollutants |
| topic | japanese cedar pollinosis allergenic cry j 1 surface plasmon resonance (spr) sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) air pollutants |
| url | http://asianjae.org/_common/do.php?a=full&b=11&bidx=1573&aidx=19856 |
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