Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells
Objective: The major pathophysiological features of β-thalassemia are anemia and ineffective erythropoiesis. Ineffective erythropoiesis has been shown by an intense marrow erythroid hyperplasia and increased apoptosis during basophilic to orthochromatic normoblast stages. Some cytokines like interfe...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2013-04-01
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| Series: | Asian Journal of Medical Sciences |
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| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/6805 |
| _version_ | 1818909321584443392 |
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| author | Umesh Prasad Gupta |
| author_facet | Umesh Prasad Gupta |
| author_sort | Umesh Prasad Gupta |
| collection | DOAJ |
| description | Objective: The major pathophysiological features of β-thalassemia are anemia and ineffective erythropoiesis. Ineffective erythropoiesis has been shown by an intense marrow erythroid hyperplasia and increased apoptosis during basophilic to orthochromatic normoblast stages. Some cytokines like interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin-1 (IL-1) have been found to involved in apoptosis. Although the pro-apoptotic activity of IFN-γ and TNF-α is well documented, there are only few studies on IL-1, especially on erythroid lineage. In this in vitro study, the role of cytokine IL-1α and IL-1β in apoptosis of erythroid progenitor cells from β- thalassemia/HbE patients was assessed.
Methods: Erythroid progenitor cells were isolated from peripheral blood of healthy subjects and β-thalassemia/HbE patients. Cells were then cultured, with and without 20 ng/ml IL-1α and IL-1β. Total cells and percent cell viability were performed by using trypan blue staining. Percent cell apoptosis was analyzed by using flow cytometer.
Results: Both IL-1α and IL-1β were significantly decreased erythroid progenitor cells. IL-1 at 20 ng/ml reduced the glycophorin A positive cells and percent cell viability of erythroid progenitor cells from β-thalassemia/HbE patients, while there was increased apoptosis in this group. The highest percent apoptosis was observed in 20 ng/ml IL-1β treated β-thalassemia/HbE erythroid progenitor cells.
Conclusion: IL-1β could be involved in apoptosis of erythroid progenitor cells from β-thalassemia/HbE patients which might be related with ineffective erythropoiesis of the disease. |
| first_indexed | 2024-12-19T22:25:03Z |
| format | Article |
| id | doaj.art-1c55f168013a47c297d3fa8c74f2f358 |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-12-19T22:25:03Z |
| publishDate | 2013-04-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-1c55f168013a47c297d3fa8c74f2f3582022-12-21T20:03:31ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762013-04-0134814https://doi.org/10.3126/ajms.v3i4.6805Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor CellsUmesh Prasad Gupta 0Department of Medical Laboratory Science, Faculty of Science and Technology, Pokhara UniversityObjective: The major pathophysiological features of β-thalassemia are anemia and ineffective erythropoiesis. Ineffective erythropoiesis has been shown by an intense marrow erythroid hyperplasia and increased apoptosis during basophilic to orthochromatic normoblast stages. Some cytokines like interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin-1 (IL-1) have been found to involved in apoptosis. Although the pro-apoptotic activity of IFN-γ and TNF-α is well documented, there are only few studies on IL-1, especially on erythroid lineage. In this in vitro study, the role of cytokine IL-1α and IL-1β in apoptosis of erythroid progenitor cells from β- thalassemia/HbE patients was assessed. Methods: Erythroid progenitor cells were isolated from peripheral blood of healthy subjects and β-thalassemia/HbE patients. Cells were then cultured, with and without 20 ng/ml IL-1α and IL-1β. Total cells and percent cell viability were performed by using trypan blue staining. Percent cell apoptosis was analyzed by using flow cytometer. Results: Both IL-1α and IL-1β were significantly decreased erythroid progenitor cells. IL-1 at 20 ng/ml reduced the glycophorin A positive cells and percent cell viability of erythroid progenitor cells from β-thalassemia/HbE patients, while there was increased apoptosis in this group. The highest percent apoptosis was observed in 20 ng/ml IL-1β treated β-thalassemia/HbE erythroid progenitor cells. Conclusion: IL-1β could be involved in apoptosis of erythroid progenitor cells from β-thalassemia/HbE patients which might be related with ineffective erythropoiesis of the disease.https://www.nepjol.info/index.php/AJMS/article/view/6805ineffective erythropoiesisapoptosisil-1beta-thalassemiahbe |
| spellingShingle | Umesh Prasad Gupta Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells Asian Journal of Medical Sciences ineffective erythropoiesis apoptosis il-1 beta-thalassemia hbe |
| title | Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells |
| title_full | Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells |
| title_fullStr | Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells |
| title_full_unstemmed | Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells |
| title_short | Interleukin-1 involved in Apoptosis of Beta-Thalassemia/Hemoglobin E Erythroid Progenitor Cells |
| title_sort | interleukin 1 involved in apoptosis of beta thalassemia hemoglobin e erythroid progenitor cells |
| topic | ineffective erythropoiesis apoptosis il-1 beta-thalassemia hbe |
| url | https://www.nepjol.info/index.php/AJMS/article/view/6805 |
| work_keys_str_mv | AT umeshprasadgupta interleukin1involvedinapoptosisofbetathalassemiahemoglobineerythroidprogenitorcells |