Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis
Schistosomiasis is a parasitic disease that affects about 166 million people around the world. It is estimated that 5%–10% of individuals with schistosomiasis develop severe forms of the disease, which are characterized by pulmonary hypertension, ascites, periportal fibrosis, and other significant c...
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.605235/full |
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| author | Jordana Batista Santana Tarcísio Vila Verde Santana de Almeida Diego Mota Lopes Brady Page Sergio Costa Oliveira Sergio Costa Oliveira Irismá Souza Luís Eduardo Viana Silva Ribeiro Néstor Adrián Guerrero Gutiérrez Edgar M. Carvalho Edgar M. Carvalho Edgar M. Carvalho Luciana Santos Cardoso Luciana Santos Cardoso Luciana Santos Cardoso |
| author_facet | Jordana Batista Santana Tarcísio Vila Verde Santana de Almeida Diego Mota Lopes Brady Page Sergio Costa Oliveira Sergio Costa Oliveira Irismá Souza Luís Eduardo Viana Silva Ribeiro Néstor Adrián Guerrero Gutiérrez Edgar M. Carvalho Edgar M. Carvalho Edgar M. Carvalho Luciana Santos Cardoso Luciana Santos Cardoso Luciana Santos Cardoso |
| author_sort | Jordana Batista Santana |
| collection | DOAJ |
| description | Schistosomiasis is a parasitic disease that affects about 166 million people around the world. It is estimated that 5%–10% of individuals with schistosomiasis develop severe forms of the disease, which are characterized by pulmonary hypertension, ascites, periportal fibrosis, and other significant complications. The chronic phase of the disease is associated with a Th2 type immune response, but evidence also suggests there are roles for Th1 and Th17 in the development of severe disease. The aim of this study was to evaluate the CD4+ T lymphocyte profile of patients with different degrees of periportal fibrosis secondary to schistosomiasis. These individuals had been treated for schistosomiasis, but since they live in a S. mansoni endemic area, they are at risk of reinfection. They were evaluated in relation to the degree of periportal fibrosis and classified into three groups: without fibrosis or with incipient fibrosis (WF/IFNE), n=12, possible periportal fibrosis/periportal fibrosis, n=13, and advanced periportal fibrosis/advanced periportal fibrosis with portal hypertension, n=4. We observed in the group without fibrosis a balance between the low expression of Th2 cytokines and high expression of T reg cells. As has already been described in the literature, we found an increase of the Th2 cytokines IL-4, IL-5, and IL-13 in the group with periportal fibrosis. In addition, this group showed higher expression of IL-17 and IL-10 but lower IL-10/IL-13 ratio than patients in the WF/IFNE group. Cells from individuals who present any level of fibrosis expressed more TGF-β compared to the WF/IFNE group and a positive correlation with left lobe enlargement and portal vein wall thickness. There was a negative correlation between IL-17 and the thickness of the portal vein wall, but more studies are necessary in order to explore the possible protective role of this cytokine. Despite the fibrosis group having presented a higher expression of pro-fibrotic molecules compared to WF/IFNE patients, it seems there is a regulation through IL-10 and T reg cells that is able to maintain the low morbidity of this group. |
| first_indexed | 2024-12-13T12:45:39Z |
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| last_indexed | 2024-12-13T12:45:39Z |
| publishDate | 2021-02-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-1c5cb861bb844f4caafd4e54674d55602022-12-21T23:45:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-02-011210.3389/fimmu.2021.605235605235Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to SchistosomiasisJordana Batista Santana0Tarcísio Vila Verde Santana de Almeida1Diego Mota Lopes2Brady Page3Sergio Costa Oliveira4Sergio Costa Oliveira5Irismá Souza6Luís Eduardo Viana Silva Ribeiro7Néstor Adrián Guerrero Gutiérrez8Edgar M. Carvalho9Edgar M. Carvalho10Edgar M. Carvalho11Luciana Santos Cardoso12Luciana Santos Cardoso13Luciana Santos Cardoso14 Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BrazilMassachusetts General Hospital, Boston, MA, United StatesDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilInstituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT–DT/CNPq), Salvador, BrazilInstituto de Saúde Irismá Souza, Gandu, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BrazilInstituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT–DT/CNPq), Salvador, BrazilLaboratório de Pesquisas Clínicas, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, BrazilInstituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT–DT/CNPq), Salvador, BrazilDepartamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal da Bahia (UFBA), Salvador, BrazilSchistosomiasis is a parasitic disease that affects about 166 million people around the world. It is estimated that 5%–10% of individuals with schistosomiasis develop severe forms of the disease, which are characterized by pulmonary hypertension, ascites, periportal fibrosis, and other significant complications. The chronic phase of the disease is associated with a Th2 type immune response, but evidence also suggests there are roles for Th1 and Th17 in the development of severe disease. The aim of this study was to evaluate the CD4+ T lymphocyte profile of patients with different degrees of periportal fibrosis secondary to schistosomiasis. These individuals had been treated for schistosomiasis, but since they live in a S. mansoni endemic area, they are at risk of reinfection. They were evaluated in relation to the degree of periportal fibrosis and classified into three groups: without fibrosis or with incipient fibrosis (WF/IFNE), n=12, possible periportal fibrosis/periportal fibrosis, n=13, and advanced periportal fibrosis/advanced periportal fibrosis with portal hypertension, n=4. We observed in the group without fibrosis a balance between the low expression of Th2 cytokines and high expression of T reg cells. As has already been described in the literature, we found an increase of the Th2 cytokines IL-4, IL-5, and IL-13 in the group with periportal fibrosis. In addition, this group showed higher expression of IL-17 and IL-10 but lower IL-10/IL-13 ratio than patients in the WF/IFNE group. Cells from individuals who present any level of fibrosis expressed more TGF-β compared to the WF/IFNE group and a positive correlation with left lobe enlargement and portal vein wall thickness. There was a negative correlation between IL-17 and the thickness of the portal vein wall, but more studies are necessary in order to explore the possible protective role of this cytokine. Despite the fibrosis group having presented a higher expression of pro-fibrotic molecules compared to WF/IFNE patients, it seems there is a regulation through IL-10 and T reg cells that is able to maintain the low morbidity of this group.https://www.frontiersin.org/articles/10.3389/fimmu.2021.605235/fullschistosomiasisperiportal fibrosisSchistosoma mansoniCD4+ T lymphocytesfibrosis |
| spellingShingle | Jordana Batista Santana Tarcísio Vila Verde Santana de Almeida Diego Mota Lopes Brady Page Sergio Costa Oliveira Sergio Costa Oliveira Irismá Souza Luís Eduardo Viana Silva Ribeiro Néstor Adrián Guerrero Gutiérrez Edgar M. Carvalho Edgar M. Carvalho Edgar M. Carvalho Luciana Santos Cardoso Luciana Santos Cardoso Luciana Santos Cardoso Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis Frontiers in Immunology schistosomiasis periportal fibrosis Schistosoma mansoni CD4+ T lymphocytes fibrosis |
| title | Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis |
| title_full | Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis |
| title_fullStr | Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis |
| title_full_unstemmed | Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis |
| title_short | Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis |
| title_sort | phenotypic characterization of cd4 t lymphocytes in periportal fibrosis secondary to schistosomiasis |
| topic | schistosomiasis periportal fibrosis Schistosoma mansoni CD4+ T lymphocytes fibrosis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.605235/full |
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