An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging

Most fluorescent imaging systems use pre-defined filter sets with limited flexibility in the choice of excitation and emission bands and suffer from crosstalk between different fluorophores for multiplexed imaging. When implemented in the context of optical fibers for biological imaging in a remote...

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Main Authors: Shuyan Zhang, Jian Jun Chua, Wilbert Yao Hwee Tang, Joseph Yong Xin Cheng, Xiuting Li, Malini Olivo
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-01-01
Series:Frontiers in Physics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphy.2022.1096290/full
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author Shuyan Zhang
Jian Jun Chua
Jian Jun Chua
Wilbert Yao Hwee Tang
Wilbert Yao Hwee Tang
Joseph Yong Xin Cheng
Joseph Yong Xin Cheng
Xiuting Li
Malini Olivo
author_facet Shuyan Zhang
Jian Jun Chua
Jian Jun Chua
Wilbert Yao Hwee Tang
Wilbert Yao Hwee Tang
Joseph Yong Xin Cheng
Joseph Yong Xin Cheng
Xiuting Li
Malini Olivo
author_sort Shuyan Zhang
collection DOAJ
description Most fluorescent imaging systems use pre-defined filter sets with limited flexibility in the choice of excitation and emission bands and suffer from crosstalk between different fluorophores for multiplexed imaging. When implemented in the context of optical fibers for biological imaging in a remote setting with space constraints, the size of the fiber probe is crucial. To overcome these challenges, we combined the merits of hyperspectral imaging and an ultrathin optical imaging fiber where fluorescent images at 311 spectral bands in the visible range were captured using a liquid crystal tunable filter with a fiber probe of 500 μm in diameter. Fluorescent experiments were performed on quantum dots mixtures and genetically modified E. coli bacteria mixtures to demonstrate the system’s capabilities for multiplexed imaging. Images were first processed using a Fourier transform filtering technique to remove the fiber core pattern artifact. Subsequently, the classification of different types of bacteria samples was calculated using two methods, namely, spectral unmixing with maximum abundance and spectral matching with minimum spectral angle distance. Finally, the spatial distributions of individual bacteria types were overlaid with the mixture image, and the two classification results matched well. A simple-to-use graphic user interface (GUI) platform for the hyperspectral imaging fiber probe system was developed which performs image processing and displays the classification results. The methods and results presented will appeal to the optical fiber-based imaging and hyperspectral imaging communities in general and have great potential for biological imaging applications.
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spelling doaj.art-1c6522da80d14c2880f9f74babde53f82023-01-04T21:11:44ZengFrontiers Media S.A.Frontiers in Physics2296-424X2023-01-011010.3389/fphy.2022.10962901096290An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imagingShuyan Zhang0Jian Jun Chua1Jian Jun Chua2Wilbert Yao Hwee Tang3Wilbert Yao Hwee Tang4Joseph Yong Xin Cheng5Joseph Yong Xin Cheng6Xiuting Li7Malini Olivo8Translational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeDepartment of Biomedical Engineering, National University of Singapore, Singapore, SingaporeTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeDepartment of Biomedical Engineering, National University of Singapore, Singapore, SingaporeTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeDepartment of Biomedical Engineering, National University of Singapore, Singapore, SingaporeTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, A*STAR, Singapore, SingaporeMost fluorescent imaging systems use pre-defined filter sets with limited flexibility in the choice of excitation and emission bands and suffer from crosstalk between different fluorophores for multiplexed imaging. When implemented in the context of optical fibers for biological imaging in a remote setting with space constraints, the size of the fiber probe is crucial. To overcome these challenges, we combined the merits of hyperspectral imaging and an ultrathin optical imaging fiber where fluorescent images at 311 spectral bands in the visible range were captured using a liquid crystal tunable filter with a fiber probe of 500 μm in diameter. Fluorescent experiments were performed on quantum dots mixtures and genetically modified E. coli bacteria mixtures to demonstrate the system’s capabilities for multiplexed imaging. Images were first processed using a Fourier transform filtering technique to remove the fiber core pattern artifact. Subsequently, the classification of different types of bacteria samples was calculated using two methods, namely, spectral unmixing with maximum abundance and spectral matching with minimum spectral angle distance. Finally, the spatial distributions of individual bacteria types were overlaid with the mixture image, and the two classification results matched well. A simple-to-use graphic user interface (GUI) platform for the hyperspectral imaging fiber probe system was developed which performs image processing and displays the classification results. The methods and results presented will appeal to the optical fiber-based imaging and hyperspectral imaging communities in general and have great potential for biological imaging applications.https://www.frontiersin.org/articles/10.3389/fphy.2022.1096290/fullfiber sensorhyperspectral imagingfluorescent imagingspectral unmixingbioimagingquantum dots
spellingShingle Shuyan Zhang
Jian Jun Chua
Jian Jun Chua
Wilbert Yao Hwee Tang
Wilbert Yao Hwee Tang
Joseph Yong Xin Cheng
Joseph Yong Xin Cheng
Xiuting Li
Malini Olivo
An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
Frontiers in Physics
fiber sensor
hyperspectral imaging
fluorescent imaging
spectral unmixing
bioimaging
quantum dots
title An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
title_full An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
title_fullStr An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
title_full_unstemmed An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
title_short An ultrathin fiber-based fluorescent imaging probe based on hyperspectral imaging
title_sort ultrathin fiber based fluorescent imaging probe based on hyperspectral imaging
topic fiber sensor
hyperspectral imaging
fluorescent imaging
spectral unmixing
bioimaging
quantum dots
url https://www.frontiersin.org/articles/10.3389/fphy.2022.1096290/full
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