Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
Abstract Background As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hypert...
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| Format: | Article |
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BMC
2019-12-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-019-6377-7 |
| _version_ | 1818601754929922048 |
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| author | Haojie Zhang Baoyu Hu Jiali Xiong Ting Chen Qianyun Xi Junyi Luo Qingyan Jiang Jiajie Sun Yongliang Zhang |
| author_facet | Haojie Zhang Baoyu Hu Jiali Xiong Ting Chen Qianyun Xi Junyi Luo Qingyan Jiang Jiajie Sun Yongliang Zhang |
| author_sort | Haojie Zhang |
| collection | DOAJ |
| description | Abstract Background As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hyperthermia in summer. However, the expression and functions of circRNAs in the pituitary of sows and whether they participate in heat stress adaption are still unclear. Results In this study, we found that high temperature over the thermoneutral zone of sows during the summer increased the serum heat shock protein 70 (HSP70) level, decreased the superoxide dismutase (SOD) vitality and prolactin (PRL) concentration, and induced heat stress in sows. Then, we explored circRNA in the pituitary of heat-stressed and normal sows using RNA sequencing and bioinformatics analysis. In total, 12,035 circRNAs were detected, with 59 circRNAs differentially expressed, including 42 up-regulated and 17 down-regulated circRNAs in pituitaries of the heat-stressed sows. Six randomly selected circRNAs were identified through reverse transcription PCR followed by DNA sequencing and other 7 randomly selected differentially expressed circRNAs were verified by quantitative real-time PCR analysis. The predicted target genes regulated by circRNAs through sponging microRNAs (miRNAs) were enriched in metabolic pathway. Furthermore, the predicted circRNA–miRNA–mRNA interactions showed that some circRNAs might sponge miRNAs to regulate pituitary-specific genes and heat shock protein family members, indicating circRNA’s roles in pituitary hormone secretion and heat stress response. Conclusions Our results provided a meaningful reference to understand the functions of circRNA in the porcine pituitary and the mechanisms by which circRNA may participate in animals’ response to heat stress. |
| first_indexed | 2024-12-16T12:56:25Z |
| format | Article |
| id | doaj.art-1c53007ab89b4694af40e6e637fdb902 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-16T12:56:25Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-1c53007ab89b4694af40e6e637fdb9022022-12-21T22:31:00ZengBMCBMC Genomics1471-21642019-12-0120111310.1186/s12864-019-6377-7Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sowsHaojie Zhang0Baoyu Hu1Jiali Xiong2Ting Chen3Qianyun Xi4Junyi Luo5Qingyan Jiang6Jiajie Sun7Yongliang Zhang8Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityGuangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityAbstract Background As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hyperthermia in summer. However, the expression and functions of circRNAs in the pituitary of sows and whether they participate in heat stress adaption are still unclear. Results In this study, we found that high temperature over the thermoneutral zone of sows during the summer increased the serum heat shock protein 70 (HSP70) level, decreased the superoxide dismutase (SOD) vitality and prolactin (PRL) concentration, and induced heat stress in sows. Then, we explored circRNA in the pituitary of heat-stressed and normal sows using RNA sequencing and bioinformatics analysis. In total, 12,035 circRNAs were detected, with 59 circRNAs differentially expressed, including 42 up-regulated and 17 down-regulated circRNAs in pituitaries of the heat-stressed sows. Six randomly selected circRNAs were identified through reverse transcription PCR followed by DNA sequencing and other 7 randomly selected differentially expressed circRNAs were verified by quantitative real-time PCR analysis. The predicted target genes regulated by circRNAs through sponging microRNAs (miRNAs) were enriched in metabolic pathway. Furthermore, the predicted circRNA–miRNA–mRNA interactions showed that some circRNAs might sponge miRNAs to regulate pituitary-specific genes and heat shock protein family members, indicating circRNA’s roles in pituitary hormone secretion and heat stress response. Conclusions Our results provided a meaningful reference to understand the functions of circRNA in the porcine pituitary and the mechanisms by which circRNA may participate in animals’ response to heat stress.https://doi.org/10.1186/s12864-019-6377-7HyperthermiaNoncoding RNAPituitaryProfilingSows |
| spellingShingle | Haojie Zhang Baoyu Hu Jiali Xiong Ting Chen Qianyun Xi Junyi Luo Qingyan Jiang Jiajie Sun Yongliang Zhang Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows BMC Genomics Hyperthermia Noncoding RNA Pituitary Profiling Sows |
| title | Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows |
| title_full | Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows |
| title_fullStr | Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows |
| title_full_unstemmed | Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows |
| title_short | Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows |
| title_sort | genomewide analysis of circular rna in pituitaries of normal and heat stressed sows |
| topic | Hyperthermia Noncoding RNA Pituitary Profiling Sows |
| url | https://doi.org/10.1186/s12864-019-6377-7 |
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