Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion
Abstract Background Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13018-020-01936-8 |
| _version_ | 1828149670533136384 |
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| author | Christina Holmes Benjamin D. Elder Wataru Ishida Alexander Perdomo-Pantoja John Locke Ethan Cottrill Sheng-Fu L. Lo Timothy F. Witham |
| author_facet | Christina Holmes Benjamin D. Elder Wataru Ishida Alexander Perdomo-Pantoja John Locke Ethan Cottrill Sheng-Fu L. Lo Timothy F. Witham |
| author_sort | Christina Holmes |
| collection | DOAJ |
| description | Abstract Background Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac crest grafts in spinal fusion. The objective of this study was to directly compare the use of autologous iliac crest with syngeneic femoral and iliac allograft bone in the rat model of lumbar spinal fusion. Methods Single-level bilateral posterolateral intertransverse process lumbar spinal fusion surgery was performed on Lewis rats divided into three experimental groups: iliac crest autograft, syngeneic iliac crest allograft, and syngeneic femoral allograft bone. Eight weeks postoperatively, fusion was evaluated via microCT analysis, manual palpation, and histology. In vitro analysis of the colony-forming and osteogenic capacity of bone marrow cells derived from rat femurs and hips was also performed to determine whether there was a correlation with the fusion efficacy of these graft sources. Results Although no differences were observed between groups in CT fusion mass volumes, iliac allografts displayed an increased number of radiographically fused fusion masses and a higher rate of bilateral fusion via manual palpation. Histologically, hip-derived grafts showed better integration with host bone than femur derived ones, likely associated with the higher concentration of osteogenic progenitor cells observed in hip-derived bone marrow. Conclusions This study demonstrates the feasibility of using syngeneic allograft bone in place of autograft bone within inbred rat fusion models and highlights the need for further study of femoral-derived grafts in fusion. |
| first_indexed | 2024-04-11T21:33:40Z |
| format | Article |
| id | doaj.art-1c5ba600d5c74008aa3f14884c63cd0a |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T21:33:40Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-1c5ba600d5c74008aa3f14884c63cd0a2022-12-22T04:01:50ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2020-09-0115111010.1186/s13018-020-01936-8Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusionChristina Holmes0Benjamin D. Elder1Wataru Ishida2Alexander Perdomo-Pantoja3John Locke4Ethan Cottrill5Sheng-Fu L. Lo6Timothy F. Witham7Department of Chemical and Biomedical Engineering, Florida A&M University-Florida State University College of EngineeringDepartment of Neurosurgery, Mayo ClinicDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineDepartment of Neurosurgery, The Johns Hopkins University, School of MedicineAbstract Background Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac crest grafts in spinal fusion. The objective of this study was to directly compare the use of autologous iliac crest with syngeneic femoral and iliac allograft bone in the rat model of lumbar spinal fusion. Methods Single-level bilateral posterolateral intertransverse process lumbar spinal fusion surgery was performed on Lewis rats divided into three experimental groups: iliac crest autograft, syngeneic iliac crest allograft, and syngeneic femoral allograft bone. Eight weeks postoperatively, fusion was evaluated via microCT analysis, manual palpation, and histology. In vitro analysis of the colony-forming and osteogenic capacity of bone marrow cells derived from rat femurs and hips was also performed to determine whether there was a correlation with the fusion efficacy of these graft sources. Results Although no differences were observed between groups in CT fusion mass volumes, iliac allografts displayed an increased number of radiographically fused fusion masses and a higher rate of bilateral fusion via manual palpation. Histologically, hip-derived grafts showed better integration with host bone than femur derived ones, likely associated with the higher concentration of osteogenic progenitor cells observed in hip-derived bone marrow. Conclusions This study demonstrates the feasibility of using syngeneic allograft bone in place of autograft bone within inbred rat fusion models and highlights the need for further study of femoral-derived grafts in fusion.http://link.springer.com/article/10.1186/s13018-020-01936-8Spinal fusionAnimal modelAutograftAllograftBone marrow cells |
| spellingShingle | Christina Holmes Benjamin D. Elder Wataru Ishida Alexander Perdomo-Pantoja John Locke Ethan Cottrill Sheng-Fu L. Lo Timothy F. Witham Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion Journal of Orthopaedic Surgery and Research Spinal fusion Animal model Autograft Allograft Bone marrow cells |
| title | Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| title_full | Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| title_fullStr | Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| title_full_unstemmed | Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| title_short | Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| title_sort | comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion |
| topic | Spinal fusion Animal model Autograft Allograft Bone marrow cells |
| url | http://link.springer.com/article/10.1186/s13018-020-01936-8 |
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