Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids
Abstract Background Trypanosoma cruzi is a protozoan parasite characterized by extensive genetic heterogeneity. There are currently six recognised, genetically distinct, monophyletic clades designated discrete typing units (DTUs). TcI has the broadest geographical range and most genetic diversity ev...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-018-2696-9 |
| _version_ | 1818972179884146688 |
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| author | Fabiola Roman Alena M. Iñiguez Matthew Yeo Ana M. Jansen |
| author_facet | Fabiola Roman Alena M. Iñiguez Matthew Yeo Ana M. Jansen |
| author_sort | Fabiola Roman |
| collection | DOAJ |
| description | Abstract Background Trypanosoma cruzi is a protozoan parasite characterized by extensive genetic heterogeneity. There are currently six recognised, genetically distinct, monophyletic clades designated discrete typing units (DTUs). TcI has the broadest geographical range and most genetic diversity evidenced by a wide range of genetic markers applied to isolates spanning a vast geographical range across Latin America. However, little is known of the diversity of TcI that exists within sylvatic mammals across the geographical expanse of Brazil. Results Twenty-nine sylvatic TcI isolates spanning multiple ecologically diverse biomes across Brazil were analyzed by the application of multilocus sequence typing (MLST) using four nuclear housekeeping genes. Results revealed extensive genetic diversity and also incongruence among individual gene trees. There was no association of intralineage genotype with geography or with any particular biome, with the exception of isolates from Caatinga that formed a single cluster. However, haplotypic analyses of METIII and LYT1 constitutive markers provided evidence of recombination events in two isolates derived from Didelphis marsupialis and D. albiventris, respectively. For diversity studies all possible combinations of markers were assessed with the objective of selecting the combination of gene targets that are most resolutive using the minimum number of genes. A panel of just three gene fragments (DHFR-TS, LYT1 and METIII) discriminated 26 out of 35 genotypes. Conclusions These findings showed geographical association of genotypes clustering in Caatinga but more characteristically TcI genotypes widely distributed without specific association to geographical areas or biomes. Importantly, we detected the signature of recombination events at the nuclear level evidenced by haplotypic analysis and incongruence. |
| first_indexed | 2024-12-20T15:04:10Z |
| format | Article |
| id | doaj.art-1c5b061697cb4f6887be024c83189342 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-20T15:04:10Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-1c5b061697cb4f6887be024c831893422022-12-21T19:36:34ZengBMCParasites & Vectors1756-33052018-02-0111111310.1186/s13071-018-2696-9Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphidsFabiola Roman0Alena M. Iñiguez1Matthew Yeo2Ana M. Jansen3Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundacao Oswaldo CruzLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundacao Oswaldo CruzDepartment of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineLaboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundacao Oswaldo CruzAbstract Background Trypanosoma cruzi is a protozoan parasite characterized by extensive genetic heterogeneity. There are currently six recognised, genetically distinct, monophyletic clades designated discrete typing units (DTUs). TcI has the broadest geographical range and most genetic diversity evidenced by a wide range of genetic markers applied to isolates spanning a vast geographical range across Latin America. However, little is known of the diversity of TcI that exists within sylvatic mammals across the geographical expanse of Brazil. Results Twenty-nine sylvatic TcI isolates spanning multiple ecologically diverse biomes across Brazil were analyzed by the application of multilocus sequence typing (MLST) using four nuclear housekeeping genes. Results revealed extensive genetic diversity and also incongruence among individual gene trees. There was no association of intralineage genotype with geography or with any particular biome, with the exception of isolates from Caatinga that formed a single cluster. However, haplotypic analyses of METIII and LYT1 constitutive markers provided evidence of recombination events in two isolates derived from Didelphis marsupialis and D. albiventris, respectively. For diversity studies all possible combinations of markers were assessed with the objective of selecting the combination of gene targets that are most resolutive using the minimum number of genes. A panel of just three gene fragments (DHFR-TS, LYT1 and METIII) discriminated 26 out of 35 genotypes. Conclusions These findings showed geographical association of genotypes clustering in Caatinga but more characteristically TcI genotypes widely distributed without specific association to geographical areas or biomes. Importantly, we detected the signature of recombination events at the nuclear level evidenced by haplotypic analysis and incongruence.http://link.springer.com/article/10.1186/s13071-018-2696-9Trypanosoma cruziChagas diseaseMultilocus sequence typingMLSTRecombinationT. cruzi I |
| spellingShingle | Fabiola Roman Alena M. Iñiguez Matthew Yeo Ana M. Jansen Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids Parasites & Vectors Trypanosoma cruzi Chagas disease Multilocus sequence typing MLST Recombination T. cruzi I |
| title | Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids |
| title_full | Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids |
| title_fullStr | Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids |
| title_full_unstemmed | Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids |
| title_short | Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids |
| title_sort | multilocus sequence typing genetic diversity in trypanosoma cruzi i tci isolates from brazilian didelphids |
| topic | Trypanosoma cruzi Chagas disease Multilocus sequence typing MLST Recombination T. cruzi I |
| url | http://link.springer.com/article/10.1186/s13071-018-2696-9 |
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